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241	Biology	ABS-965
Level of Knowledge, Attitude, and Community Behavior Against Alcoholic Beverages in Abiansemal Village, Badung Igam Aditya Prasada, I Wayan Darwata Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Alcoholic beverages are beverages containing alcohol (C2H5OH) which fermented or distilled from carbohydrates. Alcohol can trigger disorders such as cancer, cardiovascular disease, neuropsychiatry, gastrointestinal tract, infection, injury, and neonatal abnormalities. About 38.3% world population aged 15 years and above consumed alcohol, while in Indonesia 4.6%. A quarter of the consumption (24.8%) is illegal, which is homemade alcohols that are produced or sold out of government control. Therefore, the research was conducted with the aim to know the level of knowledge, attitudes, and behavior of people against alcoholic beverages. This research was designed as a quantitative descriptive research with cross sectional approach. Sampling used was multistage random sampling technique, the number of samples were 96 obtained by the formula. The results of this study indicate that the respondents knowledge level is mostly good (67.7%), response attitude is mostly good (63.5%), and the behavior of alcoholic drinkers is more than half (56,1%). Active role of health workers is needed to provide information on alcoholic beverages and the government needs to provide oversight and firm action in the circulation of alcoholic beverages. Keywords: Alcohol, Alcoholic Beverages, Level of Knowledge, Attitude, Behavior PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

242	Biology	ABS-966
Biostratigraphic Analysis Based on Planktonic Foraminifera from Well X in North Sumatra Basin Dewi Syavitri, Romy Setiawan, M. Ali Jambak Geology Department Faculty of Earth and Technology Trisakti University Abstract The research area is in the X Well which located in the North Sumatra Basin. There have been several studies conducted in the area, but there is still very little information related to the age of the Formations in the basin. A total of 128 sample are used for lithostratigraphy analysis, in which 33 samples were analyzed further for the Foraminifera biostratigraphy study from the X Well at a depth of 6090 – 9919 feet. From the result of the analysis, 38 species from 16 genera successfully identified, as well as 5 datum species, namely Globigerinoides sicanus, Orbulina suturalis, Globorotalia menardii, Globorotalia plesiotumida dan Globorotalia tumida tumida. From the lithology data, three (3) lithostratigraphic unit successfully identified, namely the calcareous claystone, very fine calcareous sandstone and fine calcareous sandstone unit which is estimated to be comparable to the Baong, Keutapang and Seurula Formation. From the result of the study it’s concluded that the lithostratigraphic units found in the study area have the age range of N7-N18 or late part of the Early Miocene to the late part of the Late Miocene. Keywords: Biostratigraphy, planktonic foraminifera, North Sumatra Basin. PermaLink | Plain Format | Corresponding Author (Dewi Syavitri Husein)

243	Biology	ABS-968
Application of Lake Sludge and Natural Phosphate on Spinach Plant Cultivation (Amaranthus tricolor L.) Ida Bagus Komang Mahardika (a*), I Gusti Made Arjana (a), Evaristus Odi Nango (a) a Department of Agrotechnology, Faculty of Agriculture, Warmadewa University, Denpasar, Indonesia * Email: gusmahardika62[at]gmail.com Abstract Lake sludge and natural phosphate can be applied to the cultivation of various plants, but have not been applied to spinach cultivation. The aim of the study was to determine the effect of Lake Sludge dosage and Natural Phosphate dosage on growth and yield of unripe spinach. The experimental method with factorial RCBD used on this research. The results showed that the interaction between treatment of Lake Sludge (D) and Natural Phosphate (P) dosages had no significant effect on all observed variables. The Lake sludge dose of 150 g per polybag gives total oven dry weight per plant of 8.07 g or 61.1% higher compared to a dose of 0 g per polybag (D0). Natural Phosphate treatment has a very significant effect on the total oven dry weight variables per plant. Natural Phosphate doses of 40 g per polybag provide total oven dry weight per plant of 7.31 g or 39.5% higher than a dose of without Natural Phosphate (P0). Both of the regression analysis between the dosages of Lake Sludge and Natural Phosphate with total oven dry weight per plant there is a linear relationship. Keywords: Lake Sludge; Natural Phosphate; spinach PermaLink | Plain Format | Corresponding Author (Ida Bagus Komang Mahardika)

244	Biology	ABS-713
Correlation Study of Physical Activity and Menstruation Cycle on Biology College Students Yulilina Retno Dewahrani dan Annisa Luthfiani Universitas Negeri Jakarta email: yulilinaretno[at]yahoo.co.id Abstract The main gynecological problems among women, especially adolescents, are disorders of the menstrual cycle. Menstruation is the decay of the uterine wall which causes bleeding that is influenced by hormones, occurs repeatedly and forms cycles. The menstrual cycle is influenced by hormonal, psychological factors such as anxiety, depression, and stress, and lifestyle such as nutrition and physical activity. This study aims to determine the positive relationship between physical activity and the menstrual cycle in female college students of Biology, Jakarta State University. The study was conducted at the Jakarta State University in October-November 2018. The method used was descriptive method with correlational studies. The number of research samples is 177 female college students. The prerequisite test results show that data were normally distributed and homogeneous. The results of hypothesis testing indicate there is a positive relationship between physical activity and the menstrual cycle, physical activity provides a considerable contribution to the menstrual cycle. The conclusion of this study is that there is a positive relationship between physical activity and the menstrual cycle. Keywords: college student, menstrual cycle, physical activity PermaLink | Plain Format | Corresponding Author (Yulilina Retno)

