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121	Biology	ABS-770
Growth Pattern and Length-Weight Relationship of Rhyacichthys aspro in The Cisadea River, West Java, Indonesia Epa Paujiah (a*), Dedi Duryadi Solihin (b), Ridwan Affandi (c), Sumiyati Saadah (a) a) Department of Biology Education, Faculty of Tarbiyah and Teacher Training, UIN Sunan Gunung Djati Bandung Jl. Soekarno Hatta, No. 749, Cimincrang, Gede Bage, Bandung (Kampus II) b) Departement of Animal Biosciences, Bogor Agricultural University Kampus IPB Dramaga, Jl. Raya Dramaga, Babakan, Dramaga, Bogor, Jawa Barat, 16680 c) Departement of Water Resources Management, Faculty of Fish and Fisheries, Bogor Agricultural University Kampus IPB Dramaga, Jl. Raya Dramaga, Babakan, Dramaga, Bogor, Jawa Barat, 16680 *epapaujiah[at]uinsgd.ac.id Abstract Length-weight relationships or condition factor in R. aspro species were analyzed separately by sex and location. This study aims to determine the growth patterns and Length-weight relationships in the R. Aspro fish species that were captured in the Cisadea River, West Java, Indonesia. This research was conducted from June 2012 to February 2013. Fish sampling from the field was carried out for six months which took place from June to November 2012. Sampling was carried out at the station that had been determined by Purposive Sampling which could represent the upstream, middle and downstream of the river. The parameters measured consisted of growth patterns and factors conditions for fish (Length-weight relationships). The data obtained is then analyzed using the Excell 2007 computer program and uses the FAO-ICLARM Stock Assessment Tool II (FISAT II) program. The results showed that the correlation coefficient (r) R. aspro showed a value of 0.90 for male fish and 0.78 for female fish. The equation of the relationship between the length of weights in R. aspro and the different sexes all has a positive allomatic growth pattern (b> 3). Based on location, the fish growth pattern has positive and negative alomatric patterns. There are differences in conditions both based on gender and location. The relationship between R. aspro fish length is allometric with condition factor values ranging from 0.16-2.05. The results of this study can be used as baseline data for the management of aquatic resources in these locations or other locations. Keywords: Fish, Growth, R. aspro, Condition Factor, Cisadea River PermaLink | Plain Format | Corresponding Author (Epa Paujiah)

122	Biology	ABS-772
the effect of energy and protein balances on the digestive value of female garut lamb Pri Riznaya, Ana Rochana, Diding Latipudin Universitas Padjadjaran Abstract The purpose of this research was to determine the effect of energy and protein balances on the digestive value of female garut lamb. The variables are TDN (Total Digestible Nutrien) value, Crude Fiber (CF) digestive value and Crude Protein (CP) digestive value. The treatments given included R1 (12% protein and 60% TDN), R2 (12% protein and 65% TDN), R3 (14% protein and 60% TDN), R4 (14% protein and 65% TDN), R5 (16% protein and 60% TDN), and R6 (16% protein and 65% TDN). The feed are arrange using field grass, fine bran, onggok, soy sauce pulp, coconut cake, and premix. The design of the room uses a Completely Randomized Design (CRD), with 4 (four) replications. Data analysis used variance analysis and Duncans multiple analysis. The results showed that various protein and energy ratio had significant effect on TDN (Total Digestible Nutrien) value, Crude Fiber (CF) digestive value and Crude Protein (CP) digestive value. Keywords: : Garut lamb, protein and energy balances, digestive value PermaLink | Plain Format | Corresponding Author (Pri Riznaya)

123	Biology	ABS-5
THE EVALUATION OF STERILIZATION PROTOCOL FOR SPROUT EXPLANTS IN OIL PALM (Elaeis guineensis Jaq.) TISSUE CULTURE Neliyati Neliyati, Lizawati Lizawati, Zukarnain Zulkarnain* Faculty of Agriculture University of Jambi Jl. Raya Jambi Muara Bulian KM 15, Mendalo Indah, Jambi, Indonesia *Email: dr.zulkarnain[at]yahoo.com Abstract The objective of this study was to develop an efficient protocol for sterilization of sprout explants in tissue culture of oil palm. Plant materials used were plumulae and radicle from sprouts of the D x P Sriwijaya variety obtained from Seed Processing Unit PT Binasawit Makmur, Palembang. The medium used was MS composition was supplemented with vitamins, myo-inositol and sucrose, and the pH was set at 5.8 \pm\frac{-b\pm\sqrt{b^2-4ac}}{2a} 0.02 before being solidified with agar. Cultures were maintained under a light intensity of 1,500 lux and 16-hour photoperiod and temperature of 24 hingga 26 oC. Five protocols for the eradication of explant contamination were tested. i.e. There were 5 (five) different methods of explant sterilization employed, ie: A) explants were washed with sterile aquadest followed by soaking in 70% alcohol for 1 minute; B) explants were washed with sterile aquadest followed by soaking in 1% Benlox 50WP solution for 30 minutes, 1% Agrept 20WP for 30 minutes, and soaking in 70% alcohol for 5 minutes; C) explants were washed with sterile water plus detergent, followed by soaking in 1% Benlox 50WP plus few drops of Tween-80 for 30 minutes, soaking in 1% Agrept 20WP plus few drops of Tween-80 for 30 minutes, soaking in 70% alcohol for 5 minutes; D) explants were washed with sterile water plus detergent, followed by soaking in 0.1% HgCl2 solution for 30 minutes; and E) explants were washed with sterile water plus detergent, followed by soaking in 0.2% Dithane M-45 plus few drops of Tween-80 for 30 minutes, soaking in 1% NaOCl for 5 minutes, soaking in 0.1% HgCl2 plus few drops of Tween-80 for 30 minutes. The results showed that treating the explants with 0.1% HgCl2 for 30 minutes following 0.2% Dithane M-45 and 1% NaOCl applications was proven to be effective for the eradication of contamination. Keywords: oleaginous plant, in vitro culture, micropropagation, plant propagation PermaLink | Plain Format | Corresponding Author (Zulkarnain Zulkarnain)

