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211	Biology	ABS-404
Production of Stringbean (Phaseolus vulgaris.L) using Phosphate Solubizing Bacteria and Natural Phosphat Fertilizer in Acid Soil Cecep Hidayat, M. Agfar Pratama, Adjat Sudrajat Agrotechnology Dept, UIN Sunan Gunung Djati Bandung Abstract Acid soil can be used for land extensification, but need input technology in order these soil to give maximum support for crop growth. The aim of the reserach was to know the effect of Phosphate Solubizing Bacteria and Natural Phosphat Fertilizer on the production of Stringbean ( Phaseolus vulgaris.L ) The aim of the research was AMF and organic matter on the growth and yield of Capsicum annum L. A field trial at Unpad research station 768 m abl had been carried out using randomized block design two factors with factorial pattern and three replications. The first factor is Phosphate Solubilizing Bacteria (0 ml, 10 ml, 20 ml, 30 ml, while the second factor is the type of natural phosphate fertilizer (Guano 120g polybag-1, rock phosphate 120g polybag-1 and crab flour 120g polybag-1). The results showed there was no interaction effect of Phosphate Solubizing Bacteria and Natural Phosphat Fertilizer on harvest index. The type of natural phosphate fertilizer has an independent effect on leaf area and pod dry weight. Keywords: Phosphate Solubizing Bacteria, Natural Phosphat Fertilizer, Acid Soil, Production PermaLink | Plain Format | Corresponding Author (cecep hidayat)

212	Biology	ABS-405
The Effect of Swimmer crab flour (Protunus pelagicus) and Arbuscular mycorrhizal fungi on flowering and yield of Japanese cucumber Cecep Hidayat, Yusan Ahyar, and Yati Setiati Agrotechnology Dept, UIN Sunan Gunung Djati Bandung Abstract Swimmer crab flour (Protunus pelagicus) which has high Ca content and Arbuscular mycorrhizal fungi (AMF) can be used to overcome acid soil. The aim of this research was to know the interaction effect of swimmer crab flour and AMF on increasing acidic dry soil productivity for the growth and yield of Japanese cucumber plant. The polybag research was conducted at Nagreg Subdistrict from May to July 2018 using Randomized Block Design with two factors and three repilactions. The first factor was swimmer crab flour doses (control, r1= 9 g 5 Kg soil-1, 12 g 5 kg soil-1,15 g 5 kg soil-1 and 18g 5 Kg soil-1) and the second factor was AMF dosage (control, 5 g crop-1 and 10 g crop-1). The results showed that there was an interaction effect of swimmer crab flour and AMF on the root infection degree , there was an independent effect of swimmer crab flour on flowering time, dry weight of fruits, and harvest index of cucumber, and there was an independent effect of AMF on the harvest index. Keywords: acid soil, swimmer crab flour, AMF, yield, japanese cucumber PermaLink | Plain Format | Corresponding Author (cecep hidayat)

213	Biology	ABS-662
The Differences in The Type of Packaging and Storage Time for The Quality of Tilapia Fish Boiled with Balinese Spices at Cold Temperature I Gede Suranaya Pandit (a,b,*), Pande Ayu Naya Kasih Permatananda (c), Rhini Wulandari (a) (a,*)Faculty of Agriculture, Universitas Warmadewa, Denpasar, Indonesia suranaya_pandit[at]yahoo.com (b)Post Graduate Program, Universitas Warmadewa, Denpasar, Indonesia (c) Faculty of Medicine and Health Science, Universitas Warmadewa, Denpasar, Indonesia Abstract Tilapia fish that is boiled with Balinese spices or it is also called by Balinese people as nila nyat nyat, exactly is one of the most popular Balinese dishes because it is very tasteful. But until now, none has done packaging and storing it at cold temperature for resale. The aim of this study is to understand the interaction among the differences in type of packaging and storage time on quality of tilapia fish that is boiled with Balinese spices. We used factorial completely random design with four replications, first factor is type of packaging, that is consisted of 2 levels: A1=Styrofoam box and A2=Plastic box, second factor is storage time, that is consisted of 3 levels: B1=0 day, B2=3 days, B3=6 days. Objective observation includes water content, protein content, total volatile bases, total plate count, whereas subjective observation or organoleptic such as appearance, texture, odour, and taste. The result of the study showed that there was no interaction between the type of packaging and storage duration on the quality of tilapia fish that is boiled by Balinese spices. Based on objective observation, storage time at cold temperature still maintain good quality of tilapia until sixth day. Keywords: Type of packaging, storage time, tilapia, nila nyat nyat PermaLink | Plain Format | Corresponding Author (I Gede Suranaya Pandit)

214	Biology	ABS-409
AN EXPLORATIVE STUDY ON THE USE OF CONDOM IN DIRECT COMMERCIAL SEX WORKERS IN SANUR Luh Gede Pradnyawati Faculty of Medicine & Health Sciences of Warmadewa University Abstract Previous sudies on commercial sex workers reported that the number of commercial sex workers spreading throughout Denpasar city and its surroundings is 250 persons and 14% of them had been infected with HIV/ AIDS (Human Immunodeficiency Virus/ Acquired Immune Deficiency Syndrome). However, referring to the data in the City Health Service of Denpasar, the number of direct commercial sex workers throughout Sanur area in 2017 is 450 persons. Such number confirms that the risk of sexually transmitted infection among the direct commercial sex workers in the area is intensely high. Condom use is seemingly ruled out. Thus, by the present study, an explorative investigation is carried out to explore more about the condom use among the direct commercial sex workers in Sanur. The study applies qualitative research design. A purposive sampling technique was implemented in selecting 15 informants. The 15 informants included the direct commercial sex workers, pimps, and customers. These informants were provided with in-depth interviews. The results of data analysis showed that during sexual intercourses not all the direct commercial sex workers applied the use of condoms. The leading reason was the male partners disliked the condoms in the sexual intercourse. However, it is also found that economic factor had given rise to avoiding the condom use. It should be noted well that using condoms during sexual intercourse decreases the growing number of the direct commercial sex workers. Keywords: Condom, Sexually Transmitted Infection, Commercial Sex Workers, Sanur PermaLink | Plain Format | Corresponding Author (Luh Gede Pradnyawati)