245	Biology	ABS-969
The Level of Knowledge, Attitudes, and Behaviour of the Community towards Alcoholic Beverages in Abiansemal Village, Badung Igam Aditya Prasada1, I Wayan Darwata2 Universitas Warmadewa Abstract Abstract Alcoholic beverages are beverages containing alcohol (C2H5OH) which fermented or distilled from carbohydrates. Alcohol can trigger disorders such as cancer, cardiovascular disease, neuropsychiatry, gastrointestinal tract, infection, injury, and neonatal abnormalities. About 38.3% world population aged 15 years and above consumed alcohol, while in Indonesia 4.6%. A quarter of the consumption (24.8%) is illegal, which is homemade alcohols that are produced or sold out of government control. Therefore, the research was conducted with the aim to know the level of knowledge, attitudes, and behavior of people against alcoholic beverages. This research was designed as a quantitative descriptive research with cross sectional approach. Sampling used was multistage random sampling technique, the number of samples were 96 obtained by the formula. The results of this study indicate that the respondents knowledge level is mostly good (67.7%), response attitude is mostly good (63.5%), and the behavior of alcoholic drinkers is more than half (56,1%). Active role of health workers is needed to provide information on alcoholic beverages and the government needs to provide oversight and firm action in the circulation of alcoholic beverages. Keywords: Keywords: Alcohol, Alcoholic Beverages, Level of Knowledge, Attitude, Behavior. PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

246	Biology	ABS-970
Hypertension risk factors among patients visiting Community Health Centre 1 in South Denpasar Kesuma Yudha Anak Agung Gede; Semadha Wayan Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Hypertension becomes an alarming problem in almost all parts of the world, especially developing countries like Indonesia.In Bali Province cases of hypertension in 2014 became the second sequence of the top ten diseases with a total of 114,421 cases.This study describes risk factors associated with hypertension in patients who visited public health center I south of Denpasar. Case control study is used as research design by using 84 samples (42 cases : 42 control). Data are collected through interviews with a questionnaire of IPAQ, food recall and physical examination of blood pressure. Sampling technique is using consecutive sampling. Data analysis was done gradually including univariate analysis, bivariate analysis using chi-square test, and multivariate analysis using logistic regression test in SPSS program. Multivariate analysis indicated an adjusted odds ratio as follows: salt intake ≥ 6 grams of salt per day (OR: 4,718; 95% CI: 1,6248-13,707), family history of hypertension (OR: 4,156; 95% CI: 1,487-11,612) and physical activity (OR; 4,143 95% CI: 1,486-11,551). While the independent variables that are not proven as risk factors for hypertension are gender (OR: 0.817; 95% CI: 0.338-1.974) and age (OR: 1,370; 95% CI : 0,259-7,249).. The results of this study is expected to be an input for public health center I south of Denpasar that the of promotive and preventive programs of disease is needed to reduce the occurrence of hypertension in the work area of public health center I south of Denpasar. Key Words : Hypertension, Risk Factors. Public Health Center. Keywords: Hypertension, Risk Factors. Public Health Center. PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

247	Biology	ABS-971
The Characteristics of Mothers Giving Birth with Sectio Caesarean Delivery at Bali Royal Hospital Denpasar General Hospital Ni Made Safitri Wulandari1, Dewa Ayu Agung Alit Suka Astini2 Universitas Warmadewa Abstract Abstract Caesarean section is a form of childbirth by surgical procedure. The rate of caesarean delivery in Indonesia is quite high, especially in private hospitals such as in Bali Royal Hospital Denpasar. This is influenced among others by age, education level, occupation, parity and indication of the caesarean section. This study aimed to assess the characteristics of mothers who had caesarean section delivery at Bali Royal Hospital Denpasar. This study used descriptive cross-sectional design. The variables studied were age, education level, occupation, parity and indications of the caesarean section. The samples of the study were 100 mothers, selected by systematic random sampling from 1004 mothers in the population. The data used were secondary data obtained from medical records, then analyzed descriptively. The results showed that the characteristics of mothers who had caesarean section deliveries at Bali Royal Hospital Denpasar based on sociodemographic factors were mostly aged 20-35 years (83%), having college education level (45%) and worked as private employment (52%). Meanwhile based on medico-obstetric factors most had nullipara parity (54%) and medical indications (85%), most of which was previous history of sectio caesarea, as which was high as 42.19%. Keywords: Keywords: sleep duration, concentration level, elementary school students. PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

248	Biology	ABS-716
Environmental Literacy of Biology Undergraduate Students in Jakarta: Profile and Comparative Analysis Diana Vivanti Sigit*, Eka Putri Azrai, Desi Nur Setyawati, Ilmi Zajuli Ichsan Biology Education Program, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Indonesia *Corresponding author: dianav[at]unj.ac.id Abstract Environmental Literacy was the ability to interpret and take appropriate action on environmental systems that purpose to maintain, restore and improve the environment. This study aimed to determine the environmental literacy level of biology undergraduate students. The method was used a descriptive method. The three aspects of environmental literacy were cognitive, affective, and action. Data were obtained from 197 undergraduate students on biology science and biology education departments taken from 5 universities in DKI Jakarta. The result showed that the level of environmental literacy was in a very good category in which the cognitive aspects were the highest. Biology undergraduate students were good in the environment understanding but were still poor in the affective and action aspects. Based on the departments, the environmental literacy of students from biology science was higher than students from biology education. Based on gender, the female was higher in environmental literacy than male. In conclusion, the Biology undergraduate students environmental literacy varies from some aspects. This research recommended that institution has an important role to enhance the student environmental literacy, therefore, a student might increase the affective and the action of environmental literacy. Keywords: Biology undergraduate student, education, environmental literacy, environmental quality PermaLink | Plain Format | Corresponding Author (Diana Vivanti Sigit)