124	Biology	ABS-1030
Antimicrobial activity of Aspergillus sp fungi isolated from land waste disposal in Bogor, Indonesia on the growth of Staphylococcus aureus, Salmonella thyphosa and Candida albicans. Dalia Sukmawati1,2* and Novitha Noorhadiatiningsih3 1) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia. 2) Universitas Negeri Jakarta Culture Collection (UNJCC), 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia. 3) Universitas Muhammadiyah Prof. DR. HAMKA, Klender. Abstract Aspergillus sp. mold is found in various habitats, especially on the ground. Land waste disposal is a good medium for the growth of microorganisms due to the condition of the land that is rich in organic matter. This study is expected to provide information about the potential of Aspergillus sp. mold mainly from soil which can produce antimicrobial compounds. This study aims to determine whether the metabolic metabolites of Aspergillus sp. obtained have activities as antimicrobials against Staphylococcus aureus, Salmonella thyphosa and Candida albicans. The fermentation was carried out with a static fermentation method which was incubated for 5 days and the antimicrobial activity test was carried out by the diffusion method to use cylinders with 3 replications. The results of macroscopic and microscopic isolation and identification obtained five isolates from genera of Aspergillus sp. which were coded N1, N2, N3, N4 and N5. The metabolites were obtained from the fermentation of each fungus isolate Aspergillus sp. The test results of the metabolite activity of fungus Aspergillus sp. had antibacterial activity, against Staphylococcus aureus, Salmonella thyphosa and Candida albicans. Keywords: Antimicrobial, Aspergillus sp., Static Fermentation. PermaLink | Plain Format | Corresponding Author (Dalia Sukmawati)

125	Biology	ABS-778
SCREENING OF BIOSURFACTANT PRODUCING BACTERIA FROM HYDROCARBON CONTAMINATED SOIL Tri Handayani Kurniati, Sri Rahayu, Dalia Sukmawati, Wury Maharani Biology Study Program, Faculty of Mathematics and Natural Sciences, Jakarta State University. Jl, Rawamangun Muka, Rawamangun,. East Jakarta 13220. Indonesia Abstract Biosurfactants are surface-active molecule produced by microorganisms and have several advantages over the chemical surfactants. The objective of this research was to obtain biosurfactant-producing bacteria from hydrocarbon contaminated soils in Jakarta Bay and to observe their biosurfactant activity through emulsification capacity and surface tension reduction capability. Overall, 19 isolates were screened for biosurfactant production by hemolysis assay, drop collapse assay and oil spreading assay. The result showed that 12 strains gave positive results on the screening tests and were determined as biosurfactant-producing bacteria. Three strains (TKY 3, TKY 14 and TKY 19) had the best biosurfactant activity. Isolate TKY 3 has the best emulsification index (E24) with a value 57±0,57%, while the lowest surface tension value was achieved by two strains ( TKY 19 and TKY 14 ) with the same value of 37,84±1,45 mN/m. Keywords: bacteria; biosurfactant; emulsification; screening; surface tension PermaLink | Plain Format | Corresponding Author (Tri Handayani Kurniati)

126	Biology	ABS-11
The Detection of Pork Contamination in the Beef Sausage Products in Yogyakarta City with Polymerase Chain Reaction Method Vallery Athalia Priyanka (a*), Susana Ristiarini (b), Pramana Yuda (c) a) Department of Biology, Faculty of Mathematics and Science Nature, Universitas Indonesia, Jl. Prof. Dr. Sudjono D Pusponegoro, Depok 16424, Indonesia *vallery.athalia[at]ui.ac.id b) Departement of Food Technology, Faculty of Agricultural Technology, Widya Mandala Catholic University Surabaya, Jl Dinoyo 42-44, Surabaya 60625, Indonesia c) Departement of Biology, Faculty of Biotechnology, Atma Jaya University Yogyakarta, Jl. Babarsari no 44, Sleman 55281, Indonesia Abstract Meat-based food products are at risk for other meat mixtures that are difficult to detect with the naked eye. The advancement of molecular technology offers the convenience of detecting contaminants in food products. The purpose of this study was to detect pork contamination in beef sausage from several markets in Yogyakarta City using PCR and sequencing, and knowing effectiveness and sensitivity primers. The study using 6 primers, consisting of 2 universal mammals and 4 specific pigs. Samples used were 9 sausages. Seven samples from Yogyakarta, 2 samples from Thailand. Positive samples of mammalian DNA with universal primary mammals namely P195 and CB by 100 % and 0 %. CB primers amplify mammalian DNA in all samples with no specificity. Percentage of pig DNA with P14, PPA6, PPA8 and pork specific primers were 88.89 %, 22.22 %, 22.22 % and 22.22 % respectively. PPA6, PPA8, and pork amplify pig DNA are not specific, whereas P14 is specific, which tested for sensitivity. Positive pig sample was made by varying concentrations of 1, 2, 5, 10, 15, 20, 25, and 100 %. P14 can detecting up to 1 %. Positive pig sample were taken for sequencing. Sequencing shows Sus scrofa was detected. Keywords: BLAST; primer P14; sequencing; Sus scrofa PermaLink | Plain Format | Corresponding Author (Vallery Athalia Priyanka)

127	Biology	ABS-781
ABILITY OF GARLIC EXTRACT AND KAEMPFERIA GALANGAL INHIBIT ASPERGILLUS sp. ISOLATED FROM SARDINE FISH PEDETAN Ni Made Ayu Suardani Singapurwa, I Putu Candra, I Nyoman Rudianta Department of Food Science and Technology, Warmadewa University Abstract ‘Pedetan’ is one of the food products of the traditional Balinese fish sardine which is processed by the community in the Jembrana Regency of Bali Province. This study aims to determine the antimicrobial activity of garlic and kaempferia galangal in inhibiting the growth of Aspergillus sp. which can contaminate the sardine fish Pedetan, The study was conducted by isolating Aspergillus sp. from Pedetan and testing the inhibitory power of garlic and Kaempferia galangal The results showed that the extract of garlic and Kaempferia galangal can inhibit the growth of Aspergillus sp. From the results of SEM observations it was found that garlic and kaemfiera were able to damage the Aspergillus sp. Mycelia. compared to control treatment. Garlic extract and kaempferia galangal can inhibit the growth of Aspergillus sp. because they contains bioactive compounds that are antimicrobial. Keywords: Aspergillus sp., Garlic, Kaempferia galangal PermaLink | Plain Format | Corresponding Author (Ni Made Ayu Suardani Singapurwa)