215	Biology	ABS-925
Telomerase Content (TERT) in Limphocytesof White Rats (Rattus norvegicus)in Different Ages Rini Puspitaningrum1*, Mohamad Sadikin2, Adiati Bintari Ayu1 1Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Indonesia 10520 2 Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Indonesia Abstract Aging getting more attention in meetings both in between scientists as public. Long telomeres on lymphocytes able predict the aging process and related to change telomerase function. This research aimed to measure levels telomerase enzyme on lymphocytes cell with age 6-8 weeks and 2-3 years. This research used experimental method. This researchwas conductedon February-May 2018 in Laboratory Biochemistry and Biology Molecular Faculty Medical University of Indonesia. Based on the research that has been done, the level of telomerase enzyme in Rattusnorvegicus lymphocytes within age 6-8 weeks had an average 2.24 ± 0.09 pg / mL lymphocyte. Telomerase enzyme levels in lymphocytes aged 2-3 years had an average 2.27 ± 0.15 pg / mL.This research obtained accurate information that can be used to find out each individual who doesn’t show the same level at the same time, was not statistically significantly different. This is an interesting thing to do for a more heterogeneous environment and by paying attention to individuals per individual from various daily activities. Keywords: Aging , telomerase enzyme, lymphocytes, cell degeneration PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

216	Biology	ABS-158
Neuroprotective of Dunaliella salina on Fruit Fly Model of Parkinsons Disease Exposed to Paraquat Mohamad Agus Salim(1), Muhammad Subandi (2), Yeni Yuniarti(3) (1) Biology Department, Science and Technology Faculty of UIN Sunan Gunung Djati Bandung (2) Agrotechnology Department, Science and Technology Faculty of UIN Sunan Gunung Djati Bandung (3) Mathematic Education, Universitas Pendidikan Indonesia Abstract Microalgae of D. salina possesses multiple pharmacological properties which are mainly attributed to the active compounds such as polyphenol, chlorophyll and B-carotene. However, the utilization of D. salina as a neuroprotective agent in Parkinsons disease is still questionable. In present study, we observed the potential neuromodulatory effects of D. salina extract against the toxicity exposed to paraquat in fruit fly Drosophila melanogaster. Male wild-type fruit flies were concomitantly induced by paraquat (3.5mM) and D. salina methanolic extract (200 ug/mL) in their diet during 4 days of observation. Paraquat fed fruit flies had exhibited a higher incidence mortality and the worst influence on locomotor phenotypes (negative geotaxis) when compared to the control. D. salina extract treatment showed protection ability against these deleterious effects of paraquat. Though, paraquat exposure caused a marked increase in malondialdehide (MDA) content as indicating increase lipid peroxidation process in brains of fruit flies. Meanwhile, paraquat toxicity was also related to a significant decrease on dopamine level in head of fruit flies, which were attenuated by D. salina extract treatment. Our results conclude that D. salina extract significantly reduced the toxicity of paraquat compounds exposed to fruit flies and proved the usefulness of this model as a potential therapeutic strategy for symptoms that appear in Parkinsons disease. Keywords: Dunaliella salina ; Fruit fly ; Paraquat ; Parkinson PermaLink | Plain Format | Corresponding Author (Mohamad Agus Salim)

217	Biology	ABS-670
The Correlation between Sleep Duration with Concentration Level on Elementary School Students in Denpasar AAA L Paramasatiari and LAP lestari Faculty of Medicine, Warmadewa University Abstract Introduction: Concentration disorder is a condition thats difficult to focus attention. The factors that influence concentration are environment, sociality and psychology. Concentration can also be influenced by a person sleep duration. This can affect academic achievement at school. Objective : This research aims to find out the correlation between sleep duration, and concentration level in Elementary School students in Denpasar Methode: The research design was cross-sectional study; data were collected using questionnare at three elementary school in Denpasar. The amount of the subjects were 96 respondents was selected by multistage cluster random sampling method from Harapan Mulia, SDN19 Pemecutan, SDN 27 Pemecutan elementary school. Data was analyzed using Chi-Square statistical test by Statistical Package for the Social Sciences for Windows 22 (SPSS) program. Result : The results showed 96 respondents collected from elementary school; 49% were males and 51% were females. The most aged of student were 10 years old. The low, medium and high concentration level were 27,1%; 46,9%;26% respectively. The less and enough sleep duration were 67,7%; 32,3% respectively. The percentage of non-breakfast subject was 35 persons (36,5%) and the breakfast subject was 61 persons (63,5%). The result was showed that there was a significant relationship (p<0,05) between sleep duration, with the level of concentration. Conclusion: The conclusion can be drawn that there was a positive and significant relationship between sleep duration and concentration level on Elementary School students in Denpasar. Keywords: Sleep duration, Concentration Level, and Elementary School Students PermaLink | Plain Format | Corresponding Author (Anak Agung Ayu Lila Paramasatiari)