249	Biology	ABS-972
The Relationship between duration Hypertension with Cognitive Function in Elderly at Health Care II West Denpasar area Stephany Angelia, Putu Sutisna Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Hypertension is a disease that is often experienced by elderly. According to Joint National Committee (JNC) VII hypertension is a condition in which a person’s systolic blood pressure is ≥ 140 mm Hg and or diastolic blood pressure ≥ 90 mmHg. Hypertension can cause complications, one of which is cognitive dysfunction. In Indonesia, 7-9% of hypertensive patients have cognitive dysfunction. Based on the data of Bali Provincial Health Office (2015), hypertension was second rank among 10 major diseases in patients at Health Centre. Health Centre II West Denpasar has the highest number of hypertensive patients in Denpasar. This study aimed to determine the relationship between duration of hypertension and cognitive function in elderly at Health Centre II West Denpasar area. This study used analytic cross sectional design with 71 elderly respondents ≥ 60 years old selected by Consucutive Sampling from the elderly attending Posyandu (Comprehensive Health Care) program at Health Centre II West Denpasar. Secondary data were used taken from medical records in the Health Centre II West Denpasar. Instrument used in this study was Mini Mental State Examination. (MMSE). Data were analyzed statistically using univariate and bivariate analysis by Chi Square test. The results showed suffered hypertension ≥ 5 year 68.9% respondents with decreased cognitive function, while suffered hypertension < 5 year 38.7% respondents with decreased cognitive function.There was a significant relationship between duration of hypertension and cognitive function in elderly (p = 0,009; r=0,287). Keywords: Cognitive Function, Hypertension, MMSE PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

250	Biology	ABS-973
The Relationship between Diet and Physical Activity with Nutritional Status in Children Aged 9-12 Years in the Primary School 1 of Batannyuh Tabanan Ni Kadek Paula Dewi1, Dewa Ayu Agung Alit Suka Astini2 Universitas Warmadewa Abstract Abstract Nutritional status is the state of the bodys end result of the balance between the nutrients intake and its utilization. Nutritional problems that usually occur are underweight and overweight. In Indonesia, the prevalence of obesity is 18.8% and underweight is 11.2% which occur in children aged 9-12 years. The prevalence of obesity in children of that age in Bali Province is 20%. Various factors can affect nutritional status such as diet and physical activity. The purposes of this study were to determine the correlation of diet and physical activity with nutritional status in children aged 9-12 years. This study used analytic cross sectional design with 60 samples taken from all students of 4th, 5th and 6th grade of SD Negeri 1 Batannyuh Tabanan that fulfilled the inclusion and exclusion criteria. The variables studied were nutritional status as dependent variable and physical activity as independent variables. The instrument used in this study was a questionnaire that had been tested for validity. Data were analyzed with Somersd correlation test. The results showed that most of the respondents had good diet (51,7%), poor physical activity (53,3%), and normal nutritional status (51,7%). There was a significant correlation with mild positive correlation between diet and nutritional status (p=0,025) and between physical activity and nutritional status (p=0,026). Result of this study are expected to be useful both theoretically and practically in order to perform promotive or preventive actions related to nutritional status. Keywords: Keywords: Nutritional status, Children, Diet, Physical activity. PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

251	Biology	ABS-207
The Use of Trichoderma harzianum and Aspergillus niger in Indigofera zollingeriana Fermentation M Puspitasari, I Hadist. T Rohayati, M Royani Universitas Garut Abstract Indigofera zollingeriana is a feed that has high crude fiber content so it is difficult to use for poultry feed. The fermentation process using fungus is expected to overcome this problem. The study aimed to determine the interaction between dose and fungi kinds, with the length of fermentation on the protein and crude fiber content of I. zollingeriana. Another goal is to determine the optimum conditions for fermenting I. zollingeriana. The method used a Completely Randomized Design (CRD) factorial pattern. The first factor consisted of 6 levels of the different fungi dosages, namely 0.1%, 0.2% and 0.3% Trichoderma harzianum; 0.1%, 0.2% and 0.3% Aspergillus niger. The second factor was four levels of the duration of fermentation: 24 hours, 48 hours, 72 hours and 96 hours. All treatments were repeated twice. The experimental data were tested by F test, then Duncans analysis was carried out. The results showed that there was an interaction between dose and fungi kinds with the length of fermentation. The optimal condition for fermenting I. zollingeriana with T. harzianum fungus was at a dose of 0.3% and 96 hours fermentation time. This treatment gave the increasing of protein content and reducing crude fiber content. Keywords: Trichoderma harzianum PermaLink | Plain Format | Corresponding Author (Maryati Puspitasari)

252	Biology	ABS-975
The Relationship between Obesity and Family History with Blood Uric Acid Levels In Patients with Hyperuricemia Aged Over 30 Years at Bangli General Hospital Putu Pradnya Saraswati1, Sagung Putri Permana Lestari Murdhana Putere2 Universitas Warmadewa Abstract Abstract Hyperuricemia is a condition in which there is an increase in blood uric acid level above normal. Some studies have shown more than 70% of Hyperuricemia sufferers are obese and have a family history of hyperuricemia. This study aimed to determine the relationship between obesity and family history and blood uric acid levels in patients aged above 30 years with hyperuricemia at Bangli General Hospital. This study was an analytic study with cross-sectional design. The study samples were 77 people selected by simple random sampling. Secondary data was taken from the patients’ medical records at Bangli General Hospital. Levels of blood uric acid were measured using auto check GCU, obesity was measured based on body mass index using microtoise tools to measure height and weight, while family history was obtained by interviews. The data were analyzed using bivariate analysis with Chi-square test (α=0,05). Results of obesity measurement showed that more of the study subjects had obesity (49 persons or 63,6%) and more (46 persons or 59,7%) had family history. The study results showed that there was no relation between obesity and blood uric acid level (p=0,060) while there was relation between family history and blood uric acid level (p=0,006). Keywords: Keywords: Hyperuricemia, obesity, family history. PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