128	Biology	ABS-271
Factors That Influence The Event Of Low Birth Weight Baby (LBW) In Mother At Payangan, Bali Asri Lestarini (a*), I Dewa Gede Agung Suta Ariwangsa (a) a) Faculty of Medicine and Health Sciences, Warmadewa University Jalan Terompong no 24, Denpasar, Bali 80235 *asrilestarini[at]gmail.com Abstract The main predisposing factor of infant mortality is Low Birth Weight (LBW). LBW can have long-term effects on child developments, therefore the high incidence of low birth weight can affect the quality of human resources. The purpose of this study was to determine the predisposing factors of the occurrence of LBW in Payangan sub-district, Bali. This research was analytical research with a case-control design to assess predisposing factors of the occurrence of LBW by assessing medical records of pregnant women. The sample of this study was 92 mothers with a history of childbirth. Chi-square method was done to test the significance of each variable on the independent variable. Results obtained from five predisposing factors, those which had correlations to the incidence of LBW in Payangan Health Center were twin births, nutritional status, and anemia with a P value of <0.05. The conclusion made from the results of this study was the variables that have significant effect were variables of twin births, nutritional status, and anemia, while parity did not have a significant effect. A Suggestion made that it can provide benefits to health workers at health centers and pregnant women about LBW risk factors. Keywords: low birth weight, nutrition PermaLink | Plain Format | Corresponding Author (Asri Lestarini)

129	Biology	ABS-273
THE DENSITY COMPARISON OF JAVANESE EDELWEIS (Anaphalis javanica) IN TEGAL ALUN, TEGAL BUNGBRUN AND PONDOK SALADAH OF THE PAPANDAYAN MOUNTAIN GARUT L. Amalia, W. L. Hakim, Miranti, D. I. Putri and T. Kristianti Institut Pendidikan Indonesia Garut Abstract Javanese Edelweis (Anaphalis javanica) is a pioneer plant that has enormous ecological benefits. This plant is living on volcanic ash deposits and crater soils as on Mount Papandayan. The presence of Javanese Edelweis are endangered. This study aims to: (1) compare the density of Javanese Edelweis on Mount Papandayan especially in Tegal Alun, Tegal Bungbrun and Pondok Saladah; (2) knowing the effect of abiotic factors (temperature, light intensity and soil pH) on the density of Javanese Edelweis. The method used in this research is quantitative descriptive research method. Data collection was carried out using the 100 meter belt transect method with a width of 5 meters in three sample locations: Tegal Alun (28 transects), Tegal Bungbrun (3 transects) and Pondok Saladah (10 transects). The results indicate that there is a difference in the density of Javanese Edelweis between TegalAlun, Tegal Bungbrun and Pondok Saladah. The density value in Tegal Alun is 2275.7 trees/hectare, Tegal Bungbrun 946.7 trees/hectare and Pondok Saladah 366 trees/hectare. Whereas for abiotic factors it is known that air temperature, light intensity and soil pH simultaneously influence the density of Javanese Edelweis with R value 85% and influence 70%. Keywords: Anaphalis javanica, Javanese Edelweis, abiotic factors, density. PermaLink | Plain Format | Corresponding Author (Lida Amalia)

130	Biology	ABS-274
Insecticidal Activity of Organophosphate Chlorpyrifos and Essential Oils of Andropogon nardus, Eucalyptus globulus, and Mentha piperita Against Stored Pest Callosobruchus maculatus (F.) Niken Subekti (a*). Hadi Nur (b). Alfath Fanidya (a). Susanti (a). Rizka Saputri (a). Putri Indrawati (a). a) Biology Department, FMIPA, Universitas Negeri Semarang Jalan Taman Siswa, Semarang 50229, Indonesia *nikensubektiunnes[at]gmail.com b) Ibnu Sina Institute for Fundamental Science Studies, Universiti Teknologi Malaysia, 81310 UTM Skudai, Johor, Malaysia Abstract Callosobruchus maculatus (F.) is a stored pest that cause demage to nuts. One of the techniques of preservation of foodstuffs from the insects pest that already done is by using chemical pesticides and fumigant. The use of chemicals continuously can affect to peoples health and pollute the environment and non-target organisms. Pest control using essential oils is considered safer and environmentally friendly. Essential oils used in this study are A. nardus, E. globulus, and M. piperita. It made nanoemultion to reduce evaporation. This research aims to analyze the insecticidal activity of chlorpyrifos and the three types of essential oils as well as their repellent to adult Callosobruchus maculatus (F.). The research method consists of sample preparation, manufacture nanoemultion of essential oils, mortality test, and repellent test. The results showed that the pesticide chlorpyrifos has high insecticidal percentage compared with essential oils, but its use is not environmentally friendly. However, all the encapsulated essential oils in this study has potential to be used as a environmentally friendly pesticides to control the insect pest Callosobruchus maculatus (F.). Keywords: Callosobruchus maculatus (F.), chlorpyrifos, essential oil PermaLink | Plain Format | Corresponding Author (Niken Subekti)

131	Biology	ABS-23
The Evaluation of Various Nutrient Formulation on The Growth of Lettuce in Hydroponic Raft System at Tropic Region Budy Frasetya(a*), Ahmad Taofik(a), Maratun Sholehah(a) a) Agrotechnology Department, Faculty of Science and Technology, UIN Sunan Gunung Djati, Jalan A. H. Nasution 105, Bandung 40614, Indonesia *)budyftq1682[at]gmail.com Abstract Lettuce is leaf vegetable which consumes as a fresh vegetable. The quality is a crucial role in increasing the market price of lettuce. A large number of nutrient solution is sometimes confusing the farmer or grower which will they choose. The research objectives evaluate five nutrient formulation from local formulator and global formulator.. This reseach conduted in June to July 2018 at Greenhouse of Universitas Padjadjaran, Jatinangor. The experimental design used in this research was completely randomized design with five treatments of nutrient formulation e.g., Sutiyoso (local formulator), Hoagland & Snyder, Hoagland, Shive & Robbins, and Resh Lettuce Anguila. The parameters of lettuce growth (plant height, leaves a number, leaf area, fresh weight of the plant, dry weight of the plant) observe and measure. The data of lettuce plant growth than analyzed with variance analysis α=5% and post hoc test analysis use Duncan multiple range test α=5%. Each of the treatment replicate five times. The results showed that different of nutrient formulations for lettuce have a different effect on growth parameters such as of plant height, leaves a number, leaf area, fresh weight of the plant, dry weight of the plant. The Sutiyoso nutrient formula effective for tropical lettuce hydroponics in Indonesia. Keywords: tropic hydroponics, electrical conductivity, lettuce raft system, local formulation PermaLink | Plain Format | Corresponding Author (Budy Frasetya)