218	Biology	ABS-426
NUTRITIONAL STATUS OF CHILDREN AGE 4-6 YEARS OLD IN LOCAL VILLAGE, BALI Pande Ayu Naya Kasih Permatananda(a*), Ni Wayan Ari Sawitri (b), Nyoman Gina Henny Kristianti(b) (a) Faculty of Medicine and Health Science, Universitas Warmadewa, Denpasar, Indonesia *nayakasih[at]gmail.com (b) Faculty of Medicine and Health Science, Universitas Udayana, Denpasar, Indonesia Abstract Malnutrition is the primary cause of morbidity and mortality in children. The term of malnutrition includes a state of undernutrition and overnutrition. The purpose of this study is to assess the nutritional status of children age in local village, Bali. This research was a cross-sectional descriptive study with 60 subject which were kindergarten students in Bali and with 60 respondents which were the mother of the subjects. The data collecting technique of this research was purposive sampling, with using questionnaire for respondents and direct measurement for the subjects. Most of the respondents were in normal category based on anthropometry index. We also found the prevalence of overnutrition were higher than undernutrition. Undernutrition status was found more in children with less nutrient intake, normal nutrition status was found more in children with good nutrition intake, while overnutrition status was more dominant in children with high nutrition intake. Measurement of nutritional status for children is very important as anticipation data for further research and basis for making public health programs. Keywords: nutritional status; children age 4-6 years old; nutritional intake PermaLink | Plain Format | Corresponding Author (Pande Ayu Naya Kasih Permatananda)

219	Biology	ABS-938
Serum Calcium Concentrations in Chronic Hemodialysis Patients in Sanjiwani Gianyar Hospital, Bali Sri Wardani N.W, Budiyasa D.G.A, Sri Masyeni, D.A.P Faculty of Medicine and Health Sciences, University of Warmadewa, Bali, Indonesia Abstract Abstract. Chronic Kidney disease-mineral bone disorder (CKD-MBD) are complication of patients on chronic hemodialysis therapy. This study is a cross-sectional descriptive analytic study aims to examine concentration of serum calcium as a marker of CKD-MBD. Calcium serum concentration were examined with spectrophotometer method than we computed into low, normal, and high serum calcium concentrations. This study recruited 100 chronic hemodialysis patients at Sanjiwani Gianyar Hospital and AriCanti Hospital, composed of 67 male and 33 female, mean of age is 52.52 ±12.81, duration of HD were 44,6 month. Most of the patients undergone hemodialysis 2 times a week (96 %) and performed HD 4.5 hour per session and 66% patients used AV fistula access. This study was found most of chronic hemodialysis patients (60%) has low serum calcium concentration <8.5 mg/dl. On Spearman correlation test, serum calcium concentrations in this study were significant influenced by serum creatinine concentration r = -0.461 and phosphate serum concentration r= -0.0273 on p < 0.001, respectively. Conclusions: most of the chronic hemodialysis patients in this study has low serum calcium concentration as a marker of CKD-MBD. Keywords: CKD-MBD, hemodialysis, serum calcium concentration PermaLink | Plain Format | Corresponding Author (Sri Wardani)

220	Biology	ABS-428
THE EFFECT OF BINTARO FRUIT (CERBERRA MANGHAS) EXTRACT ON COCKROACH MORTALITY (PERIPLANETA AMERICANA) C Ardiana, U Sayidah, L Amalia, I D Saputra, R Muthmainnah Biology Department, Institut Pendidikan Indonesia Abstract Abstract Bintaro fruit contains a lot of organic compounds that cause mortality of a number of insects including cockroaches. The reasearch aims to determine the effect of various concentrations of bintaro fruit (Cerberra manghas) as bioinsecticides, and to know the effective concentration to achieve mortality from Periplaneta americana cockroaches . The method used in this study is a pure experimental method that uses Completely Randomized Design (CRD) with 5 treatments and 5 repetitions, with different concentrations namely concentrations of 0%, 10%, 20%, 30%, and 40%, so that the sum of all a sample of 125 Periplaneta americana cockroaches. Based on the results of statistical tests using the ANOVA test and Scheffe further test, the treatment of the concentration of bintaro fruit extract (Cerberra manghas) 40% significantly affected the Periplaneta americana cockroach compared with other treatments and in terms of LC50 values, is the mortality rate had reached 50% in observations 5 x 4 hours and more mortality than other concentrations. Keywords: Bintaro Fruit Extract (Cerbera manghas), Cockroach Mortality (Periplaneta americana) PermaLink | Plain Format | Corresponding Author (Chevi Ardiana)

221	Biology	ABS-685
How will Personality Combined with Motivation Affect Student Learning Outcome of Ecosystem Course Supriyatin, Diana Vivanti Sigit, Friska Matdalena Departement of Biology Education, Universitas Negeri Jakarta Jl. Rawamangun Muka, Jakarta, Indonesia Abstract Learning outcome someone is determined by two factors, namely internal factors and external factors. Personality and motivation including internal factors that affect student learning outcome. This research aims to determine the correlation between personality and motivation with student learning outcome. Participants in this research are students of XI grade majoring in science from 13 Senior High School Jakarta. This research was held on August until September 2018 with 121 students of XI grade majoring in science chosen by simple random sampling. This research used survey technique through correlative study. A correlation analysis revealed that personality and motivation were significant relationship with student learning outcome. Personality and motivation simultaneously provides a considerable contribution with student learning outcomes. Keywords: motivation, personality, student learning outcome. PermaLink | Plain Format | Corresponding Author (Supriyatin Supriyatin)

222	Biology	ABS-687
Relationship Between Screening Results of Development using KPSP Method and Results of Measuring Growth Parameters in Children in Denpasar Dewa Ayu Agung Alit Suka Astini, Komang Trisna Sumadewi Department of Anatomy, Medical Faculty and Health Science of Warmadewa University, Bali-Indonesia Abstract Measurement of growth and development in children is the most important thing related to early and appropriate management that can be done if deviations are found. Growth measurements are measurements of the childs weight, height and head circumference. Some previous studies have shown that there are many delays in child development. There are several ways that can be used to screen development, one of them is by using a developmental pre-screening questionnaire that requires special officers trained in its implementation. The results of measurements of growth are often associated with development in children. Based on this, further research is needed regarding the relationship between screening for development and growth in children. The purpose of this study was to analyze the relationship between the results of screening on development using the KPSP method and the results of measuring growth parameters in children aged 0-6 years in Denpasar. The study was conducted using cross sectional analytic. Based on the Pearson correlation test (α = 0.05), it shows that there is a significant negative correlation (r = -0.218; p <0.05) between weight and development. This shows that the higher body weight will further reduce the level of child development. Keywords: growth, development, developmental questionnaire, weight gain PermaLink | Plain Format | Corresponding Author (Dewa Ayu Agung Alit Suka Astini)

223	Biology	ABS-176
Screening of the drought tolerant phosphate solubilizing bacteria in dissolving P-inorganic Lolita Endang Susilowati, Bambang Hari Kusumo, Zaenal Arifin University of Mataram Abstract The drought tolerant phosphate solubilizing bacteria is needed to dissolve phosphate (P) inorganic which is usually low in availability at dry land. This study is aimed to obtain a consortium of drought tolerant phosphate solubilizing bacteria which has high potential in dissolving P-inorganic. An experimental study which has 4 treatments of bacterial combinations has been conducted in a laboratory. The first treatment was the combinations of Pseudomonas azotoformans (PA), Acinetobacter baumannii (AB) and Bacillus paramycoides (BP). The second treatment was the combination of PA and AB. The third treatment was PA and BP, then the fourth treatment was AB and BP. The potential of the consortium bacteria in dissolving P-inorganic was measured qualitatively using phosphate dissolution index (FDI) on solid pikovskayas medium. While, the potential of the consortium bacteria in dissolving P-inorganic was measured quantitatively by measuring dissolved P using the ascorbic acid method in liquid pikovskayas medium containing 0.5% Ca3(PO4)2. The results showed that the consortium of those three bacteria (PA AB and BP) has the best potential to dissolve P-inorganic compared to other combinations. In addition, the three bacteria combination also resulted in the highest dissolved P with 0.29% potential dissolution, followed by combination of AB and BP (0.19%), and PA and BP (0.18%), respectively. Thus, before the consortium of these three bacteria is applied in soil, it is needed further experiment of potential of the bacteria in dissolving soil P-inorganic. Keywords: drought tolerant, phosphate solubilizing bacteria, dissolved P-inorganic, pikovskayas medium PermaLink | Plain Format | Corresponding Author (Lolita Endang Susilowati)

224	Biology	ABS-688
Students Ecoliteracy based on Structrure of Observed Learning Outcomes (SOLO) Taxonomy Ade Suryanda, Erna Heryanti, Hilda Nur Indah Lestari Department of Biology Education, Universitas Negeri Jakarta Jl. Rawamangun Muka, Jakarta, Indonesia Abstract This research inteant was to determine differences in the ecolitercy of natural sciences and social sciences. Ecoliteracy of students is needed to form a sustainable society, and a basic understanding of the ecology and principles of ecosystems is needed. Structure of Observed Learning Outcomes (SOLO) is used to find out the responses of students in their understanding of ecoliteracy. The research sample came from students majoring in natural sciences and social sciences academic year 2018/2019 in 3 Senior High School in Bekasi. The results showed that there were differences in the ecoliteracy of high school students majoring in science and social science that average ecoliteracy score of science students is 179 while the average ecoliteracy score of social studies students is 169. The level of SOLO taxonomy level of science students is at the relational and extended abstract level and the SOLO taxonomy level of the social students is at the multistructural level up to the extended abstract level. The conclusion of this study is that there are differences in the ecoliteracy of students from science and social studies subject to taxonomy Structure of Observed LearningOutcomes (SOLO). Keywords: Ecoliteracy, Taxonomy SOLO PermaLink | Plain Format | Corresponding Author (Ade Suryanda)

225	Biology	ABS-944
Blood gas Analysis of Bajau Sea Nomads Tribe Divers Indonesia Rini Puspitaningrum1, Azzahra Humairrah2, Yanti Susianti3, Erfan Handoko4, Arina Khairunnisa3, and Azzahra Mutmainah5 1. Department of Biology, FMIPA Universitas Negeri Jakarta 2. Human Medicine, JLU Giessen Germany 3. Medical Doctor, UIN Syarif Hidayatullah Jakarta 4. Department of Physics, Universitas Negeri Jakarta 5. Department of Biological Educational, FMIPA Universitas Negeri Jakarta Abstract The Bajau sea nomads as a famous tribe Indonesia. Bajau can breathe in sea water more than 3 minutes. There have been studies conducted on the Bajau tribe before, but this research will discuss the analysis of the Bajau tribes blood gas. The 20 people from the Bajau Tribe agreed, after the interview was taken, blood would be collected to find out how much PaO2 was. It was found that all the Bajau tribes had a PaO2 yield of more than 95%, which indicated that the oxygen in their blood was very high. Average depth above 3 meters. Keywords: Bajau sea nomads tribe, diving, breathing adaptation PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

226	Biology	ABS-434
The Potency of Seagrass community to Support the Development of Ecotourism in South Coastal of Lombok Island Indonesia Abdul Syukur, Lalu Zulkifli and Agil Al Idrus 1)Postgraduate Program of Science Education, Mataram University 2)Department of Sciences Education, Faculty of Teacher Training and Education, Mataram University 12*Correspondent Author: Abdul Syukur, syukurbiologi[at].unram.ac.id. Abstract Coastal ecosystems consisting of mangrove ecosystems, seagrass beds and coral reefs have the potential to be developed as ecotourism. Seagrass ecosystems located in the intertidal zone are an integral part of coastal tourism in the area of study. However, its existence has not received serious attention as a component of coastal ecotourism that can contribute to ecological services. This article aims to start an effort towards empowering this potential through the study seagrasses ecology to formulate the potential of ecological services in supporting the development of ecotourism. Data collection were done through observation, survey and questionnaire methods, and data analysis were done descriptively. The analysis showed that the potential of seagrass community tourism services can be grouped into three parts, namely: (1) seagrass community that form seagrass beds in each tourist location, (2) the diversity of species of biota associated with seagrass can be enjoyed by tourists and (3) seagrass community area can be the location of snorkeling at the time of high tide. The conclusion of the study is that the existence of seagrass ecosystems can provide added value from aspects of environmental services in the development of ecotourism in the southern coastal region of Lombok Island. Keywords: Seagrass ecosystem, Development of coastal ecotourism, Ecologycal services PermaLink | Plain Format | Corresponding Author (Abdul Syukur)

227	Biology	ABS-949
Antiretroviral therapy substitution among HIV/AIDS patients visiting Sanjiwani Hospital, Bali Sri Masyeni, IDG Wedha Asmara Sanjiwani Hospital/Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Abstract Background: Substitution of long-term ART exposed to HIV/AIDS patients are common due to the ART adverse event. The study aim is to describe the reason of ART substitution among HIV/AIDS patient at Sanjiwani Hospital Bali. Methods: A retrospective study has conducted to the medical records of HIV/AIDS patients at Sanjiwani hospital Bali, during 2006-1018. Clinical data retrieved from the medical records and presented as descriptive data. Results: Over a twelve-year period, 1112 HIV/AIDS patients evaluated in the study. The ART regimen were Zidovudine-based ART, Tenofovir-based ART and Stavudine-based, for 12.2%, 87.3%, 0.5%, respectively. There were 2.2 % switching of ART during the study periode. The most reason was anemia (48%), followed by reducing of kidney function (28%), allergic reaction (16%), and 4% of nausea and suspected clinically failure to ART. Conclusion: We highlight that anemia is the main reason of ART substitution among HIV/AIDS patients Keywords: ART; substitution; HIV/AIDS PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

228	Biology	ABS-950
SERUM PHOSPHAT LEVEL AMONG CHRONIC KIDNEY DISEASE PATIENT ON CHRONIC DIALYSIS Sadguna, Sri Wardani, DGA Budiyasa, Sri Masyeni Sanjiwani Hospital/Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Background: the increase level of serum phosphate is common due to secretion failure of the kidney on chronic kidney disease patients (CKD). The study objective is to determine the level of serum phosphorus among the CKD patient on chronic dialysis in Gianyar Bali. Methods: a prospective cross-sectional study was conducted in 100 serum of the patient visiting dialysis clinic in Sanjiwani and Arisanti Hospital on October 2018. A three mL of blood was collected and the phosphate level was measured with specthrofotometer. Results: Sixty seven of 100 samples was man with mean age was 52,52 (SD±12,811). The most cause of CKD was pyelonephritis chronic. The mean of hemodialysis duration was 44,59 (SD± 32,40). Level of the phosphate more than normal limit found as high as 69% of the samples. There was a correlation between age (p=0.001), gender (p=0.020) and phosphate level, but there was no correlation between IMT, HD duration and phosphate level, accounting for p=0.222 and p=0.264, respectively. Conclusion High finding of hyperphosphatemia that found in the study reveal the presentation of CKD-mineral and bone disorder in CKD patients. Correlation of age with higher phosphate level may relate with the deterioration of kidney function in elderly. Keywords: CKD; phosphate level, hemodialysis PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

229	Biology	ABS-952
Performance of 0-14 Weeks-aged Super Free-range Hens that are Fed by Fermented Coconut Pulp Flour-Contained Ration Ni Ketut Etty Suwitari , Luh Suariani, Ni Made Yudiastari, I Nyoman Kaca, Yan Tonga Program studi Peternakan, Fakultas Pertanian Universitas Warmadewa Abstract The research was carried out at the Basic Laboratory of the Faculty of Agriculture, Warmadewa University with a level of use of fermented coconut pulp, namely R0 (ration that contains no fermented coconut pulp, R1 (ration containing 5% fermented coconut pulp flour), R2 (ration containing 10 % fermented coconut pulp) and R3 (rations containing 15% fermented coconut pulp). Each combination of treatments was repeated three (3) times, and each replication consisted of 4 super female free-range chickens. Variables observed included: 1). Age of chicken growth includes the weight gain and final weight of super free-range chicken, 2) Percentage of carcass and non-carcasses for super free-range chicken, 3) Feed Convertion Ratio (FCR, 4) Feed consumption, and 5) B / C ratio. From this study it was found that the giving of coconut pulp flour was fermented up to 10% (R2) significantly (P <0.05) could increase weight gain and final weight and B / C ratio of 14 weeks super female free-range chicken, and flour dregs fermented coconut to the level of 10% also decreases ration consumption and the conversion of ration for super female chicken 14 weeks old. Keywords: Performance, Carcass, Super Free-range chicken, Coconut Dregs, Fermentation PermaLink | Plain Format | Corresponding Author (Ni Ketut Etty Suwitari)

230	Biology	ABS-953
Relationship between the Waist Circumference on Obesity among the incidence patient of Type 2 Diabetes Mellitus in BRSU Tabanan Bagus Gede Ariesta Darma Hendra, Suyasning Hastiko Indonesiani Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Diabetes mellitus is one of the health problems worldwide. The prevalence of DM in Indonesia in 2013 is 12,191,564 people and is predicted to increase to 21.3 million people in 2030. Obesity is the main factor of type 2 diabetes mellitus. The measurement to diagnose obesity is the waist circumference. The aim of this study was to determine the relationship between the waist circumference on obesity among the incidence patient of type 2 diabetes mellitus in BRSU Tabanan. The design of this research was cross sectional. The subjects of this study were all adult male patients with type 2 diabetes mellitus or obesity in ambulatory clinic BRSU Tabanan, who were selected using consecutive sampling. Independent variables in this study was the waist circumference and the dependent variable was type 2 diabetes mellitus. Data were collected using instruments such as metric / meter cloth and blood glucose was measured using Easy Touch GCU. Data were analyzed by SPSS application using Chi-square. The results were as follows: the average of the waist circumference (obesity) and type 2 diabetes mellitus were 52,8% and 54,7% respectively. The relationship of both above was significant (p<0,05; r=0,396). The conclusion could be drawn that there was positive significant relationship between obesity and type 2 diabetes mellitus. It was recommended that the subject should be maintain the ideal body weight, and for the obese people were expected to reduce their body weight. Keywords: type 2 diabetes mellitus, obesity, waist circumference PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

231	Biology	ABS-955
Prevalensi of asthenopia among visual display terminal operator (VDT) in federal international finance group, Bali Sagung Putri Permana Lestari Murdhana Putere, I Putu Oka Primantara Faculty of Medicine and Health Sciences, University of Warmadewa Abstract The desire to create the best possible work, increased demands such as accuracy and speed of work cause employees to stay in front of the computer for long periods of work, so that it may be at risk of asthenopia disturbance. One of the occupations that are at risk for impaired asthenopia are employees who use computers at the Federal International Finance (FIF) Group Bali. This study aims to determine the percentage of asthenopia disorders based on asthenopia symptoms experienced. This research uses descriptive method with cross-sectional approach. The variables included asthenopia disorders, age, gender, VDT settings, screen filters, duration of work, and rest duration, room and angle lighting formed between the top of the monitor and the midpoint of the screen. The sample of this research is all employees who use computer in FIF Group Bali. Data collection in this study using Computer Vision Quistionnaire using Likert scale that has been validated. Data were analyzed descriptively with univariate computer aid. The result of the research showed that the most common complaint was headache as many as 38 respondents (79.16%). The incidence of asthenopia in the age group of 21-40 years was a headache of 28 respondents (58.4%), and at the age of 41-60 years was a headache of 10 respondents (20.9%). Based on sex, the prevalence of asthenopia in males is headache as much as 21 respondents (43.8%), in women is close blurry vision as much as 17 respondents (35.5%). The highest prevalence of asthenopia in room lighting settings <200 lux was headache as much as 19 respondents (39.6%), while in room lighting 200 lux-500 lux was blurred vision with as many as 10 respondents (23%). Keywords: asthenopia, VDT operator, computer vision questionnaire, FIF Group Bali PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

232	Biology	ABS-701
CORRELATION OF PURIN INTAKE WITH URIC ACID LEVEL IN POSTMENOPAUSAL WOMEN IN DENPASAR, BALI Putu Sutisna, I Made Maha Prasetya, FACULTY OF MEDICINE AND HEALTH SCIENCES, WARMADEWA UNIVERSITY Abstract Hyperuricemia is a condition of elevated blood uric acid level above normal value. One of the risk factors that causes hyperuricemia is high purine intake. This study aimed to assess the relation between purine intake and blood uric acid level in postmenopausal women in the working area of Puskesmas II East Denpasar. Analytic observational method with cross-sectional approach used 100 postmenopausal women collected by simple random sampling technique. Data of daily purine intake from respondents were obtained by semi-quantitative food frequency questionnaires. The study results showed that daily purine intake of respondents was in the range of 142.48-708.47 mg/day and the average was 408.72 ± 153.50 mg/day. Levels of blood uric acid in respondents were in the range of 2.6-8.4 mg/dL and the average was 5.52 ± 1.661 mg/dL. Purine intake had the strongest effect on blood uric acid level (p = 0,019; OR = 3,198; 95% CI = 1,208-8,461) compared with hypertension and BMI (p = 0,040; OR = 2,573 ; 95% CI = 1.043-6.347; p = 0.378; OR = 1.521; 95% CI = 0.598-3.866, respectively). It can be concluded consume high purin diet cause elevated uric acid level among post menopausal women. Keywords: Purine, Uric acid, Postmenopause PermaLink | Plain Format | Corresponding Author (Putu Indah Budi Apsari)

233	Biology	ABS-957
Prevalence of obstructive sleep apnea in employee and lecturer of Faculty of Medicine and Health Sciences, University of Warmadewa Saktivi Harkitasari, Bintang Wibawa Putra Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Obstructive sleep apnea (OSA) is a respiratory illness with the involvement of sleep disorders that have a characteristic ofrecurrent collapse of pharynx airway during sleep. OSA has a serious physiological and psychological impact if left untreated. OSA complications can be divided into 2, namely neurocognitive dysfunction and cardiovascular disease. This will affect the quality of life of the patients, including low quality of sleep, reduced cognitive function, and decreased productivity. The purpose of this study is to determine the prevalence of high risk of OSA in employees and lecturers at Warmadewa University. This research is using descriptive research method with cross sectional design. The sample in this research is employee and lecturer of Faculty of Medicine and Health Sciences at University of Warmadewa taken at random. The independent variables were snoring, daytime sleepiness, obesity, and high blood pressure. Dependent variable is obstructive sleep apnea. The study used primary data, which the data collected by interviewing and distributing Berlin questionnaire and measuring body mass index of employees and lecturers of Warmadewa University. Data analysis used descriptive analysis with Statistical Package for The Social Science (SPSS) program. There were 12 (15.18%) employees and lecturers at high risk of OSA based on the Berlin questionnaire. It is expected that the puskesmas can optimize its role in carrying out advanced screening to detect OSA in the community. Keywords: Obstructive sleep apnea, prevalence of obstructive sleep apnea, employee and lecturer PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

234	Biology	ABS-702
Risk Factors of Miopia In Junior High School Students in Denpasar I Nengah Kapti,I Made Dharma Putra faculty of Medicina and health sciences, Warmadewa university Abstract Patient with refractive disorders in Indonesia almost reached 25% of the population or about 55 million people. Myopia is one of the refractive disorders in the eye that has a high prevalence in the world. The purpose of this study was to know the risk factors of myopia in junior high school students in Denpasar. This research was an analytical research with case control design with subjectstaken from junior high school students in Denpasar. School samples were choosed using cluster random sampling and the students by simple random sampling. There are 49 people who has miopia as the case and 49 people without it as the control. Data were collected by direct interviewed using prepared questionnaires, is analyzed using chi square test. The result showed significant correlation between genetic factor and myopia (p = 0,013; α <0,05), lifestyles (p = 0,02, α <0,05), and vitamins A, C, D and E (p = 0,039; α <0,05). It is expected that community healht care and schools to conduct routine checks, early detection of eye health and educate the lifestyle and vitamin consumption. Keywords: myopia, genetic, lifestyle, vitamins A, C, D and E. PermaLink | Plain Format | Corresponding Author (Putu Indah Budi Apsari)

235	Biology	ABS-958
Differences in the effectiveness of Metformin and Gliclazide to Lower Blood Glucose in Type 2 Diabetes Mellitus Outpatient in The Sanjiwani Hospital Sagung Putri Permana Lestari Murdhana Putere, Pande Putu Bagus Mahendra Yasa Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Nowadays, type 2 diabetes mellitus (T2DM) is the most prevalence of diabetes mellitus disease. In Indonesia 10 millions people suffering T2DM which caused Indonesia ranked seventh in the world and predicted to increase 16.2 millions patients on 2040. The use of oral antidiabetic drugs (OAD) like metformin and gliclazide still an option on T2DM therapy. This study aims to determine the differences in the effectiveness of metformin and gliclazide to lower blood glucose in patient T2DM in the sanjiwani gianyar hospital. The variable of this study is oral antidiabetic drugs and blood glucose. The design of this study using cross sectional analitic observational approach with retrospective study. The sample of this study is patient T2DM in internal medicine policlinic of Sanjiwani Gianyar Hospital from Januari-Desember 2016 with 160 subjects who selected through consecutive sampling and fulfil the inclusion and exclusion criteria. This study using secondary data in form of medical record. The data was analyzed by chi square test (α=0.05). The results was showed that the effectiveness of each OAD to decrease blood glucose for 3 months therapy, the used of metformin showed that (70%) 56 sample achieved controlled blood glucose while the used of gliclazide showed that (52.5%) 42 sample achieved controlled blood glucose and there was a significant differences in the effectiveness of metformin and gliclazide with p value is 0.035. The conclusions of this study is metformin more effective than gliclazide to lower blood glucose in T2DM outpatient and advices who gived to hospital, health workers and community. Keywords: Type 2 diabetes mellitus, Effectiveness, Metformin, Gliclazide, Blood glucose PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

236	Biology	ABS-960
Improving the quality of off-season fruit of Citrus cv. Siam through application of fertilization and pruning Ni Komang Alit Astiari (a*), Ni Putu Anom Sulistiawati (a) , Ida Bagus Komang Mahardika (a), and I Nyoman Rai (b) a Agrotechnology Study Program, Faculty of Agriculture,Warmadewa University, Denpasar, Bali. b Agroecotechnology Study Program, Faculty of Agriculture,Udayana University, Denpasar, Bali. * E-mail: alit.astiari[at]gmail.com Abstract This study aims to improve the quality of citrus cv. Siam through fertilization and pruning. This experiment used factorial randomized block design consisting of 2 factors. The first factor is fertilization consisting of 3 levels (P0 = only fertilized with manure/control, P1 = manure,N,P,K,Ca, and P2 = manure,N,P,K,Ca,Zn,Cu). The second treatment is pruning consisting of 3 levels (M0 =without pruning, M1 =pruning young shoots, and M2 =pruning young shoots, leaves and twigs that are protected and affected by disease). Fertilization treatment with P2 improved fruit quality, which was reflected by the increase of harvested fruit weight per tree (99.79%); weight per fruit (35.69%) and total dissolved solids (30.22%). Harvested fruit weight, weight per fruit, and total dissolved solids on treatment with P2 were 3442.76g, 173.58g, and 9.48obrix, respectively, but on control that were only 1673.13g; 127.92g and 7.28obrix. In the pruning treatment, the best results were obtained in M2 which was reflected in the increase in weight of fruit harvested per tree (40.91%), weight per fruit (19.56%) and total dissolved solids (25.55%). Fruit weight per tree, weight per fruit, and total dissolved solids in M2 treatment were 3638.77g, 173.58g, and 9.63obrix respectively, but in controls only 2582.34g, 145.92g, and 7.67obrix Keywords: fruit quality, off-season, fertilization, pruning, Citrus cv. Siam PermaLink | Plain Format | Corresponding Author (Ni Komang Alit Astiari)

237	Biology	ABS-961
The difference of musculoskeletal disorder between taxi d and ‘ojek’ driver in Kuta Ketut Satria Dananjaya Retayasa, Suyasning Hastiko Indonesiani Faculty of Medicine and Health Sciences, University of Warmadewa Abstract In developing countries the work-related Musculoskeletal Disorders (MSDs) is mostly occurs. The research of Indonesian Health Department in 2005 was found out most MSDs was due to worked-related disorders in their job. The aims of this study was to find out the differences of MSDs between the taxi drivers and taxi bike in Kuta District. The design of this study was cross sectional which was analyzed. There was 60 subjects were involved in this study which age ranged 25-50 years old and they had experiences more than one year. The MSDs was measured by Nordic Body Map quitionnaire with modified Likert scale. Data was analyzed by SPSS 23. The result were showed as follow: the average of MSDs of taxi drivers and taxi bike were 43,30±6,23 and 46,60±5,30, respectively. The difference of both above was significant difference (p<0,05). It was concluded that there was significant difference between MSDs of taxi drivers and taxi bike. Keywords: Taxi driver, taxi bike, musculoskeletal disorders, Nordic body map PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

238	Biology	ABS-450
Isolation, Characterization of Endophytic Bacteria from Mangrove Rhizophora mucronata Lam. and Antibacterial Activity Test Against Some Pathogenic Bacteria Baiq Intan Gina Maulani1) , Dewa Ayu Citra Rasmi1) , Lalu Zulkifli1*) 1)Deparment of Science Education, Faculty of Teacher Training and Education Mataram University *)Corresponding author : lalu_zulkifli[at]unram.ac.id Abstract Mangrove plants are widely used traditionally in treating various types of infectious diseases in the people of Lombok Island. This study aims to isolate and characterize endophytic bacteria from the roots, stems, leaves and fruits of mangrove Rhizophora mucronata Lam. which grows in Gili Sulat, and to determine their antibacterial activity against some patogenic bacteria, such as Escherichia coli, Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa. Media used for isolation were NA and TSA. Antibacterial activity tests were carried out with well diffusion methods, and the level of antibacterial activity refers to the category according to Vasanthakumari. Characterization of endophytic bacteria includes morphological of colonies, cell morphology, physiology. Eighteen (18) endophytic bacteria isolates were obtained. Results showed that 12 isolates of endophytic bacteria had moderate (8-12mm) to strong category (> 12mm) of their antibacterial activity against B. cereus and P. aeruginosa. Isolate of NBM2(1) gave the strongest antibacterial activity against B. cereus. It can be concluded that endophytic bacteria isolated from mangrove R. mucronata Lam. is highly potential to be developed as a new antibacterial source, especially against B. cereus pathogenic bacteria. Keywords: Endophytic bacteria, Antibacterial activity, R. mucronata Lam.. PermaLink | Plain Format | Corresponding Author (Lalu Zulkifli)

239	Biology	ABS-963
Relationship between Laundry Workers Workload and Fatigue Rate in Dalung Village, Kuta Utara District, Badung Regency I Gede Wahyu Balikusuma, Suyasning Hastiko I Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Laundry services develop as one of the leading informal economic sector in Bali. Workload on laundry worker is due to monotonous process. Workloads that are not in accordance with the ability of workers could lead to excessive energy use, resulting in fatigue. The aim of this study was to find out the correlation between workload and fatigue rate among the laundry workers in Dalung Village, Kuta Utara District, Badung Regency. The method used in this study is analytical descriptive with cross-sectional approach. The subject in this study were laundry workers in Dalung Village, Kuta Utara District, Badung Regency. The amount of the sample was 40 people selected by purposive sampling method. Workload data was measured by calculating the working heart rate using pulsemeter. Fatigue data was measured by 30-item fatigue questionnaire with Likert scale. The relationship between workload and fatigue rate were analyzed by SPSS using Somers d test. The results were shown as follows the average of the working heart rate and fatigue rate : 91.6 ± 8.27bpm and 57.03 ± 9.68, respectively. The highest percentage of workload category was light workload (82,5%) and the highest precentage of fatigue category was moderate category (80%). There was positive significant relationship between workload and laundry worker fatigue rate ((p <0.05 and r=0.47). The conclusion could be drawn that there was a positive significant correlation between workload and laundry worker fatigue rate. It was suggested that the workers should be drink water and rest enough to reduce fatigue. Keywords: workload, fatigue, laundry worker PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

240	Biology	ABS-453
BIOSURFACTANT- PRODUCING AND ANTHRACENE-DEGRADING BACTERIA FROM OIL CONTAMINATED SOIL Tri Handayani Kurniati (a*), Iman Rusmana (b), Ani Suryani (c), Nisa Rachmania Mubarik (d) a) Biology Study Program, Faculty of Mathematics and Natural Sciences, Jakarta State University. Jl, Rawamangun Muka, Rawamangun,. East Jakarta 13220. Indonesia. b) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Jl. Raya Darmaga, Bogor16680, West Java. Indonesia. c) Department of Industrial Technology, Faculty of Agriculture Technology, Bogor Agricultural University Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia. d) Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University. Jl. Raya Darmaga, Bogor 16680, West Java. Indonesia. Abstract Anthracene, together with other polycyclic aromatic hydrocarbons (PAHs), is a persistent and toxic soil contaminant because its low water solubility and high hydrophobicity. The effectiveness of PAH degradation could be enhanced by using biosurfactant. In this study, we isolated bacteria that were able to degrade anthracene and also produce biosurfactant. Samples were taken from oil contaminated soil at Jakarta Bay. Isolation was done in a Minimal Salt Medium (MSM) supplemented with 1% crude oil. Screening methods for biosurfactant production include Blood Hemolysis, Drop Collapse Assay and Oil Displacement Assay. Three isolates which have biosurfactant production and anthracene degradation capability was identified based on 16SrRNA gene. These isolates were identified as Ochrobactrum intermedium AMA9, Ochrobactrum tritici CHA60, and Pseudomonas stutzeri CRA7. Based on GC-MS analysis, Ochrobactrum intermedium AMA9 showed high ability to degrade 84,79% anthracene in liquid MSM medium supplemented by 100 ppm anthracene after seven days incubation. Keywords: anthracene; bacteria; biosurfactant; degradation PermaLink | Plain Format | Corresponding Author (Tri Handayani Kurniati)

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