253	Biology	ABS-976
The Relationship between the Level of Knowledge, Attitudes and Behavior in Patients with Sexually Transmitted Diseases (STDs) at Abang I Health Center, Karangasem Ni Nengah Diah Paramitha Purnama Dewi S1, Sagung Putri Permana Lestari2 Murdhana Putere Universitas Warmadewa Abstract Abstract Sexually-transmitted diseases (STDs) are a group of diseases which are primarily transmitted through sexual contact. STDs become very serious because they may occur with a wide coverage in the world. STDs easily spread from one person to another. The low level of knowledge, attitudes and behavior of the community regarding STDs causes the case to be more of big concern. This study aimed to determine the relationship between knowledge, attitude, and behavior in patient of STDs at Puskesmas Abang I, Karangasem. The study method used was cross-sectional analytic with questionnaires as the instrument. The variables studied were knowledge, attitude, behavior, and occurrence of STDs. The study samples were STDs patients who visited the Puskesmas Abang I, who fulfilled the inclusion and exclusion criteria, numbering 49 people. Sampling was carried out by consecutive sampling method. The collected data were analyzed by using computerized program with Chi-Square test (α = 0,05). The result indicated that there was no relationship between knowledge and attitude (p=1,000), knowledge and behavior (p=0,669) and attitude and behavior (p=0,299). There is a need for counseling to be conducted by Puskesmas Abang I and the need for a program from the Ministry of Health to reduce the occurrence of STDs. Keywords: Keywords: Knowledge, Attitude, Behavior, STDs PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

254	Biology	ABS-721
Teaching Material Development of Alam Sekitar Based on Mobile Learning on Elementary School. Hadi Hardiansyah1, Rusmono2 , Murni Winarsih 3 UNIVERSITAS NEGERI JAKARTA Abstract Education and technology must run together in this millenial century. This study aims to develop teaching materials based on mobile learning for thematic learning in elementary school. This study uses research and development methods by Dick and Carey model. The instruments used in this study were interviews, questionnaires, and tests. The result of this study are teaching materials based on mobile learning. Based on the results of the pre-test and post-test analysis, it showed that there were differences in learning outcomes between the pre-test and post-test stages. The results of the t-test show that t count = 12.10 and t table = 1.99 at the significance level α = 0.05 / 95%. Thus it can be concluded that there is an increase in learning outcomes experienced by students, after participating in thematic learning with teaching materials based on mobile learning. Keywords: teaching materials, mobile learning, alam sekitar. PermaLink | Plain Format | Corresponding Author (Rusmono Rus)

255	Biology	ABS-977
The Level of Knowledge, Attitudes and Behavior of the Head of the Family towards Diarrheal Diseases in Banjar Dauh Uma Bitera Village, Gianyar Regency Pande Ayu Catherina Trieani Subrata, dr. I Wayan Kandera Universitas Warmadewa Abstract Abstract Diarrhea is still a health problem in developing countries including Indonesia according to the health department, the problem is evident from the high morbidity and mortality due to diarrhea. The purpose of this study is to describe the level of knowledge, attitudes, and behaviors from head of household about diarrhea in, Banjar Dauh Uma, Bitera Village Gianyar. This research design use descriptive with cross sectional approach, the number of samples are 70 respondents which is selected by simple random sampling. Data that used in this study is primary data that collected by doing an interview and observation directly to the respondents by using questionnaire and checklist as the instruments. The result of this research suggest that the level of knowledge from respondents is classified good category which is 60 respondents (85,7%). Attitudes from the respondents classified as good category which is 48 respondents (68,6%), and the behavior from the respondents classified sufficient category which is 36 respondents (51,4%). Respondents are less aware about prevention of diarrhea such as having clean water and early handling of diarrhea using oralit. As a conclusions, this research showed that the level of knowledge and attitudes of respondents is classified as good category and the behaviors is classified sufficient category. Therefore expected that paramedics can provide information and health promotion to increase public knowledge about diarrhea. Keywords: Keywords: Knowledge, Attitudes, Behaviors, Diarrhea PermaLink | Plain Format | Corresponding Author (Mirsa Umiyati)

256	Biology	ABS-722
EFFECT OF TEACHING MATERIALS BASED ON MOBILE LEARNING TO LEARNING OUTCOMES OF ALAM SEKITAR Rusmono1, Hadi Hardiansyah2, Murni Winarsih 3 UNIVERSITAS NEGERI JAKARTA Abstract Technological advances have a big influence in the world of education. This study aims to determine the effect of mobile learning-based teaching materials on student learning outcomes in natural material. The experimental research method with a sample of 40 people in second grade elementary school students. The results obtained indicate an increase in student learning outcomes in the surrounding natural material after using teaching materials based on mobile learning. The conclusion of the study, there is a positive influence on the use of mobile learning-based teaching materials on the learning outcomes of the environment. Keywords: teaching materials, mobile learning, alam sekitar. PermaLink | Plain Format | Corresponding Author (Rusmono Rus)