132	Biology	ABS-535
Capabilities Yeast Isolated From Indigenous Fermented Beverages Of Indonesia To Producing Amylase Enzyme Dalia Sukmawati(a,b*), Zico Arman(a), Zakiah Nur Afifah (b), Mutia Balqis (b), Hesham El Enshasy (c, d), Sri Rahayu (b), Tri Handayani (b), Rini Puspitaningrum (b) a) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia b) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia c) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia d) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia Abstract Indonesia has many fermented beverages and yeast become one of the agent in fermented process. Yeasts has a role to transform carbohydrate complex into simple compound with release secondary metabolism to environment like amylase enzyme. This study aim to get the isolate of yeast that can potentially produce amylase enzyme. This research conducted in Oktober 2018 until Maret 2019 in Microbiology Laboratory of Universitas Negeri Jakarta. The screening test of potential isolate producing amylase enzyme was perfomed on yeast isolate from eight source indigenous fermented beverages that can grow in YMA medium with pH 2. Screening was carried out on YPSA medium with difussion agar method. From 53 Isolates, there is 16 isolates were able to form clear zone during one day incubation in room temperature there are IB4, IB15, IB20, IB21, IB26, IB36, IL78, IL80, IL81, IL86, IL88, IL97, IL113, IL136, IL146, and IL150, the highest amylolytic index was produce by IL86 (1,019 U/ml). Forming the clear zone is proof that yeast can transform starch become simply sugar like maltose because iodine-starch reaction performed amylose helix and iodine become I3 that filled main core helix, even iodine forms complexes with starch molecules showed a dark purple colour Keywords: Amylase, Fermented, Yeast PermaLink | Plain Format | Corresponding Author (Zico Arman)

133	Biology	ABS-536
Screening of Cellulase Producing Yeast Isolated from Indigenous Fermented Beverages of Indonesia Dalia Sukmawati(a)(b), Gibran Sondana(b), Nabila Nov(b), Rifdah Hasanah(a), Hesham El Enshasy(c),(d), Sri Rahayu(b), Tri Handayani(b), and Rini Puspitaningrum(b) (a) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia (b) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia (c) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia (c) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia Abstract The aim of this study is to demonstrate screening isolates of yeast with high cellulase activity from indigenous fermented beverages of Indonesia. This research conducted in Oktober 2018 until Maret 2019 in Microbiology Laboratory of Universitas Negeri Jakarta. The screening test of potential isolate to producing cellulase enzyme was perfomed on 53 yeast isolate from eight source indigenous fermented beverages that can grow in YMA medium with pH 2. Screening was carried out on CMC medium with difussion agar method. The cellulose activity of the organisms was measured diameter of a clear zone around the colony on Carboxymethyl Cellulose (CMC) agar media. The result of screening potential isolate to producing cellulase showed from 50 isolates, 17 isolate of yeast performed has potentially cellulose activity. They were selected according to their capabilities of degrading carboxymethyl cellulose (CMC). Among these 17 strains, 3 strains namely IL112, IL125 and IL78 showed higher levels of enzyme activity based on celulolytic index value. Keywords: Yeast, Fermented, Cellulase PermaLink | Plain Format | Corresponding Author (Rifdah Hasanah)

134	Biology	ABS-283
Screening of Ligninolytic Activity of Some Basidiomycota from Domestic Oil Palm Plantation in Bogor N.S. Sepwin, A. Fijai & Y. Mulyaningsih Faculty of Engineering, Sampoerna University Jl. Pasar Raya Minggu No 16, Jakarta Selatan, Indonesia Abstract Lignin is an essential bio-polymer which is required in the formation of plant cell wall. The degradation of lignin in the nature is a major challenge due to the complexity of its structure. It has been long known that white rot fungi produce ligninolytic enzyme that can degrade lignin efficiently. To identify white rot fungi, screening test was carried out by testing different strains of Basidiomycota using czapek dox agar supplemented with Guaiacol (0,02%). The results showed that two out of thirteen strains of Basidiomycota have ligninolytic activity. In this study, we successfully obtained two strains that have ligninolytic activity. This study sheds light on further observation of these strains which potentially can be used for lignocellulose waste treatment especially oil palm empty fruit bunch (OPEFB). Keywords: basidiomycota, degradation, ligninolytic, white rot fungi PermaLink | Plain Format | Corresponding Author (sepwin sitompul)

135	Biology	ABS-795
LIANAS DIVERSITY IN GAMBUNG AREA GUNUNG TILU NATURE RESERVE Tri Cahyanto(a), Astri Yuliawati(b*), Noneng Fitriyatul Adawiyah(a), Dede Fajar(a) a) Biology Department, Universitas Islam Negeri Sunan Gunung Djati, Indonesia b) Biology Education Department, Universitas Islam Negeri Sunan Gunung Djati, Indonesia astriyuliawati[at]uinsgd.ac.id Abstract Lianas is one of conspicuous plant in tropical rainforests. Its special characteristics is climbing or twisting around other plant. Gunung Tilu nature reserve is one of remaining tropical forest in West Java which potentially as lianas habitat. This research aims are inventory and determine the diversity of lianas in Gambung area which is the most potential area for lianas habitat in Gunung Tilu nature reserve. This research was carried out by descriptive exploratory method. Sampling was done by combining transect and quadrat technique method, with length of the transect is 700m which stretched from an altitude of 1489mpl (the lowest altitude at the study site) to 1580mdpl (highest altitude). The results showed that there were 13 families, 15 genera with 24 species of lianas. Tetrastigma sp. is the type of liana with the highest Important Value Index of 29.24%, while Aescyhnanthus sp.2 and Cissampelos pareira have the lowest Important Value Index of 0.47%. Lianas Shannon – Wiener diversity index in Gambung area Gunung Tilu nature reserve is 1.092. Keywords: Liana, vegetation analysis, diversity PermaLink | Plain Format | Corresponding Author (Astri Yuliawati)

