Anti-elastase Activity of Methanolic and Etyl Acetate Extract from Garcinia latissima Miq. Neneng Siti Silfi Ambarwati1, Berna Elya2*, Yesi Desmiaty3
1Department of Cosmetology, Faculty of Engineering, Universitas Negeri Jakarta, Jl. Rawamangun Muka, East Jakarta, 13220, Indonesia
2Department of Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, UI Depok Campus, Depok, 16424, Indonesia
3 Department of Pharmacognosy, Faculty of Pharmacy, Universitas Pancasila, Jl. Srengseng Sawah, Jagakarsa, Kota Jakarta Selatan, Daerah Khusus Ibukota Jakarta, 12640, Indonesia.
Abstract
Background. Elastase is a proteinase enzyme that can reduce elastin by dividing specific peptide bonds. Therefore, the inhibition of elastase activity in the dermis layer can be used to maintain skin elasticity. Materials that can inhibit elastase activity can be a cosmetic ingredient in dealing with skin aging. Increased desire to maintain healthy skin without chemicals, encourages the use of materials from natural sources such as plants from Indonesia, a country that has a high biodiversity, including Garcinia latissima from the Clusiaceae family. Purpose. The purpose of this study is to know G. latissima has the activity as an inhibitor of elastase enzyme or not. Methods. Plant extraction was carried out using maceration successively methods. The inhibition activity of elastase enzyme was carried out by measuring the kinetic enzyme N-succ (Ala) 3-Nitroanilide conversion to p-nitroaniline (substrate) spectrophotometry at 405 nm and using porcine pancreatic elastase (PPE) as an enzyme. Results. The results showed that the G. latissima methanol and ethyl acetate extract were active as elastase enzyme inhibitors. Conslusion. G. latissima extract can maintain skin elasticity.