257	Biology	ABS-978
Setting of cash pruning time and giving rabbit urine to growth and results of plant melon (Cucumis melo L.) I Ketut Sunadra (a), Anak Agung Ngurah Mayun Wirajaya (a*), Luh Kartini (a), Made Sri Yuliartini (a) a) Agrotechnology Study Program, Faculty of Agriculture-Warmadewa University * Email: mawir61[at]yahoo.com Abstract The purpose of this study was to obtain the right time for pruning and best urine treatment intervals for the growth and yield of melon plants. This research is a factorial experiment, with the basic design of Randomized Block Design (RBD) with 2 factors. This experiment was carried out in a greenhouse from June to September 2018. The interaction between setting the pruning time (P) and giving rabbit urine (U) to the growth and yield of melon plants on all influential variables was not significant. The highest weight obtained from the weight of fruit per plant because the setting of shoot trimming time is given at P1 of 1,555.21 g or increased by 10.12% compared to the lowest yield at P3 which is 1412.34 g. Fruit weight per plant in U1 urine administration with a given interval of 3 days showed the highest value of 1568.88 g or increased by 14.67% compared to the lowest result at U0 which was 1368.17 g. Keywords: Pruning time, rabbit urine, melon plants PermaLink | Plain Format | Corresponding Author (Anak Agung Ngurah Mayun Wirajaya)

258	Biology	ABS-979
Effect of several types of sludge resulting from activities and concentration on growth and yield of Brassica juncea I Gusti Agus Maha Putra Sanjaya (a), Ketut Irianto (b*) a) Animal Husbandry Department, Agriculture Faculty, Warmadewa University, Bali-Indonesia b) Agrotechnology Department, Agriculture Faculty, Warmadewa University, Bali-Indonesia * Email: iriantoketut[at]yahoo.co.id Abstract The aims of this study were to analyze and test several types of activated sludge to find out the safe and environmentally friendly fertilizer raw material standards, analyze the characteristics and number of activated sludge components that are potentially safe to use and find out fertilizer raw material standards and also test the response of plants witch use the several types of activated sludge in supporting the growth and plant yields that are safe for consumption. The method used is the potensial study using a comparative method. The amount of raw materials and types of fertilizer raw materials using survey methods and mathematical calculations. Result indicated that the process of wastewater treatment technology that uses waste technology is classified as good and were use standards operational with utilization concept. Wastewater in terms of quality and characteristics is considered safe and has a large potential to be used as raw material for fertilizer. Fertilizers sourced from the results of wastewater treatment respond to the fresh weight of leaves of Brassica juncea is higher if compared to Bio Fertilizer and Chemical Fertilizer at the same dosage. Keywords: Active sludge, concentration, plant response PermaLink | Plain Format | Corresponding Author (I Gusti Agus Maha Putra Sanjaya)

259	Biology	ABS-212
Analysis of Sound Frequency Exposure at Growing phase of Chrysanthemum Sp. (Case Study: Exposure by Quran Recitation) Liberty Chaidir, Lia Kamelia, Aditya Rahman Faculty of Science and Technology UIN Sunan Gunung Djati Bandung Abstract Sound wave technology or Sonic Bloom technology has long been applied to plants. Sound waves affected the plants at different frequencies, sound pressure levels, presentation periods, and distances from sound sources. The aim of this research is to determine the effect of sound technology exposure on certain frequencies on the beginning of shoot growth, plantlet height, leaf number, and stomata opening width . The experiment was conducted by comparing chrysanthemum plants exposed to quran recitation (Surah Al-Fatihah) at an average frequency of 1237.8 Hz for 2 hours for 8 Weeks After Culture (WAC). The results showed that murattal and media interactions occurred in planlet height after 4 WAC and leaf number after 8 WAC. The exposure of murattal had affected on plantlet height after 2 WAC. The treatment influenced the number of leaves after 4 WAC, and the number of roots after 6 WAC. In testing the opening of the stomata, the leaves that given murattal al-Fatihah treatment, having a stomata opening wider than the leaves that were not treated (control). This study is the beginning of research to find the right frequency to stimulate growth in plants. Keywords: Chrysanthemum Sp.;Frequency;Growth;Quran recitation; PermaLink | Plain Format | Corresponding Author (Lia Kamelia)

260	Biology	ABS-724
The Effect of Vermicompost Application on The Growth of Lettuce Plant (Lactuca sativa L.) Budy Frasetya (a*), Kundang Harisman (a), Soheh Maulid (a), Salamet Ginandjar (a) a) Agrotechnology Department, UIN Sunan Gunung Djati Bandung Jalan A. H. Nasution No. 105 Bandung 40614, West Java, Indonesia *budyftq1682[at]gmail.com Abstract The application of salt fertilizer to increase the productivity of lettuce in long term application will decline soil fertility. Implementation of totally organic farming in some region cannot increase productivity rapidly. Combine application of vermicompost with salt fertilizer is a solution to answer decline lettuce production on the first time implementation of totally organic farming. This research aims to study the dose application of vermicompost on the growth of lettuce plant. The research conducted at Cisurupan-Garut West Java Province with altitude 1183 m above sea level from January to March 2018. The method used in this research is randomize block design with seven treatments (A= without vermicompost, B= 5, C= 10, D= 15, E= 20, F=25, G= 30 t ha-1), and four replication. The growth parameter observes in this research is plant height, number of leaves, fresh plant weight, plant dry weight, and shoot-root ratio. The data were collected from the observed parameter than analyzed with variance analyze at five percent signification level. The result of this research shows that the application of 5 t ha-1 vermicompost improves plant growth significantly. Keywords: Vermicompost, totally organic farming, soil fertility PermaLink | Plain Format | Corresponding Author (Budy Frasetya)