136	Biology	ABS-29
Effect of Java Long Pepper Extract (Piper Retrofractum) on Spermatozoa Motility of Mustika Common Carp (Cyprinus Carpio L.) Bela Berli Yeni(a*) and Abinawanto(b) a)Program of Pascasarjana Biology, Universitas Indonesia, Jl Profesor Doktor Sudjono, Depok 16424, Indonesia *bela.lim440[at]gmail.com b)Department of Biologi, Universitas Indonesia, Jl Profesor Doktor Sudjono, Depok 16424, Indonesia Abstract Java long pepper is a plant has an aphrodisiac effect. Its extract contains cytosterol and piperine which capable of affecting the reproductive hormones in male Siamese catfish. The purpose of study to evaluate the effect of java long pepper extract on spermatozoa motility of mustika common carp. Java long pepper extract was injected intramuscularly in the first of week with doses of 0.9 ml kg, 1.8 ml kg and 3.6 ml of body weight, respectively. Ovaprim in a dose of 0.3 ml body weight was administered as a positive control, whereas 0.1 ml of sodium solution was applied as a negative one. The result was observed in the second of week. Based on the ANOVA test showed that java long pepper extract has a significant effect on spermatozoa motility of mustika common carp (P<0.05). Further, according to the Tukey test that the highest spermatozoa motility rate was yield from a dose of 1.8 ml of java long pepper extract kg by body weight 69.5%. Keywords: Java long pepper extract; Spermatozoa motility; Mustika common carp PermaLink | Plain Format | Corresponding Author (Bela Berli Yeni)

137	Biology	ABS-288
Antioxidant Activity of Ethanolic Extract, n-Hexane Fraction, Ethyl Acetate Fraction and Water Fraction of Garut Orange Leaves (Citrus reticulata Blanco) Faizah Min Fadhlillah, Wiwi Oktaviani and Ria Mariani Garut University Abstract Antioxidants are compounds that are able to eliminate, cleanse, and resist radical effects. Antioxidants can stabilize free radicals by complementing the lack of electrons possessed by free radicals. Several studies have shown that citrus leaves of other spesies have been shown to have antioxidant activity. This study aims to determine the antioxidant activity of ethanol extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves (Citrus reticulata Blanco) using DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. Garut orange leaves were extracted with ethanol 96% by macerator apparatus. Concentrated extracts were fractionated by liquid liquid extraction method with n-hexane, ethyl acetate and water solvent. Furthermore, the ethanolic extract, n-hexane fraction, ethyl acetate fraction and water fraction of garut orange leaves were tested for antioxidant activity using the DPPH (2,2-Diphenyl-1-Picrylhydrazyl) method. The antioxidant activity test results showed that ethanolic extract of garut orange leaves had better antioxidant activity, followed by n-hexane fraction, ethyl acetate fraction and water fraction. Keywords: garut orange leaves, antioxidant activity, DPPH PermaLink | Plain Format | Corresponding Author (Faizah Min Fadhlillah)

138	Biology	ABS-544
Jigsaw Learning Model base on Cooperative Instructional Strategies to Improve Academic Discussion in Adult Education on Environment concepts Durotul Yatimah, Solihin a*), Adman and Rahmat Department of Out-of-School Education, Faculty of Education, Jakarta State University Biology Education – Faculty of Mathematics and Natural Sciences – Jakarta Islamic State University* solihinone[at]yahoo.com Indonesian education university. Dr. Styabudi 229 Bandung Asmis business and multimedia institute Abstract This study investigated the contribution of a Cooperative Instructional Strategies in developing academic discussion. Academic discussion is an important element of historical thinking, which is considered to improve conceptual understanding and support the development of democratic citizenships by helping students interpret, understand and connect patterns of human activity across time in environment. Adult learner as research conditions, participated in this study. The outcome variables discussed in the paper are adult learner self-esteem and classroom climate. Over the period of the study, cooperative learning did not significantly improve the self-esteem of adult learner. However, adult learner in the experimental group who were taught through the cooperative learning approach perceived classwork to be less difficult than the control adult learner who worked individually. The control adult learner also reported a decline in satisfaction with classwork and perceived more friction in their class. Interviews with the instructor and adult learner showed favourable attitudes toward cooperative learning. Keywords: Cooperative learning, classroom climate, environment, self-esteem, affective outcomes, adult learner perceptions PermaLink | Plain Format | Corresponding Author (solihin solihin)

139	Biology	ABS-545
Innovation Works of Critical Impact Training Model Based on Mass Media to Improve The Capability of Environmental Critical Learning for Students of The Critical Pedagogics Group of Jakarta State University Durotul Yatimah, Solihin (a*), Adman and Rahmat Department of Out-of-School Education, Faculty of Education, Jakarta State University Biology Education – Faculty of Mathematics and Natural Sciences – Jakarta Islamic State University* solihinone[at]yahoo.com Indonesian education university. Dr. Styabudi 229 Bandung Asmis business and multimedia institute Abstract Students critical thinking skills in environmental concepts can be improved by one of them by realizing learning through critical literacy training based on mass media. The research was conducted in two places, first at the Didactic LPM Secretariat, UNJ as a routine meeting place and the second at the NGO Unlimited Schools office on Jalan Kemandoran. The purpose of this study was to obtain empirical information regarding the improvement of critical learning skills of members of critical pedagogy groups. The methodology used in this study is Practical Action Research or action research by organizing a learning action in a training model. The results of the research obtained showed an increase in critical learning skills of members of critical pedagogy groups after being given treatment that had been tested by hypothesis with the t-test. The average increase in their critical learning abilities reaches 43%. The conclusion that can be drawn is that this study tends to be able to be a solution to the problems of critical learning that are used functionally in other objects. Keywords: Critical Learning Environment, Students, Training Model PermaLink | Plain Format | Corresponding Author (solihin solihin)

