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181	Biology	ABS-622
Antioxidant Capacity of Dillenia sp. Leaf Extract Against DPPH (1,1-Diphenyl-2picryl Hidrazil) Radical Sri Rahayu, Isfi Zahara, Afifah, Krisna Arya Pambudi, Supriyatin Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Jakarta, Indonesia Abstract Simpor (Dillenia sp.) is a plant of the Dilleniaceae family. Simpor (Dillenia sp.) has bioactive compound which is flavonoids that act as antioxidant. The purpose of this study was to measure the antioxidant capacity of the 96% etahnolic simpor leaf extract. CRD (Complete Randomized Design) was applied with 3x5 factors. Extraction of leaf samples done by maceration methode using ethanol 96% with variations in maceration time 24 and 48 hours and series consentration of (10%, 15%, and 20%). The antioxidant capacity assay of simpor leaf series concentration (10, 50, 100, 150, dan 200) ppm using the immersion method of the DPPH (1,1-Diphenyl-2picryl Hidrazil) measure absorption at a wavelength ot 517 nm and compared with the powder of ascorbic acid. The result showed that the ethanol extract has flavonoid qualitatively and quantitatively. Furthermore it also has radical scavenging ability against DPPH (1,1-Diphenyl-2picryl Hidrazil). Keywords: antioxidant capacity, DPPH, leaves simpor, (Dillenia sp.) PermaLink | Plain Format | Corresponding Author (Isfi Zahara)

182	Biology	ABS-878
Biological studies, growth patterns and maturity levels of gonad fish cork (channa striata) living on river and irrigation channel I Putu Suardika, Dewa Gede Semara Edi, I Wayan Arya, Ni Made Darmadi * Water Resources Management Study Program, Faculty of Agriculture, Warmadewa University, Denpasar, Bali *Email: nimadedarmadi[at]gmail.com Abstract This study aims to determine the growth pattern and maturity level of gonad cork fish living in Tukad Yeh Sungi and Subak Baru irrigation channel. This research uses observation method, descriptive and analytic method. Sampling method directly catch fish in the cork by using fishing gear then analytic and described. The samples counted 101 fish samples. Using analytical and descriptive data analysis techniques. The results of the study found the growth pattern of cork fish in negative alometric river while in the irrigation channel for the positive allometric female and the negative alometric male.The maturity level of gonad of cork fish seen directly from the surgical result is influenced by the weight and weight of the fish weight. Keywords: Biological Study, Growth Pattern, Gonad Maturity Level PermaLink | Plain Format | Corresponding Author (Ni Made Darmadi)

183	Biology	ABS-367
Detection of Ligninolytic Capability of Isolated Fungi from Decayed Root and Stem of Oil Palm Tree A. Fijai, Y. Mulyaningsih, N.S. Sepwin & H.B. Aditiya Faculty of Engineering, Sampoerna University Jl. Pasar Raya Minggu No 16, Jakarta Selatan, Indonesia Abstract Lignin is difficult to be decomposed due to its complicated carbon bonding. Previous studies have discovered that ligninolytic enzymes effectively degrade lignin waste. Numerous microorganism such as white rot fungi have been taken into account to accelerate the decomposition process of lignin. The aim of this study is to detect the capability of the fungi which was isolated from decayed root and stem of oil palm tree. Qualitative test using guaiacol (0.02%) was performed to observe the ligninolytic activity of the fungal isolates. The results of the test indicated that two out of ten fungal isolates were able to produce ligninolytic enzyme. Successful of this study could be used as baseline information of potential fungi which are able to degrade lignin waste in environment. Keywords: lignin, lignin waste, ligninolytic activity, white rot fungi PermaLink | Plain Format | Corresponding Author (Yhana Mulyaningsih)

184	Biology	ABS-623
CORRELATION OF COGNITIVE SCORES WITH CD4 AMONG HIV CHILD IN PAEDIATRIC POLYCLINIC, SANGLAH HOSPITAL, DENPASAR, BALI Putu indah Budi Apsari, I Nyoman Supadma faculty of medicine and health sciences, warmadewa University Abstract Cognitive, motoric and language aspect of HIV children is an important issue that affect developmental milestone and quality of life. The effect of HIV infection on cognitive function remains unclear. This study aimed to assess correlation of cognitive scores with CD4 among HIV child in Paediatric polyclinic Sanglah Hospital, Bali. This cross sectional study recruited 68 HIV child age 0-36 months old as participants. Cognitive score was assessed using CAT/CLAMS scores, HIV status was obtained from medical record. The result showed average of CD4 among subject was 29.85 cells/mm3, there was positive correlation between CAT scores with CD4 count (r = 0.33, p = 0.006) and also between CLAMS scores with CD4 count (r = 0.307, p = 0.01). It can be concluded CD4 count correlated with cognitive scores on HIV child. Keywords: HIV child, cognitive score, CAT/CLAMS PermaLink | Plain Format | Corresponding Author (Putu Indah Budi Apsari)

185	Biology	ABS-1135
Endemic species: morphometric differences between male and female of black-winged myna (Acridotheres melanopterus) Renaldi Ednin Vernia1, Retno Lestari1, Abinawanto1, Nurul Winarni2, Agung Sedayu3, and Anom Bowolaksono1* 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, West Java, Indonesia. 2Research Center for Climate Change, Universitas Indonesia, Depok, West Java, Indonesia. 3Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jalan Rawamangun Muka No. 1, 13220 East Jakarta, Indonesia. Abstract Black-winged myna (Acridotheres melanopterus) is an endemic species from Indonesia. Nowadays, the bird can be found only in Java and Bali islands, and it is important to make sure that the breeding centre is effectively breeding the bird to help the decreasing population wilderness. There is no difference between male and female based on the morphological characteristic. It can be a problem for the breeders to identify the sex when they have to pair the bird. The aim of this research is to identify the differences between male and female black-winged myna based on morphometric characteristic. There are weight, length, width, circumference, and the spread of the wings. The data is processed using principal component analysis (PCA) on the SPSS 20 program. The samples are 71 black-winged myna with minimum two years of age from breeding facilities in Java and Bali islands. Based on the research, morphometric characteristic that distinguishes male and female bird mostly is their wingspan and their weight. Morphometric characteristics of the female myna are smaller than the male. This result is essential for breeders to minimize the risk of pairing the same sex bird. Keywords: Endemic species; morphometric; black-winged myna PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