261	Biology	ABS-980
Pramenstrual syndrome profile in high school students at Blahbatuh Gianyar Ida Ayu Diah Wedawati, I Gusti Rai Tirta Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Pramenstrual syndrome (PMS) is a collection of physical, psychological and emotional symptoms associated with a womans menstrual cycle; symptoms usually occur 6-10 days before menstruation and disappear when menstruation begins. Generally the impact of the STDs is the disruption of daily activities. Especially for young women who attend school, STDs can interfere with the quality of health, concentration, achievement and active learning activities in schools. This research was conducted in October 2017 until December 2017 at SMAN 1 Blahbatuh. This research is descriptive research using cross sectional approach. The sample in this study amounted to 105 students of class X which was obtained by simple random sampling method. Based on sampling result, got sample 105 people, with sampling method using lottery. The result of the research shows that female students experienced PMS 71,8%. The severity of PMS symptoms in moderate to severe levels is 69.5%. Most girls experienced their first menstruation at the age of 12-13 years as much as 65.7%. Based on the results of this study, the researchers suggested to the school to be able to provide health promotion either directly through counseling, or indirectly through health media associated with premenstrual syndrome. Keywords: Premenstrual syndrome, student, high school PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

262	Biology	ABS-981
Risk Factors of Low Birth Weight (LBW) in Kecamatan Tegallalang on 2015-2017 I Gusti Lanang Rama Dwi Suputra, I Gusti Rai tirta Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Low birth weight babies (LBW) are babies born with weight less than 2500 grams regardless of gestational age. LBW is a health problem in Indonesia. Infant mortality rate (IMR) in Indonesia is still high and the main cause is LBW. Kabupaten Gianyar had the highest IMR in Bali that is 12,3 per 1000 live birth. The highest prevalence of LBW in Kabupaten Gianyar on 2016 occurred in Kecamatan Tegallalang. LBW is influenced by several risk factors such as anemia, age, parity, cigarette exposure, hypertension, inadequate antenatal care, poor mothers nutritional status and poor health service quality. The purpose of this study was to determine the risk factor of LBW in Kecamatan Tegallalang on 2015-2017. This study was an analytic study using case control design with univariate, bivariate and multivariate analysis. The independent variables are maternal age, parity, anemia, and hypertension. The research instrument is form checklist. The source of data was secondary data from medical record and kohort book at Puskesmas Tegallalang I and II. The result of bivariate analysis showed that there was a significant correlation between maternal age (p = 0,004; OR = 4,385), parity (p = 0,005; OR = 4,000) and hypertension (p = 0,030; OR = 9,514) with the incidence of LBW. While anemia has no significant correlation with LBW (p = 0,616). In multivariate analysis, the most significant risk factor was hypertension. It is expected that this report can be learned for pregnant women and health provider. Keywords: LBW, antenatal care, case control PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

263	Biology	ABS-982
Knowledge of HIV / AIDS and Attitudes towards People with HIV from Community Leaders in South Denpasar Gusti Ayu Made Sardina Adelia Purwanti, Made Bagus Toya Ariawan, Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Acquired Immune Deficiency Syndrome (AIDS) is a collection of symptoms caused by Human Immunodeficiency Virus (HIV). Bali is the 4th region with the largest number of HIV cases in Indonesia. Data from the Denpasar Health Office stated that the highest number of HIV / AIDS cases was in South Denpasar District with 558 cases. Community leaders as a sample in this study considered to have a great influence on the community in the environment. The purpose of this research is to identify knowledge about HIV / AIDS and attitude of public figure to PLHIV patient especially in South Denpasar. This research is descriptive with cross sectional approach to 96 public figures in South Denpasar selected by using multi-stage random sampling technique. Data collected through questionnaires that have been tested for validity and reliability. The result of community leaders who have good knowledge as many as 39 respondents (40.6%), quite as much as 57 respondents (59.4%) but there are no respondents with less knowledge category. In the category of attitude there are 50 respondents (52.1%) with good category, 39 respondents (40.6%) with enough category and 7 respondents (7.3%) with less category. This study is expected to be useful as a basis for further education to reduce transmission and increase HIV / AIDS prevention efforts and reduce the negative stigma and rejection of PLWHA and theoretically can be a reference in subsequent research. Keywords: Knowledge, Attitude, PLWHA, HIV / AIDS PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

264	Biology	ABS-727
Identification of Cardiovascular Risk Factors among Hajj Pilgrim from Bali NW Widhidewi, S Masyeni, A Eka Pratiwi Faculty of Medicine and Health Sciences, Universitas Warmadewa, Denpasar, Bali Abstract Almost 2 million Muslims from more than 183 countries conduct Hajj to Mecca, Saudi Arabia every year. This event carries significant public health challenges not only infectious disease transmission but also exacerbation of non-communicable diseases. Although the prevalence of cardiovascular disease (CVD) was not high, it confers high mortality rate. The aim of this study is to identify major risk factors of cardiovascular event among Hajj pilgrims from Bali in 2018. A cross sectional study was conducted in Western Denpasar Community Health Center, where the Hajj preparations take place. Demographical data, history of preexisting medical illness, physical examination, laboratory, electrocardiogram (ECG), thorax x-ray results were collected. CVD risk defined as the finding of at least one of the parameters (age ≥ 55 years old, hypertension, diabetes mellitus, dyslipidemia, hyperuricemia, ECG or x-ray abnormalities). A total of 99 participants were enrolled in the study. Among them, 97 (98%) participants had at least one CVD risk factor. Significant findings of Hajj Pilgrimage with CVD risk implicate that the CVD risk factors identification are profoundly recommended to avoid mortality and morbidity related to cardiovascular event during Hajj-period. Keywords: Hajj pilgrim; risk; cardiovascular disease PermaLink | Plain Format | Corresponding Author (Ni Wayan Widhidewi)