140	Biology	ABS-547
Management Strategy of Atrial Fibrillation with Wide QRS Complexes in Wolff-Parkinson-White Syndrome: a Case Report Reeiner M,Pranata IGB,Bagiari KE Departemen of Cardiology, Sanjiwani Hospital and Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Abstract. Wolff-Parkinson-White (WPW) syndrome is one of the most common pre-excitation syndrome. Accessory pathway formed between the atria and ventricles become conduction path other than the atrioventricular node. The occurrence of atrial fibrillation (AF) in the WPW syndrome cases are quite rare, and seldom reported in the medical literature. WPW syndrome that have a tachyarrhythmia has a specific treatment and contraindications, thus giving preferred treatment should avoid the potential occurrence of ventricular fibrillation (VF). We describe adult male presenting with a very rapid, irregular wide QRS complex tachycardia, with hemodynamic instability. Successful cardioversion performed in the emergency room (ER). ECG results after cardioversion showed normal sinus rhythm with accompanying evidence of accessory pathway in accordance with WPW type A with the localization of the LV free wall. Medical personnel need special attention in emergency cases. AF with aberrant conduction and ventricular tachycardia are the very first thing to be considered in cases of wide QRS complex tachycardia. However, medical personnel need to consider that WPW syndrome accompanied with AF will give an increased heart rate (> 200 x / min), wide QRS complex and irregular rhythm, especially when choosing a pharmacological therapy. Several hypotheses which can cause AF in patients with WPW including spontaneous degeneration atrioventicular reciprocating tachycardia (AVRT), electrical properties accessory pathway, accessory pathway effects on atrial structure and vulnerability of intrinsic atrial muscle. The location of accessory pathway and also the possibility of a hidden track of accessory pathway that sometimes gives an overview of less obvious delta waves, but does not rule out the WPW syndrome in this case. AF has a significant prognostic in WPW syndrome. This condition can be life-threatening if there is very rapid ventricular response. The use of drugs that slow AV conduction block is not recommended. Keywords: management; atrial fibrillation; WPW syndro PermaLink | Plain Format | Corresponding Author (erna bagiari)

141	Biology	ABS-548
Dilated Cardiomyopathy with Long QT secondary to Hypothyroidism and Hypocalcaemia in Patient with Post Total Thyroidectomy: A Case Report Komang Aditya Yudistira, Pranata IGB, Ketut Erna Bagiari Department of Cardiology, Sanjiwani Hospital/ Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Abstract The association between lack of thyroid hormones and cardiac dysfunction has been well described. We experienced a case of a 44-year-old woman with dilated cardiomyopathy (DCM) accompanied with long QT secondary to hypothyroidism and hypocalcaemia. Thyroid hormone acts on the myocardium of the heart and peripheral blood vessels. The genomic and non-genomic effects of thyroid hormone are associated with cardiovascular and chemodynamic function. A well documented but mostly overlooked sign in hypothyroidism is the prolongation of the QT interval. Long-QT syndrome, which is characterized by abnormal QT-interval prolongation on the surface ECG and an increased risk of sudden death, usually due to ventricular fibrillation. The mechanism of hypothyroidism on QT prolongation and the occurrence of ventricular tachycardia is unknown and the effect may be idiosyncratic, although coexistent. Keywords: Delated cardyomyopathy; Long QT syndrome; Total Thyroidectomy PermaLink | Plain Format | Corresponding Author (erna bagiari)

142	Biology	ABS-296
CORRELATION BETWEEN TUBERCULOSIS AND NUTRITIONAL STATUS AMONG CHILDREN IN NORTH LOMBOK, WEST NUSA TENGGARA, INDONESIA Putu Indah Budi Apsari, Bela Siska Afrida, Melissa Ronaldi, I Nyoman Supadma Faculty of medicine and Health Sciences, Warmadewa University Abstract Tuberculosis (TB) and malnutrition are the main causes of morbidity and mortality among children in developing countries. Tuberculosis become worse if there are present of malnutrition. In contrary, malnutrition can be caused by chronic inflammatory disease such as TB. This study aimed to analyze the correlation between tuberculosis and nutritional status among children in North Lombok, West Nusa Tenggara, Indonesia. This cross sectional study recruited child TB aged under 18 years as participants. TB was diagnosed by TB scoring in Children, Tuberculin Test, Fine Needle Aspiration Biopsy (FNAB) and Thorax Photos, and nutritional status was assessed using Center for Disease Control and Prevention (CDC) chart. Total 130 children were identified, 65 children with TB and 65 healthy children as control. This study showed that pulmonary TB was present in 72.3% of case, Lymphadenitis TB was 16%, and mixed pulmonary and lymphadenitis TB were 2%. Under-nutrition child was found in 63.1% of cases, malnutrition child was found in 29.2% of cases. There was significant correlation between tuberculosis and malnutrition among children (p<0.05). The highest proportion of TB was in children under five years (52.3%). TB is strongly correlated with malnutrition among children. Keywords: malnutrition, tuberculosis, children PermaLink | Plain Format | Corresponding Author (Putu Indah Budi Apsari)

143	Biology	ABS-557
Relationship Between Junk Food Consumption With Obesity In Children In West Denpasar District AAA L Paramasatiari and KA P Angela Faculty of Medicine, Warmadewa University Abstract Introduction: Changes in food consumption patterns in children is that they are currently consuming instant foods and having poor nutritional value. This causes the prevalence of obesity in children to increase every year. It continues into adulthood so it is important to carry out interventions to prevent overweight and obesity in children. Objective: This study aims to determine the relationship between consumption of junk food and childhood obesity. Method: This study was a cross sectional study in children aged 9-12 years in West Denpasar District with 175 respondents selected by simple two-stage cluster random sampling. Respondents were children aged 9-12 years who attended elementary schools in West Denpasar District without a history of obesity in their parents. The obesity criterion in this study is if the Body Mass Index (BMI) according to age is more than Z score 2. Criteria for consumption of junk food is if at least once a week consume low-nutrition foods that contain fat and soft drinks. The collected data was analyzed with SPSS 21 for window including Chi-Square test to look for the relationship of consumption of junk food with obesity and multiple logistic regression analysis to control the influence of variables on parental education, parental income and physical activity of children. Result: Obesity prevalence was 18.3%. The prevalence of obesity in the group that consumes junk food is 12%, while in the group that does not consume junk food is 6.3%. There was a significant relationship between consumption of junk food and the prevalence of obesity (x2 = 4,65; p=0,031) with Odds Ratio 2.56 and 95% Trust Interval 1.15-5.72. After being controlled by the parents education variables and childrens physical activity, this relationship was still significant (p = 0.015) but the adjusted Odds Ratio was only 0.32. Conclusion: there is a moderate positive relationship between consumption of junk food and obesity in children. Keywords: junk food, risk factors,and obesity PermaLink | Plain Format | Corresponding Author (Anak Agung Ayu Lila Paramasatiari)