186	Biology	ABS-880
Nutrient fulfilling status for bali cows in Bali in season differences I Dewa Nyoman Sudita Faculty of Agriculture Warmadewa University Denpasar Bali idnsudita[at]gmail.com Abstract This study aims to determine the amount of nutrients given in feed for bali cows in Bali in different seasons. The study used a survey method through observations on 27 livestock groups in Bali by measuring the amount of feed given, diversity of feed ingredients, and sampling of feed ingredients for analysis. Measurements were made three times, namely in the rainy season (February-March), mid-dry season (June-July), and the peak of the dry season (September-October). The results showed a decrease in the type and diversity of feed ingredients in the ration, while the amount of ration given (kg/day of fresh weight) was almost the same (33.17; 33.15; and 32.82 kg). The total protein available in the ration was decreased in the rations given, namely: 706.52 g (rainy season), 648.65 g (mid-dry season), and 611.37 g (peak dry season). While the total energy tends to increase according to successive seasonal changes: 12,650.70; 13,820.18; and 13,974.21 kcal ME. From this study it can be concluded that although there is a decrease in the type and diversity of feed ingredients in the rations given to bali cows according to the season in Bali, but the amount of protein and energy above the basic livelihood needs of livestock. Keywords: Fulfillment of protein and energy, bali cows PermaLink | Plain Format | Corresponding Author (I Dewa Nyoman Sudita)

187	Biology	ABS-1136
Luteinizing hormone (LH) supress apoptosis in bovine luteal cells Anom Bowolaksono1*, Anantya Pustimbara1, Retno Lestari1, Abinawanto1, and Rusdi2 1 Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok 16424, West Java, Indonesia 2 Department of Biology, Universitas Negeri Jakarta, Jl. Rawamangun Muka, East Jakarta, 13220, Indonesia Abstract Regression of the corpus luteum (CL) is characterized by loss of the capacity to synthesize progesterone (P4) and tissue degradation by apoptosis (functional and structural luteolysis). Luteinizing hormone (LH) is one of the most potent stimulators in progesterone production by corpus luteum (CL) of most mammals, including cow. This research hypothesized that LH suppresses apoptosis of bovine luteal steroidogenic cells (LSC). We examined the effect of LH on cell death such as cell viability and DNA fragmentation factors which related with apoptosis. Cultured bovine LSC obtained from the CL at the mid stage (Days 8–12 of the cycle) were treated for 24 h with recombinant human tumor necrosis factor alpha (TNF; 2.9 nM) and recombinant bovine interferon gamma (IFNG; 2.5 nM) in the presence or absence of LH (0.34 nM). LH reduced the levels of cell death in the LSC induced by TNF and IFNG (P<0.05). Furthermore, DNA fragmentation induced by TNF and IFNG was observed to be suppressed by LH. In conclusion, LH affects viability of luteal cells as an anti-apoptotic factor in bovine CL by increasing cell viability and suppressed DNA fragmentation. This suggests that LH plays luteoprotective roles in bovine CL by suppressing apoptosis of LSC. Keywords: Luteinizing hormone; supress apoptosis; bovine luteal cells PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

188	Biology	ABS-1137
Ingestion of microplastics by anchovies from Talisayan harbor, East Kalimantan, Indonesia Endar W. Ningrum1, Mufti P. Patria1 and Agung Sedayu2 1Department of Biology, Faculty of Mathematics and Natural Science, University of Indonesia, Depok, IDN 2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Jakarta Timur, Indonesia, 13220 Abstract Microplastics (MPs) is the most contamination problem on the marine environment. Small pelagic fish like anchovies are one of the organism which exposured by microplastics, yet reported contamination on Indonesian anchovies is lacking. Here, the investigation result from anchovies digestive tract (Stolephorus spp.) sampled (n = 15). The total microplastics contamination from Talisayan harbor, East Kalimantan is 366 ± 3,51 particles/individual. Kind of microplastics size range detected as categories: < 20 µm, 20-50 µm, 50-500 µm, 500-1000 µm, and >1000 µm. Most of microplastics shapes from Talisayan harbour are microfilm (50%) and microfiber (29,59%). The type of polymers are confirmed by Fourier transform infrared (FTIR) spectroscopy as polypropylene, high-density polyethylene and foamed polystyrene nylon. Our findings reported that spesific size range (50-500 µm) is the most majority of microplastics size range which are ingested by anchovies from Talisayan harbor. The microplastics are more dangerous if another contamination found together on it. On this investigation, the mercury contamination also tested and the result showed as not detected mercury contaminant. The anchovies are had the economic value and also small pelagic fish which are play as prey for the other big fish. The exposured are possible to the human by contaminated seafood diet. Keywords: Ingestion; microplastics; anchovies PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

189	Biology	ABS-882
GENETIC DIVERSITY OF THREE SUNU GROUPERS SPECIES (Plectropomus spp) Sang Ayu Made Putri Suryani (a), I Wayan Arya (b) (a) Aquatic Resources management department University of Warmadewa, Denpasar, Bali Email: suryanip[at]rocketmail.com (b) Aquatic Resources management department University of Warmadewa, Denpasar, Bali Abstract Fish seed quality may be decreased as a result of inbreeding that is a marriage between two individuals who have the same genetic trait. Improved seed quality can be done with the genetic approach to parent selection and crossbreed. Quality seeds which are taken from natural populations are determined by the genetic diversity of a population. Inbreeding can lower the quality of seed because it can increase the number of homozygosity and heterozygosity otherwise decrease the number.This study aims to determine differences in genetic variation three species of grouper groupers (Plectropomus maculatus, Plectropomus leopardus, and Plectropomus oligacanthus) and know the kinship of the three species of grouper. While the benefits of this research can provide information on genetic diversity in natural coral trout grouper, expected inbreeding can be avoided. Muscle and liver tissue was used as a sample for electrophoresis analysis with twelve kinds of enzymes are aspartate aminotransferase (AAT), Alcohol dehydrogenase (ADH), Estrase (EST), -Gliserofosfat dehydrogenase (-GPD), Glukofosfat isomerase (GPI), Isocitrate dehydrogenase (IDH), Lactate dehydrogenase (EST), Malic dehydrogenase (MDH), Malic Enzyme (ME), 6-phosphogluconate dehydrogenase (6- PGD), phosphoglucomutase (PGM), and sarcoplasmic enzymes (SP). Three polymorphic loci in Plectropomus oligacanthus species are 6-PGD, GPI-2, and PGM. In Plectropomus maculatus, and Plectropomus leopardus all monomorphic loci. The three polymorphic loci on Plectropomus oligacanthus meet the Hardy-Weinberg balance with X2 value for 6-PGD value is 0.123 for GPI-2 is 0.028. And PGM is 0.028. Plectropomus oligacanthus has a polymorphism level of 0.188, the total number of the focusing allele is 1,154, and the average heterozygosity is 0.023. The genetic distance between the sepsis population based on twelve enzymes showed between Plectropomus maculatus, and Plectropomus leopardus had a spacing of 0.134, between Plectropomus leopardus and Plectropomus oligacanthus of genetic distance 0.196 and between Plectropomus maculatus and Plectropomus oligacanthus the genetic distance was 0.200. The larger the genetic distance between the species, the kinship relationship will be farther away. The distant kinship is owned by Plectropomus oligacanthus. Keywords: Genetic diversity, Grouper species, Plectropomus sp, PermaLink | Plain Format | Corresponding Author (Sang Ayu Made Putri Suryani)

190	Biology	ABS-1138
Microplastic Abundance in the Water, Seagrass, and Sea Hare Dolabella auricularia in Pramuka Island, Seribu Islands, Jakarta Bay, Indonesia Vincentia Priscilla1, Mufti Petala Patria1, a), Agung Sedayu2 1Department of Biology, Faculty of Mathematics and Natural Sciences., Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia 2Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Rawamangun, Jakarta Timur, Indonesia, 13220 Abstract This research focused on the amount of abundance of microplastics in the sea hare Dolabella auricularia as well as the seagrass fields along the southern coast of Pramuka Island. Sampling of 8 individuals of Dolabella auricularia along with seagrass Cymodocea rotundata leafblades was done at the southern coast of Pramuka Island, after which the samples were preserved and brought to a laboratorium in Depok for microplastic analysis. The sea hares’ digestive tracts were extracted and dissolved in strong nitric acid. A 1 cm2 portion of a seagrass leaf blade was cut for observation. Prepared samples were observed under a monocular microscope and further analysis was done. Microplastic fibers and film particles were found in highest abundance within the digestive tracts of each sample of Dolabella auricularia as well as on the seagrass surface where the sea hare obtains its algae from, with fragment particles found in much lower amounts. Overall, number of microplastics was found between 40.1 to 73.7 particles/g weight of sea hare digestive tract and the estimated amount of microplastic found at seagrass leafblade was 185 particles/cm2. Results provide evidence that microplastics in the ocean brought by water currents could adsorb on to algae through which it enters the food chain as it is consumed by marine biota. Keywords: Pramuka Island, Dolabella auricularia, sea hare, Microplastic, seagrass PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

191	Biology	ABS-888
LONGITUDINAL DISTRIBUTION AND POPULATION STRUCTURE Rasbora lateristriata Bleeker, 1854 (osteichthyes: Cyprinidae) IN SUNGI RIVER Sang Ayu Made Putri Suryani (a) I Wayan Arya (b) I Made Kawan (c) (a) Aquatic Resources Management, Warmadewa University suryanip[at]rocketmail.com (b) Aquatic Resources Management, Warmadewa University (c) Aquatic Resources Management, Warmadewa University Abstract Genus Rasbora is a freshwater fish belonging to the Cyprinidae familia distributed covering the Asian region, especially in Southeast Asia. There are 90 species of fish Rasbora scattered around the world where 56 species distributed in Indonesia and 3 species are endemic to Indonesia. Rasbora fish used as an alternative source of protein because protein is high at 33.4 g / 100 g. Rasbora higher protein content than the protein content of carp and milkfish, Rasbora also contain high fluorine and contains omega3. This study aimed to describe the longitudinal distribution and population structure in the basin Rasbora lateristriata Sungi, Tabanan. Analysis of population structure based on weight and the total length, determine the interaction between physical and chemical factors with an abundance of Rasbora lateristriata. This study used a survey method with purposive random sampling technique to collect the sample. This study uses three stations by topography and environment, namely the upstream, midstream and downstream. Rasbora species diversity and abundance will be calculated by cluster analysis, longitudinal distribution and population structure will be explained descriptively. The interaction between physical factors with an abundance of Rasbora will be analyzed by principal component analysis. In the current study found that two species Rasbora Rasbora lateristriata and Rasbora sp. with 132 individual species of Rasbora lateristriata and distributed longitudinally along the river Sungi on the middle and downstream. Lateristriata Rasbora weight ranging from 1, 3 to 6 grams, the total length ranges from 4 to 7.5 cm. Lateristriata Rasbora distribution is affected by the DO, temperature, TSS and BOD. The existence of Rasbora lateristriata influenced by light intensity and depth Keywords: Rasbora lateristriata, longitudinal distribution, physical-chemical, Sungi River PermaLink | Plain Format | Corresponding Author (Sang Ayu Made Putri Suryani)

192	Biology	ABS-892
Pregnancy Diagnostic for Lowland Anoa (Bulbalus depressicornis): Study of Behavior and Importance of Ultrasonography Accuracy on Wild Fauna Preservation Anita Mayasari (1), Luthfiralda Sjahfirdi (1), Abinawanto (1), Retno Lestari (1), Astari Dwiranti (1), Adven Tri Joy Simamora (2), Rahma Suryaningsih (2), Ady Suryawan (2), Ade Suryanda (3), Riser Fahdiran (3), Anantya Pustimbara (1), and Anom Bowolaksono (1*) 1) Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, 16424 Depok, West Java, Indonesia 2) Environment and Forestry Research and Development Institute of Manado, Adipura road, Kima Atas, Mapanget, 95259 Manado, North Sulawesi, Indonesia 3) Faculty of Mathematics and Natural Sciences, State University of Jakarta, Rawamangun Muka road No. 1, 13220 East Jakarta, Indonesia Abstract Early pregnancy diagnostic is very important to support the attempt of fauna preservation. Lowland anoa is an endangered endemic species for Sulawesi Island and Buton. This species has natural wild characteristic and aggressive, intensified with silent heat effect which often occurs in this lowland anoa, makes it hard to detect its estrous cycle and pregnancy. The research is aimed to observe and morphology changing in pregnancy, and to find out diagnostic accuracy in pregnancy of lowland anoa using abdominal ultrasonography. Ultrasonography abdominal checking was used with 95% alcohol as transduction medium. Ultrasonography method was performed before mating and at day-21, day-191, and day-218 post natural mating using one dwarf female buffalo of 9 years old. Result showed that lowland anoa act was changing in 2nd after mating until 6th month before giving birth. Their morphological changing had shown in 3rd month, 6th month and 8th. In the 9th month the milk udder has enlarged. Based on the result of ultrasonography on day-21, it is very difficult to find the gestational sac. However, on day-191, the fetus spine, head, heart and lungs are clearly visible. Keywords: Lowland Anoa; Pregnancy; Behavior; Ultrasonography PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

193	Biology	ABS-893
Effect of Age and Body Mass Index on Oocyte Maturation Rate and Blastocysts Development Diana Novia (1), Anantya Pustimbara (1), Binarwan Halim (2,3), Abinawanto (1), Retno Lestari (1), Rusdi (4), Riser Fahdiran (4) and Anom Bowolaksono (1*) 1) Department of Biology, Faculty of Mathematics and Natural Sciences of Universitas Indonesia, Depok, West Java, Indonesia 2) Division of Reproductive, Endocrinology and Infertility, Department of Obstetrics and Gynaecology, Faculty of Medicine University of Sumatera Utara, Haji Adam Malik General Hospital, Medan, Indonesia 3) Halim Fertility Centre, Stella Maris Women and Children Hospital, Medan, Indonesia 4) Faculty of Mathematics and Science, Universitas Negeri Jakarta, East Jakarta, Indonesia Abstract Oocyte maturation rate is an important factor in determining a good fertilization and embryo development rate with in vitro fertilization. Embryos that can develop until blastocyst stage have been proven to have a higher success rate than cleavage stage embryos. The aim of this study is to find out effect of age and body mass index (BMI) in oocyte maturation rate and the successfulness of blastocyst development. In this study, 158 infertile women within age range of 22-47 who underwent IVF-ICSI program in Halim Fertility Center were analyzed. The IVF-ICSI outcomes assessed were number of oocytes retrieved (OR), number of matured oocytes (MO), fertilization rates (FR), cleave rates (CR), the blastocyst rates (BR), and the pregnancy rates (PR). All data were divided into three groups based on WHO BMI classification (normal range, overweight and obese) and four groups based on age classification (up to 30, 31-35, 36-40, below 41). These data were analyzed with student T test method and chi-square. In result, our study concludes that the body mass index shows a negative effect of oocyte maturation process and blastocyst development. However, age had an adverse impact on number of oocyte retrieved, number of matured oocyte, and blastocyst rates. Keywords: Oocyte Maturation; Blastocyst; Age; Body Mass Index. PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

194	Biology	ABS-894
Composition and Diversity of Fish Species in Mangrove Ecosystem at Muara Binuangeun, Lebak, Banten Noer Kholis (1), Titi Soedjiarti (1), Riser Fahdiran (2), Mufti Petala Patria (1*) (1) Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Western Java, Indonesia, 16424 (2) Faculty of Mathematics and Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia, 13220 Abstract Research of composition and diversity of fish species in mangrove ecosystem at Muara Binuangeun, Lebak, Banten, had been conducted at May and November 2015. Catch per Unit of Effort (CPUE) was used as a method with push net and boat net as fishing gear. Fishing was conducted during low tide. Collected samples were preserved with 10 % Formalin Solution and then being identified at laboratory. In total, 106 fishes were collected from 11 families and 28 species. Istigobius ornatus was the most relative abundant species (19,81 %) and the highest relative frequency too. Diversity index value of mangrove ecosystem was 2,846. The composition of collected fish showed several migrant species from seagrass bed and coral reef ecosystem. Keywords: Fish Composition, Diversity, Mangrove, CPUE, Istigobius ornatus. PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

195	Biology	ABS-639
Phytochemical Screening and Proximate Analysis of Soybean (Glycine Max) Variety Gamasugen 1 and Gamasugen 2 Derived From Gamma Rays Irradiation Elsa Lisanti1, Arwin2 1 Universitas Negeri Jakarta (UNJ) Department of Biology, Faculty of Mathematics and Natural Sciences, Kampus A, Gedung Hasjim Asjarie Lt. 9. Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia 2 Center Application Isotopes and Radiation (CAIR), National Nuclear Energy Agency of Indonesia, Jl. Raya Lebak Bulus No. 49, Jakarta Selatan 12440, Indonesia Abstract Soybean is very widely used in Indonesia, especially as a raw material for processed foods such as tofu and tempeh. This study aims to analyze the phytochemical content and proximate of variety irradiation soybean seeds Gamasugen 1 and Gamasugen 2. Phytochemical and proximate analysis on the seeds of soybean were carried out with quantitative and qualitative analysis. Proximate analysis analyzed included water content (oven method), ash content (furnace method), fat content (Soxhlet method), and crude fiber content (acid alkali digestion method). The results of phytochemical testing shows that both soybeans (Gamasugen 1 and Gamasugen 2) contain flavonoid as quercetin, alkaloid, saponins, phenolic, triterpenoid and glycoside. The proximate analysis results showed that Gamasugen 1 soybeans had a moisture content of 8.22%, lower than Gamasugen 2 which was 11.67%. Gamasugen 1 soybean content is 4.61%, lower than Gamasugen 2 which is 4.86%. Gamasugen 1 soybeans have a fat content of 14.50%, lower than Gamasugen 2 which is 15.51%. Gamasugen 1 soybeans have fiber content of 14.56%, higher than Gamasugen 2 soybeans which is 9.96%. The conclusion shows the highest proximate analysis of soybean is found in fat content (Gamasugen 2) which is 15.51% and the lowest level is found in ash content (Gamasugen 1) which is 4.61%. Keywords: Gamasugen, irradiation, phytochemical, proxymate, soybeans PermaLink | Plain Format | Corresponding Author (Elsa Lisanti)

196	Biology	ABS-895
FISH COMMUNITY STRUCTURE IN MANGROVE FOREST OF PANJANG ISLAND, BANTEN BAY Meilisha Putri Pertiwi (1), Noer Kholis (1), Riser Fahdiran (2), and Mufti Petala Patria (1) (1) Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Western Java, Indonesia, 16424 (2) Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Jakarta, Indonesia, 13220 Abstract Research of fish community structure in Panjang Island, Banten Bay has been conducted from October to December 2014 while spring tide. The aim of the research was knowing the fish biodiversity and fish community structure. The fishing method was used push net manually active. The location were at Station 1 (West) with sandy-mud and gravelled substrate and Station 2 (North) with mud-reef substrate. In total, 1770 species were captured (14 families, 16 genera, and 21 species), the most large number were at Station 1 and in November (1.213 and 749 individus). Both stations had a low species diversity index (H’) value (0,705 and 0,807). It supported by a low eveness index or distribution of individuals among species (E) value. Its high dominance index (D) value was due to the predominance of Ambassis gymnocephalus-Chandidae (908), Ambassis buruensis-Chandidae (297), Oryzias javanicus-Adrianichthyidae (265), Chelon sp.-Mugilidae (123), and Gerres sp.-Gerreidae (22) in large numbers. Keywords: Chandidae; Mangrove; Panjang Island; Push Net; Fish Community PermaLink | Plain Format | Corresponding Author (Riser Fahdiran)

197	Biology	ABS-644
THE PERFORMANCES OF GROWTH, BODY WEIGHT, AND MILK YIELD HOLSTEIN FRISIEN (FH) DAIRY COW IN DIFFERENT AGRICULTURAL ECOSYSTEM T Kusmayadi1, M Hasan2, U H Tanuwiria2, E Herawati1 1Faculty of Agriculture, Universitas Garut, Indonesia 2Faculty of Animal Husbandry, Universitas Padjajaran, Indonesia Corespondence author: Email : tendy84[at]uniga.ac.id Abstract This research was about the performance of growth, body weight and milk production of Friesian Holland dairy cow in different agricural ecosystems inIndonesia.The objective of research was to study growth performance data, body weight and FH dairy cow milk production in Indonesia. The research was carried out in the three agroecosystems, Lebakjaya Village (rainfed rice field and dry land/AES DL-Rainfed), Karangpawitan District; Cintanagara village (irrigated rice field and dry lands/AES DL-IRF), Cigedug District; and Pamalayan village (dryland and tropical forest/AES DL-Forest), Bayongbong District.The method of the research was survey method, with the technique of determining farmers proportionally, and sampling farmers and their livestock by random sampling method. The data was processed using SPSS 22.0 for the simple descriptive analysis, and then tested statistically used T-Test.The results it was concluded that growth, body weight and milk yield of dairy cows were influenced by agroecosystems, in the dry land agroecosystems and located on or adjacent to forests (AES DL-Forest), productivity of dairy cows was better than irrigated and dry land with wide balanced rice agroecosystems (DL-IRF) and rainfed and dry land agroecosystems (AES DL-Rainfed). Keywords: FH Dairy cow, body weight, milk yield, agroecosystem PermaLink | Plain Format | Corresponding Author (Tendy Kusmayadi)

198	Biology	ABS-389
The Effect of B-Carotene (C 40 H 56 ) in Carrots (Daucus carrota) as a Natural Additive Substance for Wet Noodle Durability A Rohayat, R Nurkhotimah, C Ardiana,, S Mulyaningsih, D Hernawati Biology Department, Institut Pendidikan Indonesia Abstract Abstract Additives are chemical compounds added to preserve food. There are two types of additives; natural and artificial additives. The durability of wet noodles is relatively lower compared to other staple food. There are various methods to preserve wet noodles. One of which is the addition of natural additives. Natural additives are chosen because they are safer than artificial additives which are more precarious to human health. The natural additives used are carrots because they contain B-carotene compounds functioning as antioxidants that reduce and or deactivate free radical attacks and ROS (Reactive Oxygen Species) on food caused by microorganisms. These processes increase the durability of wet noodles. The research aimed to determine the effect of B-carotene content in carrots as a natural additive for wet noodle preservation. The method used was an experiment with B-carotene concentration variations in carrots; 0%, 20%, 40%, 60%, and 80%. From the results, it was found that at the 60% concentration of B-carotene, the durability of wet noodles reached the maximum 4 day. This fact showed that the addition of high concentration B-carotene will affect the durability of wet noodles. And carrots can be used as natural additives in preserving wet noodles. Keywords: B-carotene, Additives, durability, wet noodles PermaLink | Plain Format | Corresponding Author (Asep Rohayat)

199	Biology	ABS-391
Interleukin 8 levels in patients with dengue fever Ni Putu Diah Witari (a*), Asri Lestarini (a), Dewa Ayu Putri Sri Masyeni (a), I Gede Agus Sastrawan (b), I Gusti Ayu Asih Ratnadi (b) a) Faculty of Medicine and Health Sciences, University of Warmadewa, Denpasar, Bali *diahwitari[at]gmail.com b) Tabanan General Hospital, Bali Abstract Indonesia as one of the tropical countries has a high prevalence of dengue. Pathophysiology of dengue fever is through the role of the immune system, one of which is the role of cytokines. Interleukin 8 (IL8) is one of the pro-inflammatory cytokines produced by macrophage cells in the immune response. Several studies have linked interleukin 8 levels with dengue infection. The purpose of this study was to determine interleukin 8 levels in patients with dengue fever. The research design used was a cross-sectional study. Serum samples of 50 samples were collected and then examined for interleukin 8 levels using the ELISA method. The results of this study, more research subjects diagnosed with dengue fever as much as 90% (45 samples) and 10% (5 samples) dengue hemorrhagic fever. Through the Mann-Whitney test analysis, IL8 levels are expressed in the median (minimum-maximum). Levels of IL8 in dengue fever 19.76 (13,70-89,62) and 25,15 (15,74-25,87) in dengue hemorrhagic fever. The conclusion is that interleukin 8 levels were not statistically different between patients with dengue fever and dengue hemorrhagic fever. Keywords: Interleukin 8, dengue fever, dengue hemorrhagic fever. PermaLink | Plain Format | Corresponding Author (ni putu diah witari)

200	Biology	ABS-392
Correlation between Body Mass Index and Flat Foot in elementary school at Denpasar, Bali Komang Trisna Sumadewi (a*), Sri Ratna Dewi (a) a) Faculty of Medicine and Health Science, Warmadewa University Jalan Terompong no 24, Denpasar 80235, Indonesia *drtriscel[at]gmail.com Abstract Flat foot or also known as pes planus is a condition in which the inner or medial arches are reduced or absent so that the sole of the foot will touch the ground. This situation can be physiological which is called a flexible flat foot or pathological which is called a rigid flat foot. Flat foot is one of the most common conditions encountered by pediatrics, which affects around 20% to 30% of the worlds population. With increasing age, the prevalence of flat foot will decrease. Flat foot causes foot instability as a bodybuilder. This study aims to look at the relationship between Body Mass Index (BMI) and the incidence of flat foot in grade 1 and 2 children at SD 5 Sumerta, Denpasar. This study is an analytical study using a cross-sectional approach with a sample of 40 conducted at SD 5 Sumerta Denpasar. The data obtained was carried out a descriptive analysis and correlation test. From this study, it was found that BMI had a correlation with Flat foot (p=0.001; r=0.695). Confidence Interval (CI) 95%. In conclusion, the Body Mass Index has a significant positive correlation with flat foot prevalence. Keywords: Flat Foot, Body Mass Index, Elementary School Students PermaLink | Plain Format | Corresponding Author (Komang Trisna Sumadewi)

201	Biology	ABS-649
The Association Between Toilet Training, Physical and Family History with Incident of Enuresis in Children at North Denpasar Asri Lestarini (a*), Putu Satya Prameswari (a) (a) Faculty of Medicine and Health Sciences, Warmadewa University Jalan Terompong no 24 Denpasar, 80235, Indonesia *asrilestarini[at]gmail.com Abstract Enuresis, also known as bed-wetting is a condition where the children at the age of 5 or more urinate on the bed or on their clothes, and it happened often about at least twice a week for 3 months in a row. This research is dedicated to studying the relation between toilet training, physical and family history with the incident of enuresis in children between 5 and 6 years old. A cross-sectional study was conducted in North Denpasar District. The samples were 105 subjects whose study at kindergarten and their parent. Data of the risk factor of enuresis were collected by using questioner and then analyzed by chi-square and Fisher exact test. The result of this research are children who had parents with enuresis history (PR = 3,720), children who had siblings with enuresis history (PR = 4,950), breastfeeding history (PR = 1,920), duration of breastfeeding (PR = 2,133), toilet training (PR = 5,497), and bad physiological history (PR = 1,915) were related to incident of enuresis in children with p-value <0,05. Based on the result, parents, educators, or medical personnel may prevent the incident of enuresis by avoiding the risk factor of enuresis. Keywords: enuresis, family history, toilet training PermaLink | Plain Format | Corresponding Author (Asri Lestarini)

202	Biology	ABS-394
Difference Between Inhibitory Power Between Extract of Guava Leaves and Salam Leaves Against Escherichia coli Bacterial Growth Ni Putu Diah Witari (a*), Teresita Marselina Nahak (a), Wayan Semadha (a) a) Faculty of Medicine and Health Sciences, University of Warmadewa, Denpasar, Bali *)diahwitari[at]gmail.com Abstract Escherichia coli is a Gram-negative bacterium that has a natural habitat in the human digestive tract. This bacterium besides being a normal flora can also be pathogenic. Escherichia coli is the most common cause of diarrhea. Guava leaves and bay leaves contain essential oils, tannins, flavonoids, and saponins which have antimicrobial effects. The purpose of this study was to determine the difference in the effectiveness of inhibition between ethanol extract of guava leaves and bay leaves on the growth of Escherichia coli bacteria. This type of research is an experimental laboratory. The inhibitory test was carried out by measuring the clear zone around the paper disc using callipers. The results of data analysis using one way ANOVA showed that there were significant differences between groups of extracts with F output = 49.83 and p = 0.00 (p≤ 0,05). Guava leaf extract concentration of 10%, 30% was significantly different from bay leaf extract concentration of 10%, 20%, 30%, with a p-value ≤ 0.05. The conclusion of this study is Ethanol extract of guava leaves has a higher inhibitory ability than the ethanol extract of bay leaves. Keywords: Escherichia coli, guava leaves, bay leaves PermaLink | Plain Format | Corresponding Author (ni putu diah witari)

203	Biology	ABS-395
PREMINARY TEST OF CYTOTOXIC ACTIVITY OF SOME PLANTS OF THE ANNONACEAE FAMILY USING THE BSLT METHOD (BRINE SHIRIMP LETHALITY TEST) Farid Perdana1*, Ria Mariani1, Vera Setiawati1 1. Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Univesitas Garut Jl. Jati No. 42 B Tarogong Garut, Jawa Barat *farid[at]uniga.ac.id Abstract Compounds in plants can inhibit or even kill cancer cells so that they have the potential as anticancer agents. One of the potential plants as an anticancer agent is the annonaceae family. This reserch as a screening method using Brine Shrimp Lethality Test is to find out the cytotoxic activity of soursop leaves, srikaya leaves, and nona leaves. The result of the preliminary test of cytotoxic activity of ethanol extract of soursop (Annona muricata L), srikaya (Annona squamosa L) and nona (Annona reticulata L)leaves showed cytotoxic activity wilth LC50, respectively 30,90; 38,01; dan 46,77 ppm. Based on the results of the study all extracts have cytotoxic activity. The highest cytotoxic value was shown by soursop leaf ethanol extract with LC50 value of 30,90 ppm. Keywords: BSLT, Annonaceae Family, Cytotoxic PermaLink | Plain Format | Corresponding Author (Farid Perdana)

204	Biology	ABS-908
Application of Killer Yeast Derived from Phylloplane Bintaro Leaf as a Biocontrol Agent for Destructive Molds in Postharvest Fruits on Malang Apple Dalia Sukmawati1,2, Andisa Shabrina1, Iman Hidayat3, * Hesham El Enshasy4,5 1) Biology Department, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia 2) Universitas Negeri Culture Collection, 9th Floor Hasyim Ashari Building, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta, Indonesia 3) Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI), Indonesia. 4) Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), 81130 UTM, Skudai, Malaysia 5) Department of Bioprocess and Polymer Engineering, School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, 81310, Skudai, Johor, Malaysia Abstract Mold is one of causal agents of postharvest diseases in apples. These include Aspergillus sp., Penicilium expansum, Botrytis cinerea, and Venturia sp. Fungicide application in controlling postharvest diseases causing several problems such as fungal resistance, environmental pollution and health. Therefore, it is important to seek alternative solution which is safe to human and environment, such as biocontrol. The aims of this study were to examine biocontrol activity of yeast isolated from bintaro leaves in inhibiting the growth of fungal postharvest diseases in apples and to determine the identity of these yeasts by using molecular phylogenetic analysis of nucleotide sequencing from the ITS rDNA region. Samples of apples were collected from Perumnas Traditional Market, Klender, East Jakarta. Isolation was done by using direct isolation techniques. A total of 18 fungal isolates have been isolated from symptomatic apples. Pathogenicity assay showed that A. brasiliensis sensu lato strain A1 (KP value 100%; KeP 50%) and A. flavus sensu lato strain A17 (KP value 100%; KeP 31.25%) exhibit highest activity in deleterious apple fruits. Rhodotorula mucilaginosa strain T1 and two isolates of Aureobasidium c.f. pullulans (strain T3 and T4) showed highest antagonism activity against the fungal pathogens. Biocontrol assay showed that yeast isolates were capable to reduce the growth of A. brasiliensis and isolates T1 and T3 were capable to reduce the growth of A. flavus. Keywords: Apple, Aureobasidium pullulans, biocontrol, fungi, Rhodotorula mucilaginosa PermaLink | Plain Format | Corresponding Author (Dalia Sukmawati)

205	Biology	ABS-653
Isolation of Potential Proteolytic Bacteria from Hospital Wastewater SR Dewi*, AAAL Paramasatiari, NW Rusni Faculty of Medicine and Health Science, Universitas Warmadewa, Bali-Indonesia Abstract Wastewater needs to be processed before being disposed. Generally the wastewater that produced by a hospital contains a lot of bacteria, viruses, chemical compounds, and drugs which can be harmful for the health of the surrounding community. For this reason, it is necessary to process hospital wastewater by using effective and efficient wastewater treatment technology. One of the processes of wastewater treatment is biological processing that use microorganisms. Aim of this study is to isolate microbial that produce proteolytic capabilities that are potential in treating hospital wastewater. This research is a descriptive study with cross sectional design. Samples were taken from the Waste Water Treatment Plant of Kasih Ibu General Hospital in Denpasar. The screening of the proteolytic bacteria was done by growing the isolated culture on skim milk agar. The colonies that produce clear zone were identified as proteolytic bacteria. In this study a total of 43 isolates were obtained and two isolates showed their proteolytic activity. Both isolates are Gram positive cocci and Gram negative bacilli. Keywords: isolation; bacteria; hospital; wastewater; proteolytic PermaLink | Plain Format | Corresponding Author (Sri Ratna Dewi)

206	Biology	ABS-398
Comparison of Lung Function Before and After Exercise in Normal and Obesity of Warmadewa University Students, Denpasar, Bali Komang Trisna Sumadewi (a*), I Gusti Ngurah Bagus Bayu Pramana (a), I Gusti Ngurah Putu Sana (a) a) Faculty of Medicine and Health Science, Warmadewa University Jalan Terompong no 24, Denpasar 80235, Indonesia *drtriscel[at]gmail.com Abstract Obesity is one of the greatest factors causing health problems. Many studies have shown a significant association between obesity and lung disorders in the form of obstructive sleep apnea (OSA) and asthma. The purpose of this study is to know the ratio of lung function before and after exercise in the normal and obesity group. The research design used was quasi experimental with pretest and posttest design, using Pearson ChiSquare hypothesis test, Wilcoxon Sign Rank Test and Mann Whitney U Test. There are 60 samples divided into normal and obese with an average age of 19.47, consisting of men and women in each of 30 people. The result of this research is in the normal group average of delta value of FEV1 equal to 0,13, FVC equal to 0,10, and lung function equal to 0,87. While in the obese group the mean value of delta FEV1 is -0.17, FVC of 0.00, and lung function of -4.92. The conclusion of this study is the change of lung function between normal and obese group showed a significant difference, that is an increase in the normal group, but in the obese group actually decreased. Keywords: Obesity, Lung Function, Exercise Induced Bronchospasm (EIB), exercise PermaLink | Plain Format | Corresponding Author (Komang Trisna Sumadewi)

207	Biology	ABS-400
ANTIOXIDANT ACTIVITY FROM ETHANOL EXTRACT OF KUPA LEAVES (Syzygium polycephalum (Miq.) Merr & L. M. Perry) USING DPPH (2,2- diphenyl-1-picrylhydrazil) METHOD Farid Perdana1*, Isye Martiani1, Dhanti M A1 1. Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam Univesitas Garut Jl. Jati No. 42 B Tarogong Garut, Jawa Barat *farid[at]uniga.ac.id Abstract Kupa plant is one of the endemic plants of Indonesia which is included in the tribe of guava (Myrtaceae). This study aims to determine the antioxidant activity of ethanol extract of kupa leaves (Syzygium polycephalum (Miq.) Merr & L. M. Perry), and to determine the class of metabolites available in simplicia and ethanol extract of kupa leaves. Extraction of kupa leaf simplicia was carried out by maceration method using ethanol 96%. The antioxidant activity test was carried out qualitatively and quantitatively. The qualitative antioxidant test was carried out by Thin Layer Chromatography using the developer chloroform: methanol: acidic format (8:2:1) and using a sitroborate and DPPH 0.2% spotting appearance. Quantitative testing of antioxidant ethanol extract of Kupa leaves was carried out using the DPPH method with vitamin C as a comparison. The TLC test results obtained a greenish color on the sitroborat and yellow on the DPPH with an rf value of 0.381. In the antioxidant test the kupa leaf ethanol extract obtained an IC50 value of 10,327 ppm which is a very strong antioxidant. And secondary metabolites found in simplicia and ethanol extract of kupa leaves are flavonoids, saponins, steroids / triterpenoids, quinones and phenols. Keywords: Antioxidants, kupa leaves, ethanol extract, DPPH. PermaLink | Plain Format | Corresponding Author (Farid Perdana)

208	Biology	ABS-146
Potency of Cajuput Leaf Waste as Additional Feed Concentrate Ana Widiana (a*), Rizal M Hasby (a), Ukit (b) (a) Faculty of Science and Technology. UIN Sunan Gunung Djati Bandung. *anawidiana[at]uinsgd.ac.id Jl. AH. Nasution No. 105 Bandung West Java Indonesia (b) Faculty of Tarbiyah and Teaching Programme. UIN Sunan Gunung Djati Bandung Jl. AH. Nasution No. 105 Bandung West Java Indonesia Abstract Utilization of cajuput leaf waste as a sheep feed, especially protein content (10.65%) has not met the SNI standard (> 15%).The addition of concentrate with high protein content is expected to increase the protein content in feed and improving the quality of feed (especially ammonia (NH3) production in rumen).The purpose of this research was to know the effect of addition of concentrate in increasing the protein content and also the potential of cajuput leaves waste mixture with field grass as a sheep feedby in vitro test. The experiment used a complete randomized design with three treatments(R1 = 50% field grass + 50% concentrate; R2 = 25% cajuput leaves waste + 25% field grass + 50% concentrate; R3 = 50% cajuput leaves waste + 50% concentrate)with four repetitions. Statisticstest with Anova followed by Duncan test. The results showed that the addition of concentarte to mixtured of waste cajuput leaves and field grass can increased the protein content in feed and affectedtothe production of NH3, VFA, and the digestibility of feed.From various aspects,treatment of R2 most likely to be used for sheep feed. Keywords: feed, in vitro, Melaleuca cajuputi, Waste PermaLink | Plain Format | Corresponding Author (Ana Widiana)

209	Biology	ABS-147
Antibacterial Activities Test of Cajuput Leaf Waste Extract (Melaleuca cajuputi Powell) on Pathogenic Bacteria Ukit (a), Ana Widiana (b*), Evi Rahmawati (b), Rizal M Hasby (b) (a) Faculty of Tarbiyah and Teaching Programme. UIN Sunan Gunung Djati Bandung. Jl. AH. Nasution No. 105. Bandung. West Java. Indonesia (b) Faculty of Science and Technology. UIN Sunan Gunung Djati Bandung. Jl. AH. Nasution No. 105. Bandung. West Java. Indonesia *anawidiana[at]uinsgd.ac.id Abstract Cajuput plants (Melaleuca cajuputi Powell) and its waste are one of the plants that can produce essential oils. Cajuput plant essential oil has the largest compound component, 1.8 cineol which has the ability as an antibacterial to kill pathogenic bacteria. The purpose of this study was to determine the antibacterial activity of cajuput leaf waste extract against pathogenic bacteria and find out the category of inhibition zones formed from extracts of cajuput leaf waste. This study used a completely randomized design method. Cajuput leaf waste used has a shelf life of 0 months, 2 months and 4 months old. The pathogenic bacteria used consisted of 17 isolates, each treatment was repeated twice. The results showed that cajuput leaf extract had antibacterial activity known by the formation of inhibitory zones. The inhibitory zone in the extract of the 0-month leaf waste was greater than in the leaves extract of leaves of 2 months and 4 months. The diameter of the inhibition zone is at most 13 mm and at least 1 mm. With these results, it can be concluded that the antibacterial activity of eucalyptuscajuput leaf waste belongs to the category of weak to strong. Keywords: Cajuput leaf waste, antibacterial, pathogenic, inhibition zone PermaLink | Plain Format | Corresponding Author (Ana Widiana)

210	Biology	ABS-659
Characteristics of Maternal Maternity with Early Rupture of Amniotic Events in Mangusada General hospital Ni Putu Diah Witari (a*), A.A Istri Agung Pradnya Dewi Wirawan (a), Wayan Semadha (a), Anak Agung Ngurah Agung Putra Wirawan (b) a) Faculty of Medicine and Health Sciences, University of Warmadewa, Denpasar, Bali *) diahwitari[at]gmial.com b) Mangusada General hospital, Badung, Bali Abstract Premature rupture of the membranes is the spontaneous rupture of the membranes when it has not shown signs of labor, or if one hour later there are no early signs of labor. Some risk factors for premature rupture of membranes in the mother can be influenced by spelling factors, maternal age, parity, gestational age and a history of premature rupture of membranes (KPD). The purpose of this study was to determine the characteristics of maternity with premature rupture of membranes at Mangusada General hospital. This research is a descriptive study using secondary data. The results showed that the characteristics of maternity with early rupture of amniotic events in Mangusada General hospital were based on the highest maternal age at the age of 20-35 as many as 175 cases (70.6%), based on the highest age at term (> 34 weeks) by 213 cases (85.9%), based on the most parity occurred in nulliparous as many as 134 cases (54.0%), based on the majority of maternal occupations in private employment as many as 122 cases (49.2%), based on the previous history of KPD the most occurred in mothers without history Previous KPD was 218 cases (87.9%). Keywords: premature rupture of membranes, Mangusada General hospital PermaLink | Plain Format | Corresponding Author (ni putu diah witari)

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