265	Biology	ABS-983
Effect of Spirit of Universal Life (SOUL) Prevention and Healing Meditation on Blood Glucose Level in Patients with Type 2 Diabetes Mellitus Anak Agung Diyananda Paramita(a), I Gusti Ayu Diah Febriyani Sidhiartha(b), Made Sinta Sri Mahadewi(c), Kartika Sari(d) a,b,c,d) Faculty of Medicine and Health Sciences, Warmadewa University Abstract Type 2 diabetes mellitus (T2DM) is a common chronic disease and also a major worldwide public health problem. A wide variety of lifestyle factors play an important role in the development of T2DM, such as sedentary lifestyle, smoking, alcohol consumption, and stress. Psychological stress is being explored as a risk factor for chronic diseases, such as cardiovascular disease, arthritis and diabetes. Pathophysiological mechanisms that link stress with diabetes involve direct neuroendocrine effects, in which stress-associated hormones such as cortisol and adrenaline have regulatory effect on insulin release. Cortisol can cause glucose formation in peripheral tissues as well as in the liver. This condition causes increased blood glucose level in T2DM. The objective of this study was to assess the effectiveness of stress management by SOUL Prevention and Healing meditation to normalize blood glucose level in DMT2 patients. This study used a quasi experimental design; the study sample comprised 16 volunteers who made all the inclusion criteria. This study was conducted in Bali Royal Hospital (BROS) for 2 months. Fasting blood samples were collected three times (pre-treatment, 1 months post treatment, 2 months post treatment). Brain waves of each volunteer were recorded by using EEG before and after treatment. The study results showed that the average reduction of blood sugar level was 48.62 mg/dl (p value, p <0.05 (p = 0.001)). The EEG brain waves recording showed that all volunteers had achieved delta frequency. It is concluded that the brain wave delta frequency and normalized blood glucose level indicate a significant result of SOUL Prevention and Healing meditation. Keywords: Meditation, T2DM, Blood glucose level PermaLink | Plain Format | Corresponding Author (Ni Luh Putu Eka Kartika Sari)

266	Biology	ABS-984
Level of Knowledge, Attitude and Behavior of Housewives about Mosquito Nest Eradication in Gianyar Regency in 2017 Gede Ricky Ananta Herryadi, Made Bagus Toya Ariawan Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Dengue Hemorrhagic Fever (DHF) is still a health problem in the world including Indonesia. Province in Indonesia with the highest DHF rate in 2015 is Bali. The number of dengue fever cases continues to increase in Gianyar Regency. This is not separated from the role of housewives in taking care of the house. The purpose of this research is to know the level of knowledge, attitude and behavior of housewife about mosquito nest eradication in Gianyar Regency in 2017. This research is a descriptive research conducted by using cross sectional method. The sample of this study is 106 housewives selected by multistage random sampling method. The results of this research indicates that the knowledge rates of housewives about mosquito nest eradication is enough 50.9%, less 29.2%, and good 19.8%. The results of housewife attitudes shows enough attitudes at 75.5%, good 20.8% and less 3.8%. For the results of housewives behavior reveals enough behavior 58.5%, less 34.9%, and good 6.6%. The results showed that the knowledge levels, attitudes, and behaviors of housewives in the Batubulan Village, Sukawati District, Gianyar Regency about mosquito nest eradication reveals enough. Therefore, counseling about mosquito nest eradication activities should be done to improve knowledge, attitude and behavior of housewives. In addition it is suggested all parties take an active role in conducting mosquito nest eradication activities in order to improve the degree of health. Keywords: DHF, Knowledge, Attitude, Behavior, Mosquito Nest Eradication PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

267	Biology	ABS-729
the effect of energy and protein balances on the growth female garut lamb Pri Riznaya, Ana Rochana, Diding Latipudin Universitas Padjadjaran Abstract The purpose of this research was to determine the effect of energy and protein balances on the growth female Garut lamb. The variables are daily consumption, daily weight gain, and conversion ratio value. The treatments given included R1 (12% protein and 60% TDN), R2 (12% protein and 65% TDN), R3 (14% protein and 60% TDN), R4 (14% protein and 65% TDN), R5 (16% protein and 60% TDN), and R6 (16% protein and 65% TDN). The feed are arrange using field grass, fine bran, onggok, soy sauce pulp, coconut cake, and premix. The design of the room uses a Completely Randomized Design (CRD), with 4 (four) replications. Data analysis used variance analysis and Duncans multiple analysis. The results showed that various protein and energy ratio had significant effect on daily consumption (whereas R1, R3, and R5 gave better results), but did not affect the daily weight gain and feed conversion ratio value. Keywords: rut female lamb, protein and energy balances, growth PermaLink | Plain Format | Corresponding Author (Pri Riznaya)

268	Biology	ABS-985
The Effects of Boat Engine Noise on The Hearing of Fishermen in Kedonganan Village, Bali Nyoman Sitra Tresna Pagerartha(a), I Gusti Ayu Diah Febriyani Sidiartha(b), Anak Agung Ayu Sri Vemi Hendrayani(c), Agus Santosa(d), Katika Sari(e) a,b,c,d,e) Faculty of Medicine and Health Sciences, Warmadewa University Abstract Noise-induced hearing loss (NIHL) is the common occupational disease especially for fishermen who using boat for fishing. NIHL is a permanent hearing abnormality caused by prolonged exposure to loud noise and the first symptom is normally the inability to hear high-pitched sounds. The Regulation of Indonesian Ministry of Health 2016, mentioned the safe range of noise maximum is 88 dB with the maximum time of exposure is 4 hours. Meanwhile, the noise caused by boat engine is 77,64-97,8 dB. The objective of this study was to assess the risk factor of boat engine noise towards NIHL in fishermen. Audiometric examination was done to evaluate the samples with normal baseline, 25 dB. The study sample comprised 70 volunteers which devided within 2 groups (fisherman and 35 local resident-non fisherman). This study was conducted in Kedonganan Village, Bali. The study results showed that 77% (27 of 35) of fisherman is diagnosed as NIHL with working hour average ±4,86 hours and ±16 years period of exposure. Group with non fishermen showed 2 NIHL condition eithout any exposure from boat-engine (p<0.05). It is concluded that the the noise from boat engine can cause hearing loss. The fisherman have to wear earplug or earmuff or headset to reduce the risk of noise induced hearing loss. Keywords: NIHL, noise, fishermen PermaLink | Plain Format | Corresponding Author (Ni Luh Putu Eka Kartika Sari)

269	Biology	ABS-730
BAGGASSE CHARCOAL OPTIMATION BASED ON DIFFERENT CONCENTRATION AND IMMERSING TIME : IT USE IN STABILIZING COOKING OIL QUALITY Sri Rahayu, Farid Pujiono, Maharani Dewi, Arief Prasetiyo Department of Biology, Faculty of Mathematics and Natural Sciences Universitas Negeri Jakarta, Indonesia Coresponding author: srirahayu[at]unj.ac.id Abstract Sugar cane is a raw material for making sugar which can only be grown in tropical regions. Sugarcane bagasse generally consist of cellulose sugar groups such as hemicellulose, lignocellulose, and lignin. other than that it contains hydroxyl groups to absorb colors and free fatty acids. The purpose of this study was to measure baggase charcoal optimation based on different concentration and immersing time to stabilize cooking oil quality. CRD (Complete Randomized Design) was applied with 3x3 factors. Baggase charcoal was activated using KMnO4 1N for 2 days. Variation of baggase charcoal concentration are (5%, 10%, 15%) and variation time to optimation for 4, 5, and 6 days. The result showed that baggase charcoal affected to decrease FFA (Free Fatty Acid). Furthermore it also affected with the turbidity of used cooking oil. Keywords: sugar cane, cellulose, hydroxyl, free fatty acid PermaLink | Plain Format | Corresponding Author (Farid Pujiono)

270	Biology	ABS-986
Factors Affecting Sunscreen Usage In 5th Semester Students at Ganesha University of Education Singaraja Year 2017 Arya Putra Kusuma Wardhana, Agus Santosa Faculty of Medicine and Health Sciences, University of Warmadewa Abstract Ultraviolet rays (UV) have various radiation waves, namely: UVA, UVB, UVC, infrared, and visible light with their respective effects on the skin. UVA radiation can penetrate the window glass and can react to chemicals of topical drugs and oral drugs which can cause a reaction called photoallergy and phototoxic reactions. Continuous radiation from UVA and UVB can cause degeneration of connective tissues in the skin such as sun-aging (photoaging), skin cancer (photocarcinogenesis), and immunosuppression on transplantation. Based on data from the Meteorology, Climatology and Geophysics Center, Buleleng Regency is one of the districts in Bali which has a high level of sun exposure intensity as compared with other districts. The purpose of this study were to determine the factors that influence the use of sunscreen by students of Ganesha University of Education. This study uses analytical observational method with cross sectional design, using interview based on prepared and standardized questionnaire. The technique used for sample selection was systematic random sampling in 5th semester students from 7 faculties with a total of 96 samples. The data were analyzed using chi square test including to assess the p value. The results showed a significant association between knowledge level and sunscreen usage (p = 0,003), and lifestyle (p = 0,033). It is expected that people would be able to increase their usage of sunscreen in their own arUltraviolet rays (UV) have various radiation waves, namely: UVA, UVB, UVC, infrared, and visible light with their respective effects on the skin. UVA radiation can penetrate the window glass and can react to chemicals of topical drugs and oral drugs which can cause a reaction called photoallergy and phototoxic reactions. Continuous radiation from UVA and UVB can cause degeneration of connective tissues in the skin such as sun-aging (photoaging), skin cancer (photocarcinogenesis), and immunosuppression on transplantation. Based on data from the Meteorology, Climatology and Geophysics Center, Buleleng Regency is one of the districts in Bali which has a high level of sun exposure intensity as compared with other districts. The purpose of this study were to determine the factors that influence the use of sunscreen by students of Ganesha University of Education. This study uses analytical observational method with cross sectional design, using interview based on prepared and standardized questionnaire. The technique used for sample selection was systematic random sampling in 5th semester students from 7 faculties with a total of 96 samples. The data were analyzed using chi square test including to assess the p value. The results showed a significant association between knowledge level and sunscreen usage (p = 0,003), and lifestyle (p = 0,033). It is expected that people would be able to increase their usage of sunscreen in their own areas through education regarding the function and danger of sunlight as well as maintaining a healthy lifestyle. Keywords: sunscreen, skin cancer, premature aging, ultraviolet rays PermaLink | Plain Format | Corresponding Author (Dewa Ayu Putri Sri Masyeni)

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