144	Biology	ABS-47
Abundance of Green algae in The Intertidal Zone, Karang Papak Coastal, West Java, Indonesia Tri Cahyanto (a), Epa Paujiah (b*), Ingga Rinasti (a) a) Department of Biology, Faculty of Sciences and Technology, UIN Sunan Gunung Djati Bandung Jl. AH. Nasution, No. 105, Cibiru, Bandung b) Department of Biology Education, Faculty of Tarbiyah and Teacher Training, UIN Sunan Gunung Djati Bandung Jl. Soekarno Hatta, No. 750, Gede Bage, Bandung *epapaujiah[at]uinsgd.ac.id Abstract Green alge (Chlorophyta) is one of macroalgae who have much function such as ecologically and economically living in the intertidal zone. Purpose of this research want to know about abundance of green algae group in the intertidal zone, Karang Papak Coastal, West Java, Indonesia. The research was carried out in November 2018 every week (following moon phase) with transect methods. The results showed that the Karang Papak Coastal have 15 Species with genus of Ulva who have highest abundance than other group. Its can be caused by several factor such micro and macro climate (Temperate). This data can be use for complete data about Indonesian macrolagae. Keywords: Abundance, Indonesia, Intertidal Zone, Karang Papak Coastal, Macroalgae, PermaLink | Plain Format | Corresponding Author (Epa Paujiah)

145	Biology	ABS-563
Risk factors of coronary heart disease at Sanjiwani Hospital Bali Agus Santosa, P Windhu Mahayana Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Coronary heart disease is a condition with inadequate blood supply to the cardiac muscle caused by coronary arteries obstruction. CHD as one of the cardiovascular disease was the highest cause of death in Indonesia from 1995 to 2007. Based on Indonesia National Research in 2014, Bali was placed on the seventeenth position with 12.272 CHD patients. Risk of CHD can be reduced by prevention and control of the risk factors. The purpose of this study was to find out the risk factors of CHD patients hospitalized in Sanjiwani Gianyar Hospital. This descriptive study used cross sectional design. Data collection were carried out based on the medical records of CHD patients hospitalized in Sanjiwani Gianyar Hospital from January to December 2016, which were selected by simple random sampling technique. The study research showed the majority (91,3%) of CHD patients were aged ≥ 45, male (58%), and had no family history with cardiovascular disease (78,3%). Most CHD patients had no smoking habit (68,1%), had normal BMI (68,1%), had no diabetes mellitus history (65,2%), and had hypertension history (55,1%). Most (84,1%) of CHD patients had normal value of total cholesterol level, normal value of trigliserida level (62,3%), sub optimal LDL level (52,2%), and low HDL level (34,8%). This concluded that most risk factors for CHD patients were aged ≥ 45, male, had hypertension history, and low HDL level as risk factors. Keywords: Coronary heart disease PermaLink | Plain Format | Corresponding Author (agus santosa)

146	Biology	ABS-308
Utilization of rice wastewater as an alternative medium for pure brood Shiitake Mushrooms Yati Setiati, Liberty Chaidir, Ariza Saputri Agrotechnology Dept, UIN Sunan Gunung Djati Bandung Abstract Pure breeding multiplication Shitake mushrooms generally use Potato Dekstrose Agar (PDA) media. However, the cost of basic media is relatively higher, so it needs alternative media to multiply pure broodstock for shitake mushrooms to reduce the cost of producing pure broodstock such as rice wastewater. Goal of the research was to find out ability of rice wastewater medium and determine the concentration of the best rice wastewater medium on the growth rate of shitake mushroom mycelium. The study used an experimental method consisting of 11 treatments of rice wastewater concentration 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 100% and Potato Dekstrose Agar (PDA) as the repeated control three replication. The results showed that influence of various concentrations of rice wastewater on the growth rate of shitake mushroom mycelium. The concentration of 100% rice wastewater produces the best growth and quality of mycelium compared to other treatments. Therefore Rice wastewater can be used as an alternative medium to multiply pure broodstock of shitake mushrooms. Keywords: Growth rate, pure culture ,rice wastewater, Shitake mushrooms PermaLink | Plain Format | Corresponding Author (yati setiati)

147	Biology	ABS-564
Factors influencing Jumantik performance in prevention of dengue virus infection in South Denpasar Bali Agus Santosa, IBA Satwika Ekananda Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Jumantik are special officials who perform larva monitoring to reduce the occurrence of DHF. In fact, the high number of DHF cases in Puskesmas II South Denpasar represents a problem regarding the performance of Jumantik. The purpose of this studies were to find out the factors that influent the performance of Jumantik in relation with the occurrence of DHF in the work area of Puskesmas II South Denpasar. This study used descriptive analytic design through cross sectional approach with the total samples of 32 Jumantik in the work area of Puskesmas II South Denpasar. Data were collected by using questionnaires. The obtained data were analyzed by chi square test and logistic regression test. Based on bivariate analysis, there were no significant correlations between characteristic (age, gender, education, tenure, and additional work) and Jumantiks attitudes with the occurrence of DHF indicated by p> 0,05. However, there were significant correlations between Jumantiks ability (p = 0,02), Jumantiks motivation (p = 0,014), award given (p = 0,008) and monitoring evaluation (p = 0,001) with dengue occurrence (p <0, 05). The results of multivariate analysis showed that the variables which influenced the occurrence of DF were ability (p = 0,014) and monitoring and evaluation (p = 0,011) and the most influential were the monitoring and evaluation variables with the value of OR 20,40 which means that if there were no monitoring and evaluation to Jumantik then the risk of DHF occurrence were 20.4 times greater than that in the presence of monitoring and evaluation. The results of this studies showed that the most influential factors of DHF occurrence are the ability of Jumantik and monitoring the evaluation of Jumantik. To reduce the case of dengue, need to be optimized ability jumantik and monitoring evaluation consistently and continuously. Keywords: Jumantik performance; dengue infection; prevention PermaLink | Plain Format | Corresponding Author (agus santosa)

148	Biology	ABS-309
DENGUE SEROTYPING IN ADULT AND PEDIATRIC PATIENTS PRESENTING WITH ACUTE FEBRILE ILLNESS IN WANGAYA HOSPITAL, BALI Asri Lestarini(a), Kartika Sari(a), Dewi Megawati AA(a), Sri Masyeni DAP(a), Suryana K(b), Araniy Fadhilah(c), Yudhaputri FA(c), Artika IM(c), Myint KS(c), Powers AM(d) (a)Medical Faculty, Warmadewa University, Denpasar Bali (b)Wangaya Hospital Denpasar, Bali (c)Eijkman Institute for Molecular Biology, Jakarta (d)Centre for Disease Control and Prevention, Fort Collins, Colorado Abstract Incidence of dengue remains high in tropical and subtropical countries which makes dengue the most common arthropod-borne disease in the region. There is limited data on dengue virus serotype in Bali. This study aimed to assess clinical and virological characteristics of dengue patients in Bali. A cross sectional prospective study was conducted from September 2015 to August 2016. Clinical manifestations were obtained and viral RNA from patient sera were serotyped using conventional RT-PCR. One way Anova was used to compare the clinical manifestation within serotype. Of the 603 patients, 177 cases were confirmed as dengue positive and could be classified into four serotypes. DENV-1, DENV-2, DENV-3, DENV-4. The common clinical manifestations documented were malaise, nausea, anorexia, cephalgia, myalgia, vomiting, athralgia, abdominal pain, retroorbita pain and bleeding. The most prevalent symtoms of cephalgia, bleeding and adominal pain were with DENV-3 infection 63 (66.3%), 11 (11.6%) and 12 (12.6%) respectively. Clinical characteristics such as blood pressure, pulse, temperature, trombocyte, leucocyte, hematocrite and hemoglobin were recorded. Temperature was significantly high in DENV-3 (p 0.030)compared to other serotypes. From all the dengue serotypes that were detected, the most prevalent was DENV-3. There is no significant differences in the clinical manifestations among all serotypes except for high temperature in DENV-3 infection. Keywords: dengue, serotype, clinical manifestation, Bali PermaLink | Plain Format | Corresponding Author (Ni Luh Putu Eka Kartika Sari)

149	Biology	ABS-566
Quality of Malondialdehyde (MDA) Testes of Mice (Mus musculus albinus) After Giving Water Seed Extract and Neem Leaves (Azadirachta indica A. Juss) Ratna Dewi Wulaningsih1, Elsa Lisanti1 1 Universitas Negeri Jakarta (UNJ) Department of Biology, Faculty of Mathematics and Natural Sciences, Kampus A, Gedung Hasjim Asjarie Lt. 9. Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia Abstract One of the compounds contained in neem is flavonoid glycosides, which have anti-fertility effects by reducing hyaluronidase activity, so can prevent penetration of spermatozoa in mice. The mechanism of inhibition of spermatozoa penetration by flavonoids caused by decrease in hyaluronidase activity possible of the influence of gene expression disturbances, so enzyme synthesis decreases. However, the mineral contained in the seeds and leaves of neem can induce oxidative stress in animals, characterized by the increase of Lipid Peroxidation Potential (LPP) in tissues. LPP can be determined by measuring malondialdehyde (MDA) molecules taking the standard thiobarbituric acid (TBA) test. Male mice were divided into nine groups: control (C0), Group t1 - t4 were treated by administering water seeds and leaves extract of neem at doses 0.25 and 0.50 mg/kg bw for 36 days through intraperitoneal injection, without reversibility testing, group T5-T8 were treated by administering water seeds and leaves extract of neem at doses 0.25 and 0.50 mg/kg bw for 36 days followed by reversibility testing for 36 days, with four replications. The results showed the administration of water seed and leaves extract of neem could reduce the MDA levels of mice testis. Conclusion is quality of testicular mice is influenced by oxidative stress conditions in male mice. Keywords: Water, seeds, leaves, MDA, mice, neem, testis PermaLink | Plain Format | Corresponding Author (Ratna Dewi Wulaningsih)

150	Biology	ABS-569
Risk Factors of Stunting Occurrence in Toddlers at Puskesmas Klungkung I 2016/2017 Luh Gede Pradnyawati Faculty of Medicine & Health Sciences of Warmadewa University Abstract Stunting is a growth disorder based on measurement of PB / U or TB / U characterized by a short body state. Toddlers were categorized short if the z-score of the scores is less than -2SD. In Indonesia 29% of children are short category. There are several risk factors that influence stunting including short-term pregnancy, mother’s height, LBW, number of children, age of MP-ASI, exclusive breastfeeding and frequency of infection history with stunting in toddlers. The purpose of this study was to determine the risk factor of stunting in toddlers at Puskesmas Klungkung I. This research is a case-control research with retrospective approach. The sample size used in this study were 35 case samples and 70 control samples, through purposive sampling technique. The data collected in the form of primary data and secondary data. Data were analyzed by bivariate analysis with chi square test and multivariate analysis with multiple logistic regression. Chi square test showed that the risk factors for stunting were LBW, exclusive breastfeeding, age of MP-ASI, frequency of infection history and mothers height. Gestational distance and the number of children were not risk factor for stunting in this study. Multiple logistic regression test showed LBW (OR = 21,98; 95% CI: 2.04-237,69) and mother height (OR = 7,37; 95% CI: 1,94-28,02) became dominant risk factor of stunting incidents in toddlers at Puskesmas Klungkung I. These results indicate that mother’s height and LBW are the risk factors for stunting. Therefore, they need more attention to reduce the incidence of stunting. Keywords: Risk factors, stunting and toddlers PermaLink | Plain Format | Corresponding Author (Luh Gede Pradnyawati)

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