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271	Biology	ABS-987
Level of Knowledge, Attitude, and Behaviour on Type II Diabetic Patient in the Diabetic Center Sanglah Central Hospital Denpasar 2017 About Their Illness’s Management Made Widya Wirayanti Puteri, IGP Wiadnyana Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Diabetes Mellitus (DM) is a chronic disease with high prevalence in Indonesia. Riskesdas in 2007 said that DM was the sixth leading caused of death in Indonesia with . Sanglah Central Hospital Denpasar is the only hospital in Bali that has Diabetic Center . Diabetic can be regarded as a disease that deplete property, therefore it need a real behaviour that is based on a good attitude and knowledge to avoid bad complications. This study aims were to determine the level of knowledge, attitude, and behaviour of type II DM patients in Sanglah Central Hospital Denpasar in 2017. The type of research used was descriptive with cross sectional approach. The number of respondents in this study were 70 patients, selected by consecutive sampling technique. This study used primary data which obtained through the direct respondent’s interviewed with prepared quesioner. Processing and data analysis were performed using SPSS. Characteristics of respondents were differentiated by sex, age, education, and lenght of treatment. The results showed 66 respondents (94,3%) have good level of knowledges, 53 respondents (75,7%) have good attitudes, and 43 respondents (61,45%) have good behaviour. The conclusion of this study is that most respondents have good level of knowledges, attitudes, and behaviours although many respondents found have less understanding about diet. Keywords: Diabetes mellitus, Management, Knowledge, Attitude, Behaviour PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

272	Biology	ABS-988
UTILIZATION OF NATURAL SURFACTANTS FROM SAPONIN COMPOUND OF FUEL WASTE (Cocos nucifera L.) AS A ENVIRONMENTAL FRIENDLY LIQUIDS DETERGENT I Kadek Irwan Setiawan, Anak Agug Ngurah Agung Pradnya Iswara Wirawan, Kartika Sari Faculty of Medicine and Health Sciences Warmadewa University Abstract The purpose of this study was to find out the results of the effectiveness of detergent detergency against stains on white cloth based on organoleptic tests and to determine the results of measurement tests for pollution levels with parameters of COD (Chemical Oxygen Demand), DO (Dissolve Oxygen) and pH levels in each each treatment. This research is an experimental study with a Completely Randomized Design (CRD) or Randomized Complete Block Design method, which takes 5 treatments, namely two types of control groups including negative control (K-) by adding distilled water and positive control (K +) by adding detergent contained on the market, and there are 3 treatment groups, namely P1, P2 and P3, respectively, Sanur Biodetergen with concentrations of 2.5%, 5% and 10% respectively. The instrument used for the extraction process is the Rotary Vacuum Evaporator and the instrument used for testing pollution levels is a set of titration tools with iodometric titration methods. The results obtained showed that the second treatment (P2) was the best treatment with an average detergency power of 3.10 or with faded parameters evenly with the edges of the stain that faded and had a minimum pollution level ie COD value 7.9 mg / L, DO value was 9.2 mg / L and pH level of 5.02, so that it qualifies as a low-contaminant liquid detergent that does not damage the environment. Keywords: Surfactant, Sanur Detergent, Saponin, Janur Waste (Cocos nucifera L.) PermaLink | Plain Format | Corresponding Author (Ni Luh Putu Eka Kartika Sari)

273	Biology	ABS-733
Balance of C (Carbon) and N (Nitrogen) of Bioslurry Fertilizer and Fertilization Dose on Leek Growth (Allium fistulosum) Pri Riznaya, Ana Rochana, Mansyur Universitas Padjadjaran Abstract The purpose of the research was to know the effect of C / N balance of bioslurry fertilizer (biogas sludge) and fertilizer dose on the growth of leek (Allium fistulosum). The design used was a complete randomized design of factorial pattern. The first factor of the C and N balances are 18, 20, 30 and 40. The second factor is the composting dose of 10 tons / hectare, 20 tons / hectare and 30 tons / hectare. Each treatment was repeated three times. The observed variables were the increase in length, width and number of strands. The data obtained were analyzed variance (ANOVA). The result of ststistic analysis shows that there is C / N influence on the number of strands; there was a dose effect on the length and width of leek but no interaction between C / N and the dose of fertilization. The best onion growth occurs at a dose of 10 tons / hectare as it leads to an increase in length, the highest width which is an important indicator of onion measurements. Keywords: C and N Balance, dose, leek, length increase, width and height. PermaLink | Plain Format | Corresponding Author (Pri Riznaya)

274	Biology	ABS-989
Nutritional Status Women of Reproductive Age Group with Vegetarian Diets in Badung Made Rusti Rama Yanti, IGP Wiadnjana Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Vegetarian diet has become a popular diet among people. However, the diet is known to cause the lack of some nutrients such as protein, iron, and B12, which has the implication on ones nutritional status. When the bodys metabolism is high as in times of growth, menstruation, pregnancy and lactation, and women of reproductive age group with vegetarian diets are vulnerable to nutritional deficiencies. The study is aimed to identifying nutritional status women of reproductive age group with vegetarian diets in Badung Regency. The method used in this study is descriptive quantitative, with cross sectional approach. Nutritional status based on Body Mass Index (MT), Upper Arm Circumference (LILA), and Hemoglobin (Hb) level. Data analysis in the form of frequency distribution table and narrated. The result of measuring the nutritional status obtained is most women of reproductive age group with vegetarian diets have good nutritional status. Can be seen based on IMT as much as 67.3%, based on LILA as much as 87.3%, and based on Hb as much as 38.2%. Nutrition problems found on the basis of IMT were 32.7%, among them less nutrition, more and obesity. Based on LILA obtained only 12.7% who have less nutrition, and based on Hb levels found most who experienced anemia as much as 61.8%. From these results it is necessary to note the intake of foods and types of food consumed for nutritional needs are met. Foods derived from iron and vitamin C that facilitate the absorption of iron. Keywords: Vegetarian, Nutritional Status, Body Mass Index (BMI), Hemoglobin Level (Hb), Ligkar Arm Up (LILA) PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

275	Biology	ABS-990
Phenophysiology of the formation and fall of flowers in overcoming efforts fruit-set failure in siam citrus plants Ni Putu Anom Sulistiawati (a*), Ida Bagus Komang Mahardika (a), Made Sri Yuliartini (a) a) Agrotechnology Study Program, Faculty of Agriculture, Warmadewa University, Denpasar, Bali. * E-mail: anomsulistia[at]gmail.com Abstract This study is entitled: Phenophysiology of the Formation and Fall of Flowers in an Effort to Overcome Fruit-Set Failure in Siam Citrus Plants. In this study the design used was a randomized block design, by observing how the stem position and the season affected the fall and forming flowers which finally affected the percentage of fruit-sets formed. Interaction between the location of flowers and the growing season showed a non-significant effect on the formation of flowers on the citrus plant. The average value between intervals and gadu shows no significant difference with each number of flower fall, namely: 152.57 (florets and 151.44 (florets). Percentage of fruit-set at the position of the upper, middle and lower stems shows differences which is not real, the most fruit-set formed is the top stem as much as 92.75% followed by the middle stem namely: 92.52%) and at least the percentage of fruit-set formed is the rootstock which is: 91.30%. Keywords: Deciduous fruit, deciduous flowers, endogenous, environmental, and fruit-set PermaLink | Plain Format | Corresponding Author (Ni Putu Anom Sulistiawati)

276	Biology	ABS-735
OPTIMUM SUSTAINABLE POPULATION ESTIMATION OF Rusa timorensis IN PANANJUNG PANGANDARAN NATURE RESERVE AND NATURAL PARK ASTRI YULIAWATI(a*), MILLA LISTIAWATI(a), YANTO SANTOSA(b), ACHMAD MACHMUD THOHARI(b) a)Biology Education Department, Universitas Islam Negeri Sunan Gunung Djati, Indonesia *astriyuliawati[at]uinsgd.ac.id b)Departement of Forest Resource Conservation and Ecotourism,Bogor Agricultural University. Indonesia Abstract Three main objectives of conservation are protection, preservation and sustainable utilization. Timor deer is one of Indonesian wild animal that has many benefits and high economic value. But the current timor deer population continues to decrease with population decline rate of 10% in each generation. It caused by harvesting without considering sustainability. Therefore it is necessary to manage the population for timor deer, so it can be available for sustainable use. Optimum sustainable population is a threshold population size for sustainable harvesting. The purpose of this study is to estimate the optimum sustainable population size so timor deer can be utilized sustainably in the future. The method used was projecting the actual population by using Leslie matrix (Density Dependence) so that population growth can be seen. The optimum sustainable population is the size of the population with the highest growth rate. The time used for this population projection is 100 years. Based on the results of this research, the optimum sustainable population size for timor deer in the pananjung Pangandaran nature reserve and natural park is 751 individuals and the optimum sustainable population size will be achieved in 2047. Keywords: Sustain Yield, Timor deer, Conservation PermaLink | Plain Format | Corresponding Author (Astri Yuliawati)

277	Biology	ABS-992
Correlation Between Knowledges and Attitudes Among Female Senior High School Students in Badung Toward Cervical Cancer and Their Participation in Human Papilloma Virus Vaccinations Putu Risma Yuvita Riadi, I Nengah Kapti Faculty of Medicine and Health Sciences, University of Warmadewa Abstract The prevalence of cervical cancer in Bali in 2015 was 0.7 ‰ with an estimated number of 1,438 people. The local government targeted "Bali Bebas Kanker Serviks 2020" by held primary prevention and early detection through IVA test, pap smear and HPV vaccinations. Government of Badung supports the program by subsidizing HPV vaccination to female high school students, but there were still many students who not participated because of many factors, i.e. knowledges and attitudes toward cervical cancer. The purposed of this studies were to determine the correlation between knowledges with the attitudes of female high school students in Badung toward cervical cancer and the correlation between attitudes with HPV vaccination participations. The research method used was cross-sectional analytic with samples of 100 female students in SMAN 1 Kuta, SMK Pariwisata Triatma Jaya and SMAN 1 Kuta Utara. Data were collected by personal interviewed using prepared questionnaires and analyzed using Somersd correlation test. Results of the studies showed, that students categorized as moderate knowledges were 65%, students with good attitudes 72%, and 64% of them received HPV vaccinations. There was significant correlation between knowledges and attitudes (p = 0.036, r = 0.214), and no significant correlation between attitudes and HPV vaccination participations (p = 0.207). For the government of Badung, it is hope to continue free HPV vaccination program and for the local health centre to hold counseling about HPV for female senior high school students routinely. Keywords: cancer, knowledges, attitudes, HPV vaccination. PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

278	Biology	ABS-481
Scientific Teaching Materials Based Structured Inquiry Assisted by Animation Media Jamiatul Hasanah (a*), Jamaluddin (b), Gito Hadi Prayitno (b) 1.Postgraduate Student of Science Education, University of Mataram. Jalan Majapahit No 62, Lombok, Indonesia. *jamihasanah94[at]gmail.com 2.Science Education, Postgraduate Program, University of Mataram. Jalan Majapahit No 62, Lombok, Indonesia. Abstract Abstract. The Problem of science learning at schools usually relates to teaching materials. Teaching materials used as study guides are usually not in accordance with the character and environment of students. This research aims to develop scientific teaching materials based structured inquiry assisted by animation media. The development procedure in this research uses the Dick & Carey model which has 10 development stages namely asses need to identify instructional goal, conduct instructional analysis, analyze learner and contexts, write performance objectives, develop assessment instruments, develop and select instructional material, develop instructional strategy, design and conduct formative evaluation of instruction, revise instruction, design and conduct summative evaluation. The quality of developed product was assessed through feasibility test by two experts and one teacher as a user. Feasibility aspects that are assessed are the content, presentation, language, graphics, and learning media. The feasibility percentage of each aspect was 84%, 88%, 85%, 79% and 83%. The average result of the feasibility presentation value is 83,8%. In conclusion, scientific teaching materials based structured inquiry assisted by animation media is very feasible to be applied in the learning process in junior high school. Keywords: scientific teaching materials, structured inquiry, animation media PermaLink | Plain Format | Corresponding Author (Jamiatul Hasanah)

279	Biology	ABS-993
Factors associated with heigh of medical student of University of Warmadewa Made Cantika Kumara Dipa, I Gusti Ngurah Putu Sana Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Short body height will be a problem if the prevalence of abbreviation is high, which is 30-39% and very high such as ≥40%. In Indonesia the prevalence of short women >18 years in 2013 amounted to 41.6%. Short women tend to have risky pregnancies and give birth to short children as well. Short children have a greater risk of developing degenerative diseases and tend to have lower cognitive abilities that will impact on labor productivity and the economic condition in their adulthood. The purpose of this research is to know the correlation between age of menarche, birth weight and height of parent with the height of student at Faculty of Medicine and Health Science Warmadewa University. The method used was analitic cross sectional, about 66 samples were chosen using systematic random sampling technique and the data will be analyzed by correlation test of contingent coefficient with p <0,05. The results of this study were found to have a significant correlation with height of female students FKIK Unwar is birth weight (p = 0,016, C = 285) and height of parent (father’s height: p = 0,031, C = 0,256; mother’s height: p = 0,045, C = 240). While the age of menarche is not have a significant correlation with height of female students FKIK Unwar (p = 0,084, C = 208). Based on this study, the height of the parent body and the birth weight has a weak positive correlation with height of female students FKIK Unwar, while the age of menarche does not have a significant correlation with height of female students FKIK Unwar Keywords: height, age of menarche, birth weight and height of parent PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

280	Biology	ABS-738
Path Analysis of Hemoglobin level and Working Memory with Learning Outcome in Cell Subject Matter Rusdi(a)*, Sri Rahayu (a), Dwi Ayu Oktaviani (a) a) Biology Education, Faculty of Mathematics and Science, State Univesity of Jakarta Jalan Rawamangun Muka RT 11 RW 14, Jakarta Timur, Indonesia, 13220 *rusdi[at]unj.ac.id Abstract The purpose of this study was to identify direct and indirect effect from hemoglobin level and working memory to learning outcome of cell subject matter. The samples of this research were 52 male students. This research was conducted at SMAN 22 Jakarta in July-August 2018. The level of hemoglobin was measured by cyanmethemoglobin method from HemoCue device, working memory was measured by questionnaire, and learning outcome by achievement test. Data was analysis with path analysis. The results showed that there was direct effect from hemoglobin level to working memory, direct effect from hemoglobin levels to learning outcome, and direct effect from working memory to learning outcome. There was indirect effect from hemoglobin levels to learning outcome through working memory. The effects were highly significant. It can be concluded there were direct effect from hemoglobin level and working memory to learning outcome. Keywords: Hemoglobin, learning outcome, working memory PermaLink | Plain Format | Corresponding Author (Dwi Ayu Oktaviani)

281	Biology	ABS-994
Determinants Of Nursing Quality Sleep Disorder In Tabanan General Hospital in 2018 Ni Kadek Linda Mery Sandhi, Saktivi Harkitasari Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Sleep is one of the primary needs of the basic requirement for human survival. Sleep disorders can cause some effect in humans. It is estimated that 60-80% of workers with shift work system have impaired sleep quality. This results in a decrease in work productivity and an increase in the occurence of accidents. This study aims to determine the determinants of sleep quality disorders nurses in Tabanan General Hospital. This study used cross sectional analytical observational method by conducting questionnaire based interview. The study population was nurses in Tabanan General Hospital with sampling using consecutive sampling technique with samples of 94 people. The results of this study found that factors that have a statistically significant relationship with the quality of sleep is married / have a marital status (PR = 5,53, IK 95%: 1,50-20,39, p <0,05), nurses with tollders (PR = 3,88, IK 95%: 1,28-11,71, p < 0,05) and work system shift (PR = 19,46, IK 95%: 2,48-152,69, p < 0,05). While the factors that have not statistically significant relationship were sleeping environment (PR = 2,06, IK 95%: 0,72-5,91, p=0,17) and coffee consumption in high quantities (PR = 1,41, IK 95% : 0,53-3,73, p=0,48). Based on the result of research, it is found that marital status, toddler ownership and work system are the determinants of nursing sleep quality disorder in Tabanan General Hospital. Keywords: sleep quality disorder, determinants, nurses PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

282	Biology	ABS-995
Profile of abortion in West Lombok I Putu Arya Winata, Saktivi Harkitasari Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Abortion is the end of pregnancy before the age of 20 weeks. Abortion is commonly happened in pregnancy. Abortion can be harmful to the mother and can cause death. Maternal death rate in Indonesia is currently still quite high, reaching 359 per 100,000 live births. The risk factors of abortion are the amount of the workload and maternal age at pregnancy and parity. In this study, the factors that become the determinants in the occurrence of abortion are found. The research was conducted in West Lombok regency, West Nusa Tenggara with cross sectional method and sampling technique using systematic random sampling, there were 64 samples. The data collection is done by distributing questionnaires which are then analyzed by using computer application. The result of this research shows that the majority of abortion patients are at the age of 20-30 years with 17 respondents (68.00%), in education category, it shows that the majority of abortion patients in primary, junior high, and senior high school are 26 respondents (57.78%). The occupation category also shows more abortion patients in the heavy workload with 22 respondents (56.40%) and in parity category, the majority of patients which experienced abortion in two times parity are 18 respondents (54.55%). It is hoped that health workers will prioritize protective measures and adequate service approach, besides it is expected that the health service center develops a supportive health promotion program Keywords: Abortion, Determinants, West Lombok regency PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

283	Biology	ABS-740
DEVELOPING SMARTPHONE BASED LABORATORY MANUAL AS A LEARNING MEDIA Ade Suryanda (a), Nurmasari Sartono (a), Halimatus Sadiyah (a) (a) Biology Education Department, State University of Jakarta, Jalan Rawamangun muka, Jakarta 13220, Indonesia. *adesuryanda[at]yahoo.com Abstract The development of learning media is inseparable from the rapid development of technology. The purpose of this study was to develop a learning media for biology practicum guides in the form of smartphone applications. This research was conducted using the ADDIE model (Analysis, design, development, implementation, and evaluation). The media feasibility test obtained an assessment of 90.28%, material experts 87.75%, biology teachers 91.37% and 88.46% students with very good interpretations. The increase in psychomotor ability test obtained a gain value of 0.49, which means there is an increase in psychomotor abilities of students with a moderate category. The conclusion obtained is that the application of the biology practicum guide has been successfully developed and is suitable for use in learning. Keywords: smartphone, laboratory manual, learning media. PermaLink | Plain Format | Corresponding Author (Halimatus Sadiyah)

284	Biology	ABS-996
Relationship Between Junk Food Consumption With Obesity In Children Aged 9-12 Years In West Denpasar District Kadek Anggiswari Pradnya Angela, Anak Agung Ayu Lila Paramasatiari Faculty of Medicine and Health Sciences, University of Warmadewa Abstract The increases prevalence of obesity is due to the instant diet of children cause of globalization. The aim of this study was to determine the relationship between junk food consumption with child obesity. This study was conducted on 175 pupils which age 9-12 years old and has parents with no history of obesity in West Denpasar District using analysis cross sectional approach. Obesity determined as BMI by age more than Z score 2 while junk food consumption determined as consuming low-nutrient foods contained fat and soft drinks at least once a week. The collected data analyzed with SPSS 21 using Chi-Square test for relationship of junk food consumption and child obesity whereas multiple logistic regression analysis to controlled the influences of variables parents’s education, parents’s income and child’s physical activity. Prevalence of obesity was found 18,3% which 21 pupils found with obesity was consuming junk food, while 11 pupils found with obesity wasn’t consuming junk food. The result was showed as follow: relationship between junk food consumption with child obesity was positive significance (p <0,05), OR 2,56 and IK 95% 1,15-5,72. After controlled with another variables, the relationship still significant with Adjusted Odd Ratio 0,32. It can be concluded that there was relationship between junk food consumption and child obesity. Keywords: junk food, risk factor, obesity PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

285	Biology	ABS-997
The Correlation between Breakfast with Level of Concentration in Elementary School’s Pupils in Denpasar City 2017 I Komang Medy Wedhangga, A.A.A. Lila Paramasatiari Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Breakfast is a meal in the morning, shortly after waking up to 10 (ten) AM. This activity has a purpose to fulfill the body nutrition in the morning before starting the activity. Breakfast is considered important for a pupils which sometimes has a very solid activity in school. In addition, in the academic process pupils need to be in a healthy body condition and have good attention and concentration to catch all the lessons in school. The aim of this research was to find out the correlation between breakfast with the level of concentration in elementary school’s pupils in Denpasar City 2017. The research design was descriptive analytical through cross-sectional approach which was analyzed using Chi-Square statistical test by Statistical Package for the Social Sciences for Windows 16.0 (SPSS) program. The research data was primary data gathered with questionnaire form. The amount of the subjects were 96 persons which was selected using multistage cluster random sampling method to an elementary school’s pupils in Denpasar City. The percentage of non-breakfast subject was 35 persons (36,5%) and the breakfast subject was 61 persons (63,5%). The percentages of Concentrations level of breakfast pupils were divided in three categories which were Low (11,48%), Medium (65,57%) and High (22,95%). The result was showed that there was a significant relationship (p<0,05) between breakfast with the level of concentration. The conclusions could be drawn that there was a positive mild relationship between the two variables. It was recommended that this research can contribute as a source of information and evaluation for the development of science in the future. Keywords: Concentration, Breakfast, Pupils, Elementary School, PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

286	Biology	ABS-998
Association between physical fitness with dysmenorrhea among junior high school student Ni Putu Ana Novita, I Made Sarmadi Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Dysmenorrhea or stomach pain every menstruation is common symptom of adult women. Primary dysmenorrhea usually occurs about 2 years after menarche and which age about ±20 years. There are many incidence of dysmenorrhea. Physical fitness is one of the factor that affect the occurrence of dysmenorrhea. This aims of the study to determine the percentage of dysmenorrhea and correllation to physical fitness. The method of this study was csross-sectional. The study was conducted 71 female people of SMP Negeri 3 Gianyar. The physical fitness was measured using Tes Kesegaran Jasmani Indonesia (TKJI) and dysmenorrhea data using questionnaire. The data was analysed using Somersd tes. The result was follow the physical fitness with very low category 6 (8,5%), low category 36 (50,7%), average category 25 (35,2%) and good category 4 (5,6%). The percentage of dysmenorrhea was 56 (78,9%) and not dysmenorrhea was 15 (21,1%). The result of this study indicated there was significan correllation between physical fitness with dysmenorrhea (p<0,005). Keywords: Dysmenorrhea, Physical Fitness PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

287	Biology	ABS-743
Computer Assisted Instruction (CAI)-Based Learning: Instructional Need for Pupils’ Conceptual Understanding of Water Cycle Shofiyatun Nurlaili (1), Sapriya (2) 1Department of Primary Education, Universitas Pendidikan Indonesia, Jl.Dr. Setiabudi 229, Bandung 40154, Indonesia 2Department of Civic Education, Universitas Pendidikan Indonesia, Jl. Dr. Setiabudi 229, Bandung 40154, Indonesia Abstract This research was conducted based on the lack of students conceptual understanding of ecology and the environment related to water cycle material. This material is considered important to be analyzed because it relates to life that occurs in the community. The purpose of this study was to give alternative an instructional need for students conceptual understanding of the water cycle that uses a Computer Aided Instruction (CAI) based on learning. The multimedia-based CAI application used by the researcher to understand the concept of the water cycle is implemented on laptops or desktop computers used Windows operating system. The subjects of this research were three students average 11-13 years old. The instruments used were observation, mini-interviews, and tests. The results of this study indicate that understanding students concepts of the water cycle are still lacked and many misconceptions can be seen from the results of student work when drawing water cycles. Furthermore, the use of CAI based learning is needed to help students conceptual understanding of the water cycle material through multiple-choice questions and drawing the water cycle correctly. Keywords: Computer Aided Instruction, understanding students concepts, water cycles PermaLink | Plain Format | Corresponding Author (Shofiyatun Nurlaili)

288	Biology	ABS-1001
Level Of Knowledge, Attitude, And Behavior Against Rheumatic Disease In Samplangan Urban Village Gianyar District Made Nanda Hadi Wiguna, I Wayan Darwata Faculty of Medicine and Health Sciences, University of Warmadewa Abstract The elderly population in Indonesia has increased significantly as indicated by the increasing average life expectancy of Indonesians in the last decade , from 69.1 years (2005) to 70.1 years (2015). In Indonesia, rheumatism ranks 2 of the top 10 health problems of the elderly. Gianyar District Health Office report in 2013, mentioned that the number of rheumatic visits was ranked 3rd out of 10 major diseases at health care. This study aims to determine the level of knowledge, attitudes and behavior of elderly against rheumatic diseases in Samplangan Urban Village, Gianyar District. This study uses descriptive quantitative design with cross-sectional approach.Respondents in this study of 106 elderly are recorded in Banjar Bukit Batu and Selat, Samplangan Village Gianyar. In this study the sample was selected using simple random sampling. Data collection did by direct interview and using questionnaires that has been valid to the respondent. The results obtained that generally the level of knowledge of the elderly against rheumatic disease is still not good. As for the attitude and behavior of the elderly against rheumatic diseases are already in good category. Therefore expected that Samplangan Urban Village can provide further information or counseling about rheumatism to improve the knowledge and health status of the elderly. Keywords: Elderly, Rheumatism, Level of Knowledge, Attitude, Behavior PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

289	Biology	ABS-746
LABORATORY MANUAL BASED ON SMARTPHONE APPLICATION IN INCREASING STUDENTS PSYCHOMOTORIC LEARNING OUTCOME Nurmasari Sartono(a)*, Ade Suryanda (a), Halimatus Sadiyah (a) (a) Biology Education Department, State University of Jakarta, Jalan Rawamangun muka, Jakarta 13220, Indonesia. *nurmasari_sartono[at]yahoo.com Abstract The limited information that is only in the form of text and images in the practicum guide that already exists causes various obstacles experienced by students in biology labs. The smartphone application comes with the potential to combine information in the form of text, images, videos and quizzes as a medium for student learning. This study aims to see the effect of the use of smartphone biology practical guide applications on the ability of science processes of students using quasi-experimental methods with pre-post-test control group design research design. The conclusion obtained is that the use of biological practicum guidance application has a positive influence on students scientific process abilities. Keywords: laboratory manual, smartphone, psychomotoric, outcome. PermaLink | Plain Format | Corresponding Author (Halimatus Sadiyah)

290	Biology	ABS-748
Benthos Population in Seagrass Ecosystem at Bira Island Kepulauan Seribu, Jakarta, Indonesia Ratna Komala (a*), Mieke Miarsyah (a), Ratna Dewi Wulaningsih (a) a) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia Abstract One group of organisms that can be used as bioindicators of water quality is benthos, these organisms are spread over several habitats and also in coastal areas, one of which is in the seagrass ecosystem on Bira Island, located in the Kepulauan Seribu Jakarta. The aim of this study was to determine the dynamics of benthic population as an illustration of the environmental conditions on Bira Island for 6 months of observation. The method used in this study was descriptive with survey design. Data collecting by purposive sampling with the transect method at 3 observation stations. The data were analyzed descriptively through the calculation of abundance, diversity and dominance. The results of the study identified 23 species of benthos included in the 3 phylum with the largest to lowest composition represented by molluscs, arthropods and echinoderms phylum. The benthic population fluctuates and shows a pattern of decreasing trends every month during observation. The abundance of the highest species of each phylum is represented by Turicula javana (Mollusc), Portunus sp (Arthropods) and Holothuria atra (Echinoderms. The Species Abundance based on the observation station shows that the highest abundance is at station 1, followed by station 2 and the lowest is station 3. Benthos diversity at each station is in the moderate category, and there is no dominant species Keywords: Benthos; Bira Island; Seagrass; Kepulauan Seribu PermaLink | Plain Format | Corresponding Author (Ratna Komala)

291	Biology	ABS-1004
Application of biochar and compost to changes in physical and chemical properties of soil and corn yield on dry land I Gusti Lanang Agung Alit Adi Putra, Yohanes Parlindungan Situmeang *, Made Sri Yuliartini, and I Gusti Bagus Udayana Agriculture Faculty, Warmadewa University, Jl. Terompong 24 Tanjung Bungkak, Denpasar-Bali, 80235, Indonesia * Email: ypsitumeang63[at]gmail.com Abstract The purpose of this study was to determine the effect of biochar and compost fertilizer and their interactions on changes in the physical and chemical properties of soil and corn yields on dry land. This experiment used a randomized block design with factorial patterns. The treatment consists of two factors, namely: the dose of biochar and compost. The first factor, the dose of biochar consists of 4 levels, namely: 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, 15 tons ha-1. The second factor, the compost dosage consisted of 2 levels: 0 tons ha-1 and 20 tons ha-1. The highest dry shelled seed per ha was obtained from the treatment of 10 tons ha-1 biochar which was 9.21 tons which increased by 35.56% compared to the lowest dry shelled seed per ha obtained from the treatment without biochar which was 6.79 tons . The treatment of compost doses of 20 tons ha-1 gave the highest value of dry shelled beans per ha, which was 8.73 tons, increasing by 16.58% compared to the lowest yield of dry shelled seeds per ha, which was 7.49 tons in the treatment without compost. Keywords: bamboo biochar, compost, soil physical and chemical properties, corn plants PermaLink | Plain Format | Corresponding Author (Yohanes Parlindungan Situmeang)

292	Biology	ABS-494
ACCURACY OF MYOGLOBIN mRNA IN THE BRAIN OF NORMAL RATS (Rattus novergicus) USING PCR REAL TIME TECHNIQUE Rini Puspitaningrum1*, Apriliana L.F1, DiahAsriyanti1, Erfan Handoko2 1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia 2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia Abstract Myoglobin is a cytoplasmic hemoprotein with an important role of maintaining oxygen homeostasis of tissues. The brain needs quite a high amount of oxygen to carry out its metabolism. Therefore, this study was conducted to detect the expression level of myoglobin mRNA in the brain of normal rats (Rattus novergicus).The study was conducted at the Biochemistry and Molecular Biology Laboratory of the Biology Major of the Faculty of Mathematics and Natural Sciences, UNJ from December 2010 to February 2011. Through 1.5% agarose gel electrophoresis, results show that the myoglobin band of 114bp was detected in the brain of normal rats (Rattus novergicus). Due to the positive results, there is a huge possibility that the myoglobin expressed in the brain of rats (Rattus novergicus) would be altered into protein during environmental conditions of low oxygen concentration. Keywords: myoglobin mRNA, normal, brain, Rattus novergicus. PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

293	Biology	ABS-495
DETECTION OF MYOGLOBIN PROTEIN IN GREEN SEATURTLE (Chelonia mydas) ORGANS WHICH CONSUMEHIGHLY CONCENTRATED O2 USING SPECTROPHOTOMETRY AND IMMUNODOT BLOTTECHNIQUES Rini Puspitaningrum1*, Khadijah Dinda Y1, Erfan Handoko2 1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia 2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia Abstract Myoglobin is a hemoprotein with the important roleof being the binding and storage agent of oxygen in tissues. That is why it is important to study the existence of myoglobin in hypoxia tolerant animals, such as the green sea turtle (Chelonia mydas). This study aims to detect myoglobin protein in green sea turtle (Chelonia mydas) organs which consume highly concentrated O2 using spectrophotometry and immunodotblottechniques. In this study, the myoglobin protein of the heart, brain, and liver was analyzed using spectrophotometry at ƛ503nmand immunodot blot techniques with the antibody – anti myoglobin of the green sea turtle (Chelonia mydas). The results obtained is that the myoglobin protein is detected in all organs. The lungs are the organ with the lowest concentration of myoglobin protein from all the test organs. Based on the study results, it can be concluded that genetically, the green sea turtle (Chelonia mydas) would evolve as a tolerant animal toward hypoxia. Keywords: myoglobin, hypoxia tolerant, green sea turtle (Chelonia mydas), organs which consume highly concentrated O2 PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

294	Biology	ABS-496
DETECTION OF MYOGLOBIN mRNA EXPRESSION IN THE LIVER OF NORMOXICRATS (Rattus novergicus) Rini Puspitaningrum1*, Nur Upik1, Erfan Handoko2 1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia 2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia Abstract Myoglobin is an intracellular protein which has a vital role in maintaining oxygen hemostasis within living tissue. This protein has the ability to bind and send oxygen to mitochondrial membrane to enable transduction of energy. The liver is the biggest organ in the bodyand has complex functions. Based on the complex functions, liver needs quite a big amount of oxygen, so the metabolism process can be performed well. This study aims to detect mRNA expression in the liver of rats (Rattus novergicus) in normoxia. The study was conducted at the Biochemistry and Molecular Biology Laboratory of the Biology Major of the Faculty of Mathematics and Natural Sciences, UNJ. This study utilized quasi-experiment method. The study results show that myoglobin mRNA is expressed in the liver of rats (Rattus novergicus) in normoxia on 114 pb. This result can create a new paradigm towards myoglobin gene that this gene might be a constitutive gene. This information can be the basis that living tissues have strategies for mechanism of adaptation towards low oxygen environment by increasing the expression of myoglobin in the tissue. Keywords: myoglobin mRNA, liver, normoxia, Rattus novergicus PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

295	Biology	ABS-752
The Life Cycle of Great Mormom Butterfly (Papilio memnon memnon) in Captivity Ratna Komala (a*), Ade Suryanda (a), Sri Yuliasih Wiyati (b) a) Department of Biology , Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta , Indonesia b) Student of Magister Degree, Departement Biology, Bogor Agriculture University, Bogor Indonesia Abstract Papilio memnon memnon is one of the butterflies groups of the Papilio clan in West Java, Indonesia. This study aims were to determine the morphology, behavior, and life cycle of the P. m memnon butterfly. The research method used is descriptive with survey techniques and literature studies. This study aims to determine the morphology, behavior, and life cycle of the P. m memnon butterfly. The research method used was descriptive with survey techniques and literature studies. Data about morphology, duration of life, and behavior at each development phase during the P. m memnon butterfly life cycle were analyzed descriptively based on direct observation. The results showed the egg phase of P. m. memnon takes about 7-8 days to the phase instar1 of the larvae. The average total time needed in the larval phase is around 23 - 45 days to the pupa phase. The Pre-pupa phase lasts 1-2 days, while the pupa phase lasts 2-3 weeks. P. m. memnon performs the larval phase as many as 5 stages of instar. Food eaten by first phase instar 1 of larvae is the eggshell, which is useful for insect growth and helps eliminate evidence of eggs from parasitic attacks. At each phase of development, the larvae eat the outer skin to obtain additional energy to support growth. Keywords: Life cycle; larvae; pre-pupae; imago; Papilio memnon PermaLink | Plain Format | Corresponding Author (Ratna Komala)

296	Biology	ABS-497
MYOGLOBIN EXPRESSION IN THE KIDNEY AND LUNGS OF BRAZILIAN TURTLE (TrachemysScriptaElegans) Rini Puspitaningrum1*, Apriliana1, Kheista Japri 1, Erfan Handoko2 1Dept. of Biology, Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia 2 Dept of Physics. Universitas Negeri Jakarta. Jalan Rawamangun Muka 13220. Jakarta Indonesia Abstract Myoglobin has an important role in maintaining the oxygen homeostasis within living tissues. Therefore, the existence of myoglobin is very important to be studied in hypoxia tolerant animals, in this case the Brazilian turtle (Trachemysscriptaelegans). This study aims to detect myoglobin expression in the heart, kidney and lung of Brazilian turtle using the myoglobin primers Myoturtle_F2 and Myoturtle_R1. This study was performed using the RNA total of the heart, kidney, and lung of Brazilian turtles, and using various primer concentrations and annealing temperatures. This study was conducted at the Laboratory of Biochemistry and Molecular Biology of FMIPA UNJ from June to August of 2010. Through 2% agarose gel electrophoresis, a myoglobin band of 114 pb had been detected in the kidney of normoxic Brazilian turtle. In the lung of the Brazilian turtle, myoglobin was not detected which might be caused by the low myoglobin concentration within the organ. Keywords: myoglobin, hypoxia tolerant, Brazilian turtle (Trachemysscriptaelegans) PermaLink | Plain Format | Corresponding Author (Rini Puspitaningrum)

297	Biology	ABS-758
Characteristics of Emotional Dysregulation in People with Allergic Rhinitis Ambar Sulianti, Aghni Aghnia, Waode Zahara Adzkiatun Nisa UIN Sunan Gunung Djati Abstract Allergic rhinitis decreases the sufferers productivity so that it affects emotions. This study aims to examine emotional dysregulation in people with allergic rhinitis. This research is a quantitative study by analyzing emotional regulation instruments for 60 productive age allergic rhinitis sufferers. Statistical analysis of Kruskal walls and post hoc Mann Whitney between aspects showed that there were 3 groups of emotional regulation levels. The results showed that patients with allergic rhinitis had low self-control and confidence. Respondents have moderate emotion managing, emotion modify, and concentration levels. There were no difficulties in evaluating emotions in the research subjects. Allergic rhinitis causes an embarrassed person to face other people when sneezing and snot. The condition of sudden sneezing and the urge to itch the nose makes it difficult for respondents to control themselves. However, patients with allergic rhinitis can still concentrate on their work and can manage and modify their emotions. Keywords: allergic rhinitis, regulation of emotions, confidence, concentration, emotional modification. PermaLink | Plain Format | Corresponding Author (Ambar Sulianti)

298	Biology	ABS-248
The Effects of Composition Cocopeat and Rice Husk Charcoal as Grow Media on The Growth of Kailan Plant (Brassica oleraceae Var. Achepala) with Drip Hydroponic System Ahmad Taofik (a), Budy Frasetya (a*), Ruby Nugraha (a), Adjat Sudrajat (a) a) Agrotechnology Department, UIN Sunan Gunung Djati Bandung, Jalan A.H. Nasution No. 105 Bandung 40614, Indonesia *)budyftq1682[at]gmail.com Abstract The drip Hydroponics system has a weakness which at a high evapotranspiration condition cant fulfill plant water requirement properly. Improve the composition of plant growth media with cocopeat and rice husk charcoal as a solution to increase Kailan Plant Growth. This research aims to study the effect of a ratio between cocopeat and rice husk charcoal as plant media on the growth of Kailan Plant with drip hydroponic system. The research was conducted at Jatinangor-Sumedang-West Jawa from November to December 2017. The method of this research was use completely randomized design with five treatment (A=Cocopeat 100%; B=Cocopeat 75%+ Rice Husk Charcoal 25%; C= Cocopeat 50% + Rice Husk Charcoal 50%; D= Cocopeat 25% + Rice Husk Charcoal 75%; E= Rice Husk Charcoal 100%), and five replication. The growth indicator in this research is plant height, number of leaves, leaf area, fresh plant weight, and shoot-root ratio. The ratio of cocopeat and rice husk charcoal as plant growth media affect the plant growth of kailan. The result showed that composition grows media Cocopeat 75% + Rice husk charcoal 25% affect plant height, fresh plant weight, and shoot-root ratio. Keywords: Drip hydroponic system, Composition plant grow media, Cocopeat, Rice husk charcoal PermaLink | Plain Format | Corresponding Author (Budy Frasetya)

299	Biology	ABS-504
Effect of Problem Based Learning Models on Student Skills in Conducting Validity and Reliability Test of Objective Question Ratna Dewi Wulaningsih Universitas Negeri Jakarta (UNJ) Biology Education Study Program, Faculty of Mathematics and Natural Sciences, Kampus A, Gedung Hasjim Asjarie Lt. 9. Jl. Rawamangun Muka Jakarta Timur, 13220, Indonesia Abstract Testing the validity and reliability of objective questions on PPEP (Perencanaan, Pengelolaan, dan Evaluasi Pembelajaran/Learning Planning, Management, and Evaluation) courses which is carried out at the Biology Education Program FMIPA UNJ demands thorough attention, considering the lecture material in this course is important to provide adequate knowledge for the students as prospective education graduate. Problem Based Learning Model is a cooperative learning model that focuses on student group work in the form of small groups, which consists of four to six people. Students in groups will work together in a positive way and become responsible independently. Data collection using pretest and post-test completed with documentation. The results of this study shows that there is an influence of the problem-based learning model on students skills in testing the validity and reliability of objective questions on PPEP courses. Keywords: validity, reliability, objective question, problem-based learning model PermaLink | Plain Format | Corresponding Author (Ratna Dewi Wulaningsih)

300	Chemical Engineering	ABS-539
ISOTHERMAL VAPOR-LIQUID EQUILIBRIUM MEASUREMENT OF ISOBUTANOL + ISOOCTANE/N-HEPTANE BINARY MIXTURES AT TEMPERATURES RANGE OF 303.15-323.15 K Ardika Nurmawati, Gede Wibawa Department of Chemical Engineering, Faculty of Industrial Technology, Sepuluh Nopember Institute of Technology (ITS), Kampus ITS Sukolilo Surabaya, 60111, Indonesia Abstract The addition of alcohol as an oxygenated compound, affect the vapor pressure of gasoline. In this study, the vapor pressure of isobutanol – isooctane/n-heptane blends were investigated. Vapor-liquid equilibrium data for the binary systems of isobutanol + isooctane/n-heptane were measured at low temperatures ranging from 303.15 to 323.15 K. The measurement was carried out using simple quasistatic ebulliometer. The apparatus validated by comparing the vapor pressure of pure isobutanol, isooctane, and n-heptane with the published data and average absolute deviation (AAD) obtained was less than 0.83%. The vapor pressure of the binary mixtures correlated with the Wilson, Non-random two-liquid (NRTL), and Universal quasichemical (UNIQUAC) models. The AAD for isobutanol + isooctane system were 1.54%, 1.44%, 1.71% respectively and for isobutanol + n-heptane system were 1.73%, 1.65%, 1.99% respectively. The vapor pressure from the experiment for all systems shows a positive deviation from Raoult’s Law that indicates a nonideal solution. Keywords: Vapor pressure, Isobutanol, Isooctane, N-heptane PermaLink | Plain Format | Corresponding Author (Ardika Nurmawati